Rice stripe necrosis virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Hepelivirales; Benyviridae; Benyvirus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|B5STA0|B5STA0_9VIRU 12K protein OS=Rice stripe necrosis virus OX=373373 GN=TGBp2 PE=4 SV=1
MM1 pKa = 6.36TSEE4 pKa = 4.24WARR7 pKa = 11.84EE8 pKa = 3.87HH9 pKa = 6.1PTDD12 pKa = 3.23VFAIFEE18 pKa = 4.32TCARR22 pKa = 11.84EE23 pKa = 5.74AGFTWNDD30 pKa = 3.35VPPHH34 pKa = 5.41VVNFDD39 pKa = 3.57SLEE42 pKa = 3.92KK43 pKa = 10.78SKK45 pKa = 11.05AISNLTDD52 pKa = 3.66LLEE55 pKa = 4.68NEE57 pKa = 4.36VAKK60 pKa = 10.78GCKK63 pKa = 9.46EE64 pKa = 4.09KK65 pKa = 11.28GDD67 pKa = 3.51VAAIKK72 pKa = 10.55LEE74 pKa = 4.1NVNGMTKK81 pKa = 10.07EE82 pKa = 3.69YY83 pKa = 9.19NARR86 pKa = 11.84VGVCVGAPGAGKK98 pKa = 6.85TTLIKK103 pKa = 10.74AVMSKK108 pKa = 10.73ASRR111 pKa = 11.84VVIAVPNSTLLKK123 pKa = 10.21NVYY126 pKa = 9.84SGNPNAFLIDD136 pKa = 4.0DD137 pKa = 4.58LFSRR141 pKa = 11.84PVEE144 pKa = 3.78FAKK147 pKa = 11.17YY148 pKa = 7.38EE149 pKa = 4.16TILIDD154 pKa = 3.79EE155 pKa = 4.47FTKK158 pKa = 10.54VHH160 pKa = 4.94VCEE163 pKa = 4.07VLMLSALLRR172 pKa = 11.84VKK174 pKa = 10.51NILMFGDD181 pKa = 4.33PQQGMHH187 pKa = 6.0YY188 pKa = 9.81RR189 pKa = 11.84PGSFVYY195 pKa = 10.97YY196 pKa = 9.8NFPVLAEE203 pKa = 4.01SHH205 pKa = 6.52ASHH208 pKa = 7.1RR209 pKa = 11.84MPQAIGEE216 pKa = 4.36AYY218 pKa = 9.83NNAMGTKK225 pKa = 9.21IEE227 pKa = 4.48PKK229 pKa = 9.86SSQSGEE235 pKa = 4.1FEE237 pKa = 4.15IKK239 pKa = 10.47DD240 pKa = 3.97LLGMIRR246 pKa = 11.84DD247 pKa = 3.83KK248 pKa = 11.64SKK250 pKa = 10.92VLCLSEE256 pKa = 4.19KK257 pKa = 9.2TQSNLDD263 pKa = 3.5DD264 pKa = 4.77CGISAEE270 pKa = 4.54LVSKK274 pKa = 10.66VQGCEE279 pKa = 3.67FAAVTLILEE288 pKa = 4.65EE289 pKa = 4.48PQDD292 pKa = 3.63IAPFCNKK299 pKa = 10.25SIRR302 pKa = 11.84CVALSRR308 pKa = 11.84AKK310 pKa = 10.18EE311 pKa = 3.9VLIIQATPYY320 pKa = 9.35FKK322 pKa = 11.33SMLCNAEE329 pKa = 4.13FVDD332 pKa = 4.74PYY334 pKa = 10.87EE335 pKa = 4.4VDD337 pKa = 3.74SSCSGQTLCNSRR349 pKa = 3.74

Molecular weight:
38.42 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|B5STA1|B5STA1_9VIRU 15K protein OS=Rice stripe necrosis virus OX=373373 GN=TGBp3 PE=4 SV=2
MM1 pKa = 7.35SGQYY5 pKa = 10.44VSARR9 pKa = 11.84PNKK12 pKa = 9.89FIPICICIGVVCVAVCLVLATPRR35 pKa = 11.84HH36 pKa = 5.19KK37 pKa = 9.4THH39 pKa = 6.51SAGDD43 pKa = 3.61YY44 pKa = 9.83GVPTFANGGSYY55 pKa = 11.33ADD57 pKa = 3.45GTRR60 pKa = 11.84RR61 pKa = 11.84AKK63 pKa = 10.46FNCNNDD69 pKa = 3.2RR70 pKa = 11.84AYY72 pKa = 10.64GSSQPQMSSNFVAFIAVILLVAFALRR98 pKa = 11.84SCNSVGNCGEE108 pKa = 4.11RR109 pKa = 11.84CNGGCCKK116 pKa = 10.55NN117 pKa = 3.46

Molecular weight:
12.36 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

4177

117

2100

522.1

58.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.283 ± 0.225

1.819 ± 0.648

5.602 ± 0.276

6.416 ± 0.581

4.166 ± 0.302

6.584 ± 0.538

1.891 ± 0.202

4.956 ± 0.306

4.812 ± 0.534

8.188 ± 0.432

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.657 ± 0.293

4.597 ± 0.282

4.98 ± 0.447

3.663 ± 0.41

6.512 ± 0.743

7.709 ± 0.679

4.74 ± 0.237

7.541 ± 0.831

1.269 ± 0.241

3.543 ± 0.258

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski