Amino acid dipepetide frequency for Ramazzottius varieornatus (Water bear) (Tardigrade)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.843AlaAla: 6.843 ± 0.043	1.209AlaCys: 1.209 ± 0.014	3.943AlaAsp: 3.943 ± 0.026	4.793AlaGlu: 4.793 ± 0.04	2.995AlaPhe: 2.995 ± 0.021	4.51AlaGly: 4.51 ± 0.033	1.541AlaHis: 1.541 ± 0.014	3.404AlaIle: 3.404 ± 0.027	4.039AlaLys: 4.039 ± 0.028	6.5AlaLeu: 6.5 ± 0.041	1.846AlaMet: 1.846 ± 0.018	2.729AlaAsn: 2.729 ± 0.018	3.772AlaPro: 3.772 ± 0.041	2.932AlaGln: 2.932 ± 0.023	3.912AlaArg: 3.912 ± 0.025	6.25AlaSer: 6.25 ± 0.034	4.476AlaThr: 4.476 ± 0.029	5.565AlaVal: 5.565 ± 0.029	0.764AlaTrp: 0.764 ± 0.011	1.849AlaTyr: 1.849 ± 0.018	0.0AlaXaa: 0.0 ± 0.0
Cys	1.207CysAla: 1.207 ± 0.015	0.509CysCys: 0.509 ± 0.011	0.973CysAsp: 0.973 ± 0.017	0.851CysGlu: 0.851 ± 0.014	0.837CysPhe: 0.837 ± 0.012	1.314CysGly: 1.314 ± 0.018	0.477CysHis: 0.477 ± 0.008	0.872CysIle: 0.872 ± 0.014	0.829CysLys: 0.829 ± 0.012	1.811CysLeu: 1.811 ± 0.019	0.398CysMet: 0.398 ± 0.008	0.703CysAsn: 0.703 ± 0.013	1.031CysPro: 1.031 ± 0.019	0.782CysGln: 0.782 ± 0.014	1.204CysArg: 1.204 ± 0.014	1.539CysSer: 1.539 ± 0.02	0.991CysThr: 0.991 ± 0.012	1.173CysVal: 1.173 ± 0.015	0.276CysTrp: 0.276 ± 0.007	0.55CysTyr: 0.55 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	3.71AspAla: 3.71 ± 0.023	0.971AspCys: 0.971 ± 0.015	3.533AspAsp: 3.533 ± 0.034	3.985AspGlu: 3.985 ± 0.03	2.459AspPhe: 2.459 ± 0.02	3.637AspGly: 3.637 ± 0.03	1.257AspHis: 1.257 ± 0.015	2.818AspIle: 2.818 ± 0.017	2.736AspLys: 2.736 ± 0.022	4.84AspLeu: 4.84 ± 0.028	1.314AspMet: 1.314 ± 0.014	2.059AspAsn: 2.059 ± 0.02	2.589AspPro: 2.589 ± 0.023	2.072AspGln: 2.072 ± 0.016	3.092AspArg: 3.092 ± 0.021	3.923AspSer: 3.923 ± 0.027	2.678AspThr: 2.678 ± 0.018	3.841AspVal: 3.841 ± 0.023	0.683AspTrp: 0.683 ± 0.011	1.692AspTyr: 1.692 ± 0.018	0.0AspXaa: 0.0 ± 0.0
Glu	4.72GluAla: 4.72 ± 0.036	0.894GluCys: 0.894 ± 0.015	3.82GluAsp: 3.82 ± 0.03	5.556GluGlu: 5.556 ± 0.062	1.989GluPhe: 1.989 ± 0.019	3.134GluGly: 3.134 ± 0.025	1.378GluHis: 1.378 ± 0.013	2.916GluIle: 2.916 ± 0.021	4.206GluLys: 4.206 ± 0.038	5.146GluLeu: 5.146 ± 0.037	1.64GluMet: 1.64 ± 0.016	2.686GluAsn: 2.686 ± 0.022	2.574GluPro: 2.574 ± 0.023	2.485GluGln: 2.485 ± 0.028	3.781GluArg: 3.781 ± 0.033	3.943GluSer: 3.943 ± 0.024	3.443GluThr: 3.443 ± 0.047	4.051GluVal: 4.051 ± 0.039	0.751GluTrp: 0.751 ± 0.01	1.715GluTyr: 1.715 ± 0.015	0.0GluXaa: 0.0 ± 0.0
Phe	2.853PheAla: 2.853 ± 0.023	0.944PheCys: 0.944 ± 0.011	2.357PheAsp: 2.357 ± 0.018	2.085PheGlu: 2.085 ± 0.018	1.928PhePhe: 1.928 ± 0.019	2.859PheGly: 2.859 ± 0.024	1.12PheHis: 1.12 ± 0.012	1.858PheIle: 1.858 ± 0.018	1.701PheLys: 1.701 ± 0.016	4.14PheLeu: 4.14 ± 0.029	0.903PheMet: 0.903 ± 0.012	1.546PheAsn: 1.546 ± 0.016	2.206PhePro: 2.206 ± 0.018	1.618PheGln: 1.618 ± 0.013	2.531PheArg: 2.531 ± 0.02	3.369PheSer: 3.369 ± 0.025	2.215PheThr: 2.215 ± 0.019	2.925PheVal: 2.925 ± 0.022	0.547PheTrp: 0.547 ± 0.008	1.227PheTyr: 1.227 ± 0.014	0.0PheXaa: 0.0 ± 0.0
Gly	3.823GlyAla: 3.823 ± 0.028	1.066GlyCys: 1.066 ± 0.015	2.99GlyAsp: 2.99 ± 0.026	3.086GlyGlu: 3.086 ± 0.024	2.643GlyPhe: 2.643 ± 0.022	4.931GlyGly: 4.931 ± 0.071	1.501GlyHis: 1.501 ± 0.015	2.901GlyIle: 2.901 ± 0.02	3.519GlyLys: 3.519 ± 0.024	5.026GlyLeu: 5.026 ± 0.029	1.542GlyMet: 1.542 ± 0.017	2.67GlyAsn: 2.67 ± 0.025	2.77GlyPro: 2.77 ± 0.027	2.621GlyGln: 2.621 ± 0.027	3.758GlyArg: 3.758 ± 0.027	5.527GlySer: 5.527 ± 0.046	3.6GlyThr: 3.6 ± 0.029	3.517GlyVal: 3.517 ± 0.027	0.795GlyTrp: 0.795 ± 0.011	2.015GlyTyr: 2.015 ± 0.025	0.0GlyXaa: 0.0 ± 0.0
His	1.461HisAla: 1.461 ± 0.013	0.557HisCys: 0.557 ± 0.011	1.194HisAsp: 1.194 ± 0.011	1.269HisGlu: 1.269 ± 0.013	1.104HisPhe: 1.104 ± 0.012	1.555HisGly: 1.555 ± 0.015	0.91HisHis: 0.91 ± 0.014	1.153HisIle: 1.153 ± 0.012	1.064HisLys: 1.064 ± 0.013	2.395HisLeu: 2.395 ± 0.023	0.559HisMet: 0.559 ± 0.008	0.968HisAsn: 0.968 ± 0.011	1.484HisPro: 1.484 ± 0.014	1.059HisGln: 1.059 ± 0.014	1.617HisArg: 1.617 ± 0.017	2.004HisSer: 2.004 ± 0.019	1.193HisThr: 1.193 ± 0.014	1.545HisVal: 1.545 ± 0.014	0.295HisTrp: 0.295 ± 0.006	0.769HisTyr: 0.769 ± 0.009	0.0HisXaa: 0.0 ± 0.0
Ile	3.505IleAla: 3.505 ± 0.022	1.025IleCys: 1.025 ± 0.012	2.434IleAsp: 2.434 ± 0.016	2.434IleGlu: 2.434 ± 0.02	2.157IlePhe: 2.157 ± 0.02	2.63IleGly: 2.63 ± 0.018	1.162IleHis: 1.162 ± 0.013	2.35IleIle: 2.35 ± 0.02	2.328IleLys: 2.328 ± 0.018	4.566IleLeu: 4.566 ± 0.031	1.034IleMet: 1.034 ± 0.012	1.884IleAsn: 1.884 ± 0.019	2.866IlePro: 2.866 ± 0.019	1.951IleGln: 1.951 ± 0.017	2.922IleArg: 2.922 ± 0.019	3.857IleSer: 3.857 ± 0.022	2.845IleThr: 2.845 ± 0.023	3.33IleVal: 3.33 ± 0.021	0.598IleTrp: 0.598 ± 0.01	1.303IleTyr: 1.303 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	4.075LysAla: 4.075 ± 0.029	0.872LysCys: 0.872 ± 0.013	3.112LysAsp: 3.112 ± 0.022	3.934LysGlu: 3.934 ± 0.03	1.928LysPhe: 1.928 ± 0.017	2.631LysGly: 2.631 ± 0.021	1.229LysHis: 1.229 ± 0.013	2.702LysIle: 2.702 ± 0.018	4.335LysLys: 4.335 ± 0.043	4.774LysLeu: 4.774 ± 0.032	1.447LysMet: 1.447 ± 0.013	2.344LysAsn: 2.344 ± 0.018	2.888LysPro: 2.888 ± 0.029	2.167LysGln: 2.167 ± 0.02	3.679LysArg: 3.679 ± 0.023	4.015LysSer: 4.015 ± 0.028	3.292LysThr: 3.292 ± 0.023	3.317LysVal: 3.317 ± 0.027	0.64LysTrp: 0.64 ± 0.011	1.57LysTyr: 1.57 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	6.543LeuAla: 6.543 ± 0.036	1.722LeuCys: 1.722 ± 0.016	4.919LeuAsp: 4.919 ± 0.029	5.227LeuGlu: 5.227 ± 0.042	3.609LeuPhe: 3.609 ± 0.024	4.637LeuGly: 4.637 ± 0.027	2.387LeuHis: 2.387 ± 0.021	4.152LeuIle: 4.152 ± 0.031	5.012LeuLys: 5.012 ± 0.036	9.065LeuLeu: 9.065 ± 0.057	2.177LeuMet: 2.177 ± 0.018	3.62LeuAsn: 3.62 ± 0.021	5.403LeuPro: 5.403 ± 0.033	4.308LeuGln: 4.308 ± 0.028	5.853LeuArg: 5.853 ± 0.033	7.2LeuSer: 7.2 ± 0.034	5.347LeuThr: 5.347 ± 0.03	5.529LeuVal: 5.529 ± 0.031	1.073LeuTrp: 1.073 ± 0.013	2.523LeuTyr: 2.523 ± 0.019	0.0LeuXaa: 0.0 ± 0.0
Met	2.155MetAla: 2.155 ± 0.018	0.354MetCys: 0.354 ± 0.007	1.66MetAsp: 1.66 ± 0.016	1.815MetGlu: 1.815 ± 0.017	0.92MetPhe: 0.92 ± 0.014	1.349MetGly: 1.349 ± 0.015	0.503MetHis: 0.503 ± 0.008	0.994MetIle: 0.994 ± 0.012	1.371MetLys: 1.371 ± 0.013	2.041MetLeu: 2.041 ± 0.018	0.711MetMet: 0.711 ± 0.013	1.0MetAsn: 1.0 ± 0.014	1.193MetPro: 1.193 ± 0.012	0.991MetGln: 0.991 ± 0.014	1.283MetArg: 1.283 ± 0.013	1.879MetSer: 1.879 ± 0.014	1.493MetThr: 1.493 ± 0.013	1.545MetVal: 1.545 ± 0.014	0.274MetTrp: 0.274 ± 0.005	0.668MetTyr: 0.668 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.945AsnAla: 2.945 ± 0.024	0.78AsnCys: 0.78 ± 0.013	2.088AsnAsp: 2.088 ± 0.017	2.251AsnGlu: 2.251 ± 0.019	1.76AsnPhe: 1.76 ± 0.015	3.3AsnGly: 3.3 ± 0.032	0.936AsnHis: 0.936 ± 0.012	2.151AsnIle: 2.151 ± 0.016	1.859AsnLys: 1.859 ± 0.018	3.64AsnLeu: 3.64 ± 0.025	0.998AsnMet: 0.998 ± 0.012	1.781AsnAsn: 1.781 ± 0.021	2.273AsnPro: 2.273 ± 0.018	1.679AsnGln: 1.679 ± 0.019	2.341AsnArg: 2.341 ± 0.021	3.216AsnSer: 3.216 ± 0.023	2.147AsnThr: 2.147 ± 0.019	2.818AsnVal: 2.818 ± 0.019	0.483AsnTrp: 0.483 ± 0.008	1.232AsnTyr: 1.232 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	4.492ProAla: 4.492 ± 0.035	0.758ProCys: 0.758 ± 0.013	2.829ProAsp: 2.829 ± 0.021	3.496ProGlu: 3.496 ± 0.041	2.135ProPhe: 2.135 ± 0.017	3.106ProGly: 3.106 ± 0.037	1.228ProHis: 1.228 ± 0.013	2.115ProIle: 2.115 ± 0.019	2.785ProLys: 2.785 ± 0.023	4.495ProLeu: 4.495 ± 0.025	1.09ProMet: 1.09 ± 0.012	2.179ProAsn: 2.179 ± 0.02	4.304ProPro: 4.304 ± 0.051	2.196ProGln: 2.196 ± 0.026	2.914ProArg: 2.914 ± 0.024	5.599ProSer: 5.599 ± 0.044	3.594ProThr: 3.594 ± 0.037	4.046ProVal: 4.046 ± 0.035	0.564ProTrp: 0.564 ± 0.009	1.471ProTyr: 1.471 ± 0.015	0.0ProXaa: 0.0 ± 0.0
Gln	3.026GlnAla: 3.026 ± 0.025	0.757GlnCys: 0.757 ± 0.014	1.912GlnAsp: 1.912 ± 0.016	2.542GlnGlu: 2.542 ± 0.023	1.472GlnPhe: 1.472 ± 0.013	2.045GlnGly: 2.045 ± 0.022	1.141GlnHis: 1.141 ± 0.015	1.92GlnIle: 1.92 ± 0.017	2.447GlnLys: 2.447 ± 0.019	3.967GlnLeu: 3.967 ± 0.028	1.129GlnMet: 1.129 ± 0.012	1.848GlnAsn: 1.848 ± 0.017	2.549GlnPro: 2.549 ± 0.041	2.784GlnGln: 2.784 ± 0.045	2.814GlnArg: 2.814 ± 0.024	3.247GlnSer: 3.247 ± 0.024	2.361GlnThr: 2.361 ± 0.018	2.499GlnVal: 2.499 ± 0.02	0.487GlnTrp: 0.487 ± 0.008	1.271GlnTyr: 1.271 ± 0.015	0.0GlnXaa: 0.0 ± 0.0
Arg	3.713ArgAla: 3.713 ± 0.023	1.159ArgCys: 1.159 ± 0.018	3.015ArgAsp: 3.015 ± 0.02	3.438ArgGlu: 3.438 ± 0.03	2.4ArgPhe: 2.4 ± 0.018	3.086ArgGly: 3.086 ± 0.026	1.638ArgHis: 1.638 ± 0.017	2.98ArgIle: 2.98 ± 0.022	4.147ArgLys: 4.147 ± 0.028	5.602ArgLeu: 5.602 ± 0.031	1.586ArgMet: 1.586 ± 0.015	2.763ArgAsn: 2.763 ± 0.021	3.316ArgPro: 3.316 ± 0.03	2.77ArgGln: 2.77 ± 0.021	4.861ArgArg: 4.861 ± 0.038	5.027ArgSer: 5.027 ± 0.032	3.622ArgThr: 3.622 ± 0.023	3.264ArgVal: 3.264 ± 0.024	0.812ArgTrp: 0.812 ± 0.012	1.815ArgTyr: 1.815 ± 0.016	0.0ArgXaa: 0.0 ± 0.0
Ser	6.373SerAla: 6.373 ± 0.035	1.456SerCys: 1.456 ± 0.019	4.121SerAsp: 4.121 ± 0.026	4.235SerGlu: 4.235 ± 0.029	3.417SerPhe: 3.417 ± 0.022	5.465SerGly: 5.465 ± 0.04	1.947SerHis: 1.947 ± 0.019	3.565SerIle: 3.565 ± 0.022	3.944SerLys: 3.944 ± 0.028	7.262SerLeu: 7.262 ± 0.034	1.857SerMet: 1.857 ± 0.017	3.188SerAsn: 3.188 ± 0.026	4.987SerPro: 4.987 ± 0.049	3.364SerGln: 3.364 ± 0.026	5.006SerArg: 5.006 ± 0.031	9.717SerSer: 9.717 ± 0.081	5.784SerThr: 5.784 ± 0.04	5.396SerVal: 5.396 ± 0.036	0.977SerTrp: 0.977 ± 0.012	2.189SerTyr: 2.189 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	4.897ThrAla: 4.897 ± 0.029	1.105ThrCys: 1.105 ± 0.017	3.012ThrAsp: 3.012 ± 0.022	3.544ThrGlu: 3.544 ± 0.045	2.494ThrPhe: 2.494 ± 0.02	3.729ThrGly: 3.729 ± 0.026	1.181ThrHis: 1.181 ± 0.013	2.997ThrIle: 2.997 ± 0.024	3.005ThrLys: 3.005 ± 0.021	5.137ThrLeu: 5.137 ± 0.03	1.357ThrMet: 1.357 ± 0.014	2.284ThrAsn: 2.284 ± 0.021	3.428ThrPro: 3.428 ± 0.031	1.989ThrGln: 1.989 ± 0.018	2.966ThrArg: 2.966 ± 0.022	5.645ThrSer: 5.645 ± 0.038	5.032ThrThr: 5.032 ± 0.104	4.494ThrVal: 4.494 ± 0.04	0.685ThrTrp: 0.685 ± 0.01	1.547ThrTyr: 1.547 ± 0.014	0.0ThrXaa: 0.0 ± 0.0
Val	4.951ValAla: 4.951 ± 0.029	1.302ValCys: 1.302 ± 0.016	3.777ValAsp: 3.777 ± 0.024	4.13ValGlu: 4.13 ± 0.045	2.817ValPhe: 2.817 ± 0.022	3.578ValGly: 3.578 ± 0.024	1.563ValHis: 1.563 ± 0.014	3.277ValIle: 3.277 ± 0.025	3.479ValLys: 3.479 ± 0.023	6.104ValLeu: 6.104 ± 0.032	1.564ValMet: 1.564 ± 0.015	2.593ValAsn: 2.593 ± 0.021	3.753ValPro: 3.753 ± 0.027	2.756ValGln: 2.756 ± 0.022	3.848ValArg: 3.848 ± 0.025	5.095ValSer: 5.095 ± 0.03	4.067ValThr: 4.067 ± 0.043	4.922ValVal: 4.922 ± 0.043	0.776ValTrp: 0.776 ± 0.011	1.85ValTyr: 1.85 ± 0.016	0.0ValXaa: 0.0 ± 0.0
Trp	0.656TrpAla: 0.656 ± 0.009	0.239TrpCys: 0.239 ± 0.006	0.627TrpAsp: 0.627 ± 0.01	0.591TrpGlu: 0.591 ± 0.01	0.483TrpPhe: 0.483 ± 0.007	0.545TrpGly: 0.545 ± 0.01	0.274TrpHis: 0.274 ± 0.006	0.751TrpIle: 0.751 ± 0.011	0.866TrpLys: 0.866 ± 0.011	1.163TrpLeu: 1.163 ± 0.014	0.405TrpMet: 0.405 ± 0.007	0.672TrpAsn: 0.672 ± 0.01	0.508TrpPro: 0.508 ± 0.009	0.483TrpGln: 0.483 ± 0.008	0.78TrpArg: 0.78 ± 0.01	0.976TrpSer: 0.976 ± 0.012	0.885TrpThr: 0.885 ± 0.011	0.607TrpVal: 0.607 ± 0.009	0.199TrpTrp: 0.199 ± 0.005	0.378TrpTyr: 0.378 ± 0.008	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.924TyrAla: 1.924 ± 0.016	0.648TyrCys: 0.648 ± 0.009	1.575TyrAsp: 1.575 ± 0.015	1.567TyrGlu: 1.567 ± 0.016	1.386TyrPhe: 1.386 ± 0.014	2.258TyrGly: 2.258 ± 0.03	0.783TyrHis: 0.783 ± 0.01	1.302TyrIle: 1.302 ± 0.014	1.279TyrLys: 1.279 ± 0.014	2.631TyrLeu: 2.631 ± 0.021	0.646TyrMet: 0.646 ± 0.009	1.16TyrAsn: 1.16 ± 0.013	1.501TyrPro: 1.501 ± 0.018	1.211TyrGln: 1.211 ± 0.012	1.852TyrArg: 1.852 ± 0.016	2.224TyrSer: 2.224 ± 0.019	1.46TyrThr: 1.46 ± 0.015	1.82TyrVal: 1.82 ± 0.015	0.408TyrTrp: 0.408 ± 0.008	1.02TyrTyr: 1.02 ± 0.013	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.026XaaXaa: 0.026 ± 0.008

Statistics based on 22940 proteins (8105517 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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