Planctomycetes bacterium Pla133

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; unclassified Planctomycetes

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5200 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A518BGU1|A0A518BGU1_9BACT Uncharacterized protein OS=Planctomycetes bacterium Pla133 OX=2528011 GN=Pla133_12740 PE=4 SV=1
MM1 pKa = 7.31HH2 pKa = 7.26FKK4 pKa = 9.82STVLALSLTATAATAQVTVDD24 pKa = 3.98VGPDD28 pKa = 3.56PAPTGATVYY37 pKa = 11.16ASVTNDD43 pKa = 3.06TEE45 pKa = 5.34LIMSIGGCPWRR56 pKa = 11.84IVDD59 pKa = 3.85GDD61 pKa = 4.01GLVVFDD67 pKa = 5.26ADD69 pKa = 4.01CLITEE74 pKa = 4.57FLIGPIGTIDD84 pKa = 3.76YY85 pKa = 10.59GWDD88 pKa = 3.13QRR90 pKa = 11.84NQAGVQVPAGSYY102 pKa = 9.57HH103 pKa = 5.45FQVMTAVGIIDD114 pKa = 3.64TPFTVGGVDD123 pKa = 3.68ANLFVQGTAALGTDD137 pKa = 3.81MIGFGGRR144 pKa = 11.84EE145 pKa = 3.61IALASPADD153 pKa = 3.76PGAFYY158 pKa = 10.59QVLLAGSLGGGVDD171 pKa = 3.85LCGLTMPLAIDD182 pKa = 3.88GLFTATLGGATIPGAFGQLDD202 pKa = 3.72GTGQSLEE209 pKa = 4.27PKK211 pKa = 10.32LPIPDD216 pKa = 3.73APGLVGIDD224 pKa = 4.67LYY226 pKa = 11.72LSMLVLDD233 pKa = 5.18PLASCPVVRR242 pKa = 11.84SSPAFKK248 pKa = 8.88TTIVPGPQPFGSS260 pKa = 3.57

Molecular weight: 26.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A518BJP8|A0A518BJP8_9BACT Histidine--tRNA ligase OS=Planctomycetes bacterium Pla133 OX=2528011 GN=hisS PE=3 SV=1
MM1 pKa = 7.63KK2 pKa = 10.31IKK4 pKa = 10.02IRR6 pKa = 11.84KK7 pKa = 8.71SRR9 pKa = 11.84AKK11 pKa = 9.7HH12 pKa = 5.21AKK14 pKa = 6.11TTGFLSRR21 pKa = 11.84QRR23 pKa = 11.84TKK25 pKa = 11.0GGRR28 pKa = 11.84KK29 pKa = 6.08TNKK32 pKa = 8.9RR33 pKa = 11.84QRR35 pKa = 11.84ARR37 pKa = 11.84HH38 pKa = 5.0GAFF41 pKa = 3.28

Molecular weight: 4.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5200

0

5200

2059126

29

3669

396.0

42.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.574 ± 0.046

0.862 ± 0.01

6.394 ± 0.031

6.474 ± 0.033

3.344 ± 0.018

9.758 ± 0.036

1.922 ± 0.015

3.476 ± 0.021

1.721 ± 0.023

11.251 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.543 ± 0.013

1.9 ± 0.018

5.815 ± 0.024

2.941 ± 0.017

7.917 ± 0.044

5.801 ± 0.027

5.021 ± 0.028

7.905 ± 0.028

1.574 ± 0.015

1.807 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski