Cotton leaf curl Kokhran virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3Y1N4|D3Y1N4_9GEMI Replication enhancer protein OS=Cotton leaf curl Kokhran virus OX=222464 GN=AC2 PE=3 SV=1
MM1 pKa = 8.25DD2 pKa = 3.98SRR4 pKa = 11.84TRR6 pKa = 11.84EE7 pKa = 4.33PITLQPCRR15 pKa = 11.84MSHH18 pKa = 5.35NLEE21 pKa = 3.84VPIPLFQNHH30 pKa = 5.62QPRR33 pKa = 11.84QPSIHH38 pKa = 7.24DD39 pKa = 3.67EE40 pKa = 3.82YY41 pKa = 11.2GYY43 pKa = 8.04THH45 pKa = 7.19DD46 pKa = 4.15QDD48 pKa = 4.47PVQSQPEE55 pKa = 4.17EE56 pKa = 4.43SVGDD60 pKa = 3.51TQMFHH65 pKa = 7.1SFPNLDD71 pKa = 4.87DD72 pKa = 3.97LTASDD77 pKa = 3.38WSFLKK82 pKa = 10.82GLYY85 pKa = 10.01DD86 pKa = 3.72SSASIFNLLRR96 pKa = 11.84NCQYY100 pKa = 11.44YY101 pKa = 10.23IMCLSSWSCIMEE113 pKa = 5.02CITEE117 pKa = 4.09HH118 pKa = 5.74VVYY121 pKa = 10.52VDD123 pKa = 3.35QSSSIKK129 pKa = 10.4FNIYY133 pKa = 10.21

Molecular weight: 15.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3Y1N5|D3Y1N5_9GEMI Rep protein OS=Cotton leaf curl Kokhran virus OX=222464 GN=AC1 PE=3 SV=1
MM1 pKa = 7.79RR2 pKa = 11.84SSSLSKK8 pKa = 10.39DD9 pKa = 2.76HH10 pKa = 6.52CTQLSIKK17 pKa = 8.75VQHH20 pKa = 6.48RR21 pKa = 11.84EE22 pKa = 3.8ARR24 pKa = 11.84DD25 pKa = 3.46ATGEE29 pKa = 4.24RR30 pKa = 11.84IRR32 pKa = 11.84SCSPCYY38 pKa = 9.88SSSVITATPWIHH50 pKa = 6.43AQGNPSLCSLAGCRR64 pKa = 11.84IIWRR68 pKa = 11.84FPSLYY73 pKa = 10.06FRR75 pKa = 11.84IISHH79 pKa = 5.6VNRR82 pKa = 11.84PFTTNMDD89 pKa = 3.21ILTIRR94 pKa = 11.84IQFNHH99 pKa = 6.33NLRR102 pKa = 11.84KK103 pKa = 9.8ALGIHH108 pKa = 6.03KK109 pKa = 9.67CFIAFRR115 pKa = 11.84IWMTSQPPTGRR126 pKa = 11.84FLRR129 pKa = 11.84VFTTQVLQYY138 pKa = 10.18LIYY141 pKa = 10.43LGIVSIII148 pKa = 3.43

Molecular weight: 17.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1125

112

358

187.5

21.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.533 ± 0.691

3.2 ± 0.505

4.356 ± 0.557

3.556 ± 0.53

4.8 ± 0.599

4.444 ± 0.575

3.733 ± 0.349

5.689 ± 0.977

5.244 ± 1.132

6.578 ± 0.844

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.4 ± 0.494

4.533 ± 0.475

5.156 ± 0.423

5.333 ± 0.84

8.622 ± 0.86

11.378 ± 1.31

5.778 ± 0.571

5.244 ± 1.396

1.422 ± 0.116

4.0 ± 0.546
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski