Mucilaginibacter sp. 94

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Mucilaginibacter; unclassified Mucilaginibacter

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4311 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A495STW6|A0A495STW6_9SPHI 50S ribosomal protein L13 OS=Mucilaginibacter sp. 94 OX=2183922 GN=rplM PE=3 SV=1
MM1 pKa = 8.31DD2 pKa = 4.15YY3 pKa = 11.08NVYY6 pKa = 10.9LLATDD11 pKa = 4.43PNNPCRR17 pKa = 11.84DD18 pKa = 3.81VIHH21 pKa = 6.95SRR23 pKa = 11.84DD24 pKa = 3.42TGLKK28 pKa = 8.94IRR30 pKa = 11.84VYY32 pKa = 10.33CLSEE36 pKa = 4.6DD37 pKa = 3.56SFTPSEE43 pKa = 4.58NEE45 pKa = 3.51IQLYY49 pKa = 10.63GYY51 pKa = 11.24ANDD54 pKa = 3.81KK55 pKa = 10.82LYY57 pKa = 11.05AFEE60 pKa = 5.34TINIMADD67 pKa = 3.33DD68 pKa = 4.49ALDD71 pKa = 3.62VVGAIQWYY79 pKa = 9.61ADD81 pKa = 3.84YY82 pKa = 11.23IEE84 pKa = 4.89FPDD87 pKa = 4.39MEE89 pKa = 5.2ILPEE93 pKa = 4.11DD94 pKa = 3.89PRR96 pKa = 11.84RR97 pKa = 11.84SSDD100 pKa = 2.62IAMM103 pKa = 4.65

Molecular weight: 11.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A495TVY4|A0A495TVY4_9SPHI DoxX-like protein OS=Mucilaginibacter sp. 94 OX=2183922 GN=DFS16_0603 PE=4 SV=1
MM1 pKa = 7.1KK2 pKa = 9.96TFVYY6 pKa = 10.41LVFTCFISTGAFLGALNSKK25 pKa = 8.74TPLLLYY31 pKa = 10.66AVGFGIWGLFLWGLNRR47 pKa = 11.84RR48 pKa = 11.84MKK50 pKa = 10.51KK51 pKa = 9.55KK52 pKa = 10.62AEE54 pKa = 4.03RR55 pKa = 11.84QDD57 pKa = 3.35MEE59 pKa = 5.66RR60 pKa = 11.84NFQNYY65 pKa = 6.9MRR67 pKa = 11.84YY68 pKa = 7.52QARR71 pKa = 11.84RR72 pKa = 11.84PGRR75 pKa = 3.57

Molecular weight: 8.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4311

0

4311

1404697

29

2392

325.8

36.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.891 ± 0.036

0.775 ± 0.011

5.483 ± 0.026

5.339 ± 0.041

4.822 ± 0.028

6.812 ± 0.034

1.887 ± 0.017

7.348 ± 0.034

7.071 ± 0.036

9.429 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.329 ± 0.02

5.735 ± 0.04

3.855 ± 0.022

3.822 ± 0.027

3.811 ± 0.021

5.947 ± 0.03

5.951 ± 0.033

6.421 ± 0.022

1.121 ± 0.015

4.15 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski