Crocosphaera watsonii WH 8501

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Chroococcales; Aphanothecaceae; Crocosphaera; Crocosphaera watsonii

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5659 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4C6L7|Q4C6L7_CROWT Uncharacterized protein OS=Crocosphaera watsonii WH 8501 OX=165597 GN=CwatDRAFT_5003 PE=4 SV=1
MM1 pKa = 7.8LSTTFSGTTSYY12 pKa = 11.09SSSIAYY18 pKa = 9.6GFEE21 pKa = 3.82VEE23 pKa = 4.47NLINSSSSDD32 pKa = 3.4KK33 pKa = 10.81IYY35 pKa = 10.98LNNAANEE42 pKa = 4.02IMGYY46 pKa = 9.48NPNRR50 pKa = 11.84STGQDD55 pKa = 2.86KK56 pKa = 10.41IYY58 pKa = 10.85YY59 pKa = 7.55GTSQDD64 pKa = 3.96TLILDD69 pKa = 3.7YY70 pKa = 11.36AHH72 pKa = 6.82YY73 pKa = 10.28EE74 pKa = 4.13VTEE77 pKa = 4.24SQSGQNLIINLGGNGSVEE95 pKa = 4.08LVDD98 pKa = 5.8YY99 pKa = 11.12YY100 pKa = 11.63ADD102 pKa = 4.18PNNQIQISYY111 pKa = 9.64AIPPTPRR118 pKa = 11.84PEE120 pKa = 4.1PPEE123 pKa = 4.92GIDD126 pKa = 3.78IVIAEE131 pKa = 4.78IGQISNLDD139 pKa = 3.82HH140 pKa = 6.75NNQTIVLDD148 pKa = 3.74HH149 pKa = 7.21DD150 pKa = 4.09FVNPVILAQPLSFNDD165 pKa = 3.4GGVAIARR172 pKa = 11.84ITDD175 pKa = 3.38IQSDD179 pKa = 3.91RR180 pKa = 11.84FSVEE184 pKa = 3.22IQEE187 pKa = 4.7ASNEE191 pKa = 4.17DD192 pKa = 3.84GIHH195 pKa = 4.74TVEE198 pKa = 4.14TFSYY202 pKa = 10.47LVLEE206 pKa = 4.2EE207 pKa = 5.21GIWEE211 pKa = 4.35LSDD214 pKa = 3.2GTIIEE219 pKa = 4.16VGIINATNSSWSNINFSYY237 pKa = 11.15DD238 pKa = 3.34FVDD241 pKa = 4.5DD242 pKa = 4.4PVVLTQIQTNNDD254 pKa = 3.35SNFVKK259 pKa = 9.91TRR261 pKa = 11.84HH262 pKa = 5.8NNITQDD268 pKa = 3.18GFEE271 pKa = 4.29VLLEE275 pKa = 4.12KK276 pKa = 11.09DD277 pKa = 3.37EE278 pKa = 4.95ANNGTSYY285 pKa = 11.13GIEE288 pKa = 3.91TVAWMAISSGTGSWDD303 pKa = 3.05GNTFMAGNTGDD314 pKa = 3.63QVTHH318 pKa = 7.05DD319 pKa = 3.26WHH321 pKa = 6.81TIDD324 pKa = 5.68FGNAFNNTPKK334 pKa = 10.63FLGNIASYY342 pKa = 10.5YY343 pKa = 10.64GPDD346 pKa = 3.64PSGLRR351 pKa = 11.84YY352 pKa = 9.89QNLNNGNVQIKK363 pKa = 10.22IEE365 pKa = 4.37EE366 pKa = 4.24DD367 pKa = 3.11TSADD371 pKa = 3.56SEE373 pKa = 4.36IGHH376 pKa = 5.37TTEE379 pKa = 5.17DD380 pKa = 2.89VDD382 pKa = 3.95YY383 pKa = 10.93LAIEE387 pKa = 4.2ATGTLIGSTYY397 pKa = 11.16GLIPPTSNDD406 pKa = 3.85PIIAQLGQVTNLDD419 pKa = 4.27HH420 pKa = 6.98NNQTIVLDD428 pKa = 3.79HH429 pKa = 7.27DD430 pKa = 4.29FDD432 pKa = 4.83NPVIFANPLSYY443 pKa = 10.87NGPAPSIARR452 pKa = 11.84ITDD455 pKa = 3.14IQGDD459 pKa = 3.96RR460 pKa = 11.84FSVEE464 pKa = 3.65LQEE467 pKa = 4.91PSNEE471 pKa = 4.4DD472 pKa = 2.99GTHH475 pKa = 6.1AEE477 pKa = 4.13EE478 pKa = 4.43TVSFLALEE486 pKa = 4.06EE487 pKa = 5.19GIWEE491 pKa = 4.45LSDD494 pKa = 3.2GTIIEE499 pKa = 4.44VGTIDD504 pKa = 3.56TTHH507 pKa = 6.78SDD509 pKa = 2.49WHH511 pKa = 6.63NINFSYY517 pKa = 11.07DD518 pKa = 3.45FVDD521 pKa = 4.82GPVVLTQIQTNNDD534 pKa = 2.75SSFVKK539 pKa = 9.58TRR541 pKa = 11.84HH542 pKa = 5.81NNITQDD548 pKa = 3.18GFEE551 pKa = 4.28VLLEE555 pKa = 4.07NDD557 pKa = 3.89EE558 pKa = 5.32ANMNSGHH565 pKa = 6.01GNEE568 pKa = 4.16TVAWMAISSGTGSWDD583 pKa = 3.05GNTFMAGNTGDD594 pKa = 3.63QVTHH598 pKa = 7.05DD599 pKa = 3.26WHH601 pKa = 6.81TIDD604 pKa = 5.68FGNAFNNTPKK614 pKa = 10.63FLGNIASYY622 pKa = 10.5YY623 pKa = 10.64GPDD626 pKa = 3.64PSGLRR631 pKa = 11.84YY632 pKa = 9.89QNLNNGNVEE641 pKa = 4.0IKK643 pKa = 10.13IEE645 pKa = 4.0EE646 pKa = 4.97DD647 pKa = 2.83ISIDD651 pKa = 3.82EE652 pKa = 4.53EE653 pKa = 4.51VTHH656 pKa = 5.95ITEE659 pKa = 4.67DD660 pKa = 3.54VHH662 pKa = 7.86FLAIEE667 pKa = 4.26GTGTLTGSTYY677 pKa = 10.2IDD679 pKa = 4.63PDD681 pKa = 3.63NDD683 pKa = 4.03PDD685 pKa = 4.1PVSTIAQVGQITNLDD700 pKa = 3.98EE701 pKa = 4.48NNQTIVLDD709 pKa = 3.79HH710 pKa = 7.27DD711 pKa = 4.29FDD713 pKa = 4.83NPVIFANPLSYY724 pKa = 10.87NGPAPSIARR733 pKa = 11.84ITDD736 pKa = 3.21IQSDD740 pKa = 3.94RR741 pKa = 11.84FSVEE745 pKa = 3.44LQEE748 pKa = 4.91PSNEE752 pKa = 4.4DD753 pKa = 2.99GTHH756 pKa = 6.02AEE758 pKa = 4.15EE759 pKa = 4.46TFSFLALEE767 pKa = 4.36KK768 pKa = 10.77GVWTLSDD775 pKa = 3.32GTVIEE780 pKa = 4.65VGTIDD785 pKa = 3.37TNAIAGSYY793 pKa = 8.56WEE795 pKa = 5.48NITFDD800 pKa = 3.98YY801 pKa = 11.15DD802 pKa = 3.36FTNAPIVLTQVQTDD816 pKa = 3.25NDD818 pKa = 3.47ASFVKK823 pKa = 9.68TRR825 pKa = 11.84QNNITQDD832 pKa = 3.56GFDD835 pKa = 3.85LALEE839 pKa = 4.11NDD841 pKa = 3.82EE842 pKa = 5.55ANLNSGHH849 pKa = 5.25GTEE852 pKa = 4.35TVARR856 pKa = 11.84VAISSGTGDD865 pKa = 3.09WDD867 pKa = 3.85GNTFMAGEE875 pKa = 4.16TGDD878 pKa = 3.84YY879 pKa = 8.78VTEE882 pKa = 4.19AFYY885 pKa = 10.46TLNFGNAFNKK895 pKa = 10.1APKK898 pKa = 9.98FLGNIASYY906 pKa = 10.61YY907 pKa = 10.66GSDD910 pKa = 3.63PSGLRR915 pKa = 11.84YY916 pKa = 9.88QNLNNGNVEE925 pKa = 4.04IKK927 pKa = 10.23IEE929 pKa = 4.17EE930 pKa = 4.15DD931 pKa = 3.18TSIDD935 pKa = 3.48EE936 pKa = 4.65EE937 pKa = 4.71IIHH940 pKa = 5.73ITEE943 pKa = 4.11NVHH946 pKa = 6.4FLAIEE951 pKa = 4.24GTGTLTGSANTGNNDD966 pKa = 3.95PLTGLATEE974 pKa = 4.33QTATASQDD982 pKa = 2.79IFVVGNAQEE991 pKa = 4.27PLYY994 pKa = 9.95DD995 pKa = 3.3TYY997 pKa = 11.74GKK999 pKa = 9.87HH1000 pKa = 7.32DD1001 pKa = 4.01YY1002 pKa = 11.46LEE1004 pKa = 4.25ILGFDD1009 pKa = 3.28QSEE1012 pKa = 4.56DD1013 pKa = 3.72VIQLNGIADD1022 pKa = 4.11NYY1024 pKa = 11.03SLGASPFDD1032 pKa = 4.96SNDD1035 pKa = 2.84QGIFLKK1041 pKa = 10.67VAGMQDD1047 pKa = 3.35EE1048 pKa = 4.53LVAIVKK1054 pKa = 10.24DD1055 pKa = 4.09NNNLDD1060 pKa = 3.93LNSNQFVFVV1069 pKa = 3.68

Molecular weight: 116.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q4C5Z7|Q4C5Z7_CROWT Uncharacterized protein OS=Crocosphaera watsonii WH 8501 OX=165597 GN=CwatDRAFT_4741 PE=4 SV=1
MM1 pKa = 6.61TQRR4 pKa = 11.84TLGGTNRR11 pKa = 11.84KK12 pKa = 7.61QKK14 pKa = 8.99RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84KK25 pKa = 9.23SNGRR29 pKa = 11.84KK30 pKa = 9.11VIQARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.98KK38 pKa = 9.65GRR40 pKa = 11.84HH41 pKa = 5.0RR42 pKa = 11.84LSVV45 pKa = 3.12

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5659

0

5659

1581675

29

2652

279.5

31.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.094 ± 0.037

1.141 ± 0.012

5.045 ± 0.032

6.95 ± 0.033

4.148 ± 0.025

6.178 ± 0.038

1.84 ± 0.015

7.471 ± 0.033

6.791 ± 0.05

10.725 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.848 ± 0.016

4.926 ± 0.03

4.403 ± 0.026

4.879 ± 0.025

4.916 ± 0.029

6.483 ± 0.024

5.421 ± 0.027

5.947 ± 0.027

1.429 ± 0.015

3.363 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski