Holospora undulata HU1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales; Holosporaceae; Holospora; Holospora undulata

Average proteome isoelectric point is 7.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1184 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A061JGD9|A0A061JGD9_9PROT Uncharacterized protein OS=Holospora undulata HU1 OX=1321371 GN=K737_300550 PE=4 SV=1
MM1 pKa = 7.51LIDD4 pKa = 4.19WFQEE8 pKa = 3.97LSDD11 pKa = 4.29CSPLGNHH18 pKa = 6.57QIFLSNFISSNEE30 pKa = 3.69MVRR33 pKa = 11.84FKK35 pKa = 11.1EE36 pKa = 3.98LLKK39 pKa = 10.81SKK41 pKa = 10.36YY42 pKa = 10.41DD43 pKa = 3.3RR44 pKa = 11.84FFSYY48 pKa = 10.64NRR50 pKa = 11.84YY51 pKa = 9.88NSLNQHH57 pKa = 6.02SYY59 pKa = 11.48DD60 pKa = 4.56LIMNNAIFLQTIGDD74 pKa = 3.78NAFVTGIYY82 pKa = 9.87QKK84 pKa = 10.8YY85 pKa = 9.88NEE87 pKa = 4.21WLRR90 pKa = 11.84NSTAPDD96 pKa = 4.04DD97 pKa = 4.94DD98 pKa = 4.91SEE100 pKa = 4.66TVLDD104 pKa = 4.57GNSDD108 pKa = 3.59NYY110 pKa = 11.62DD111 pKa = 3.76DD112 pKa = 5.9FDD114 pKa = 5.82DD115 pKa = 5.87SEE117 pKa = 4.87TEE119 pKa = 4.07MYY121 pKa = 10.86

Molecular weight: 14.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A061JGA4|A0A061JGA4_9PROT Putative HTH-type transcriptional regulator YdcH OS=Holospora undulata HU1 OX=1321371 GN=K737_300635 PE=4 SV=1
MM1 pKa = 7.38ARR3 pKa = 11.84ISGVNIPEE11 pKa = 4.29KK12 pKa = 10.85KK13 pKa = 10.17NLLSGLICIHH23 pKa = 6.74GIGRR27 pKa = 11.84FKK29 pKa = 10.94ALEE32 pKa = 4.04VCRR35 pKa = 11.84SLGLDD40 pKa = 2.74YY41 pKa = 10.59HH42 pKa = 7.22ARR44 pKa = 11.84VSEE47 pKa = 4.08LSEE50 pKa = 3.98KK51 pKa = 10.77DD52 pKa = 2.81IVRR55 pKa = 11.84IRR57 pKa = 11.84EE58 pKa = 4.28MIDD61 pKa = 2.77KK62 pKa = 9.5EE63 pKa = 4.43YY64 pKa = 10.99VVEE67 pKa = 5.17GEE69 pKa = 4.15LRR71 pKa = 11.84NNNLMSKK78 pKa = 9.82KK79 pKa = 10.18RR80 pKa = 11.84LMDD83 pKa = 4.16LGCYY87 pKa = 9.58RR88 pKa = 11.84GLRR91 pKa = 11.84HH92 pKa = 6.56RR93 pKa = 11.84KK94 pKa = 8.43NLPARR99 pKa = 11.84GQRR102 pKa = 11.84THH104 pKa = 5.64TNARR108 pKa = 11.84TRR110 pKa = 11.84RR111 pKa = 11.84GRR113 pKa = 11.84AVAIAGKK120 pKa = 10.21KK121 pKa = 8.86KK122 pKa = 10.57

Molecular weight: 13.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1184

0

1184

309843

29

2435

261.7

29.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.05 ± 0.071

1.685 ± 0.036

4.51 ± 0.066

6.508 ± 0.093

5.559 ± 0.089

6.031 ± 0.089

2.325 ± 0.044

6.908 ± 0.058

7.676 ± 0.107

10.634 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.27 ± 0.032

4.304 ± 0.088

3.827 ± 0.05

4.224 ± 0.059

4.576 ± 0.053

7.674 ± 0.061

4.441 ± 0.054

6.191 ± 0.08

1.393 ± 0.04

3.215 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski