Alkalinema sp. CACIAM 70d

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Leptolyngbyaceae; Alkalinema; unclassified Alkalinema

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5211 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A251WKG9|A0A251WKG9_9CYAN AAA_16 domain-containing protein OS=Alkalinema sp. CACIAM 70d OX=1934309 GN=B0A82_02705 PE=4 SV=1
MM1 pKa = 8.03WDD3 pKa = 3.58PSTNTNITLSGSFTIPAGQVTIRR26 pKa = 11.84AAFAPNGKK34 pKa = 10.19LYY36 pKa = 10.89VATNAGGIYY45 pKa = 9.74EE46 pKa = 4.3VNPTDD51 pKa = 3.92GSQISSMITLKK62 pKa = 11.02DD63 pKa = 3.38SANQPLSLAGNGDD76 pKa = 3.48MAFDD80 pKa = 4.39GNGVLYY86 pKa = 10.6LFNDD90 pKa = 3.6ANLYY94 pKa = 8.31TADD97 pKa = 4.25INTQTAYY104 pKa = 11.06LLSTTAGATFNSVAFGSGGNLLAYY128 pKa = 10.09SSSNNTVFSINTNTGIATSVGSPSLGSGIVGSDD161 pKa = 3.97FGSCAQPTPNLTVLKK176 pKa = 10.17SYY178 pKa = 11.39AKK180 pKa = 10.41VGGSAGGTILPGDD193 pKa = 3.75VVEE196 pKa = 4.38YY197 pKa = 10.07TVTITNTGTVPATNSTFSDD216 pKa = 5.2AIPAGATYY224 pKa = 9.22VTGSTQMNSTAVTDD238 pKa = 4.42GSGGTFPFQTARR250 pKa = 11.84NINSPGLINGVVGVGVGYY268 pKa = 10.33QVTLKK273 pKa = 10.77YY274 pKa = 9.98RR275 pKa = 11.84VSLNAVNPPASIVNQGTVLYY295 pKa = 10.23LGNLVPGVVSDD306 pKa = 4.85DD307 pKa = 4.07PNTVAVNDD315 pKa = 4.16ATTITYY321 pKa = 9.35PVVTLSGNVFNDD333 pKa = 3.12SDD335 pKa = 3.93GSKK338 pKa = 10.45VLNGAEE344 pKa = 4.09AGTNAGGLNAVLVNSNNQVVATTAVATNGTYY375 pKa = 10.44SFSNVAVNANYY386 pKa = 8.65TVQITTANATIGSAPPVVTLPSNWVSTGEE415 pKa = 4.08NLNGTVDD422 pKa = 3.61GTVDD426 pKa = 3.18SKK428 pKa = 11.54LAVAVTTNNVTGVNFGIEE446 pKa = 4.06QLPTAVGGTATSQTNPGGTTTVSVPSTLFTGSTDD480 pKa = 3.34PDD482 pKa = 3.67GTVASYY488 pKa = 10.86KK489 pKa = 9.24ITAFPSNATSITINGTNYY507 pKa = 9.76TSASFPGAGVTVTTAQLAGMVVDD530 pKa = 5.74PIDD533 pKa = 3.61GAVNVGILFKK543 pKa = 10.92AIDD546 pKa = 3.62NAGQEE551 pKa = 4.4SSNTATATLPFTAGNVTVSGTVFDD575 pKa = 5.26DD576 pKa = 3.94ADD578 pKa = 3.8GSKK581 pKa = 10.66LLNGTEE587 pKa = 4.01TGTNGGGLNAVLVNGSNQVVATTAVAANGTYY618 pKa = 9.82TFNSVPANANYY629 pKa = 8.58TVQITTATATVGSAPPAIALPSNWVSTGEE658 pKa = 4.09NLNGTIDD665 pKa = 3.66GTVDD669 pKa = 3.1SKK671 pKa = 11.73LSVSVTTNNITGANFGIEE689 pKa = 4.12QLPTAVGGTATSQTNPGGTTTVSVPSTLFTGSTDD723 pKa = 3.34PDD725 pKa = 3.67GTVASYY731 pKa = 10.89KK732 pKa = 9.23ITAFPTNATSITINGTNYY750 pKa = 9.76TSANFPAGGVTVTTAQLAGMVVDD773 pKa = 5.74PIDD776 pKa = 3.78GAVSVGILFKK786 pKa = 10.96AIDD789 pKa = 3.62NAGQEE794 pKa = 4.4SSNTATATLPFTAVVVNNPNLLLVKK819 pKa = 10.37RR820 pKa = 11.84ITAINGTSINFFDD833 pKa = 6.48DD834 pKa = 3.45DD835 pKa = 3.79TTSAKK840 pKa = 10.26KK841 pKa = 10.48DD842 pKa = 4.01DD843 pKa = 5.04DD844 pKa = 3.86NHH846 pKa = 6.84PYY848 pKa = 9.94WPTPLNANGSLGSTSISTFLSGKK871 pKa = 9.44IDD873 pKa = 3.32AGMVKK878 pKa = 10.28SGDD881 pKa = 3.59DD882 pKa = 3.79LEE884 pKa = 4.31YY885 pKa = 10.55TIYY888 pKa = 10.46FLSAGNVPLKK898 pKa = 10.69NVNFCDD904 pKa = 4.33LVPTNTTFQPNSFGPGQGIQLSLGGSFLNLTNVPDD939 pKa = 3.52SDD941 pKa = 3.46RR942 pKa = 11.84AQFLNPGAKK951 pKa = 10.16LPTYY955 pKa = 10.14CPSASNSTGAVLVNIVNSSFAAPDD979 pKa = 3.49NEE981 pKa = 4.8LPNATAPATPGYY993 pKa = 10.19SYY995 pKa = 11.59GFVRR999 pKa = 11.84FKK1001 pKa = 11.47AKK1003 pKa = 10.67VNN1005 pKa = 3.57

Molecular weight: 101.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A251WHR2|A0A251WHR2_9CYAN Uncharacterized protein OS=Alkalinema sp. CACIAM 70d OX=1934309 GN=B0A82_10095 PE=4 SV=1
MM1 pKa = 7.53SLPLPPKK8 pKa = 9.33SQSSPSPPTRR18 pKa = 11.84VSLPRR23 pKa = 11.84LPARR27 pKa = 11.84KK28 pKa = 9.22SLPASPLRR36 pKa = 11.84KK37 pKa = 9.58LLALLPIIALSRR49 pKa = 11.84LLPMPFIAAVPSSRR63 pKa = 11.84FKK65 pKa = 10.77FSRR68 pKa = 11.84LSPRR72 pKa = 11.84VV73 pKa = 3.27

Molecular weight: 7.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5211

0

5211

1707141

29

4319

327.6

36.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.013 ± 0.038

0.956 ± 0.012

5.216 ± 0.029

5.729 ± 0.035

3.706 ± 0.02

6.92 ± 0.038

1.951 ± 0.019

5.949 ± 0.025

3.797 ± 0.026

11.087 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.959 ± 0.015

3.673 ± 0.03

5.246 ± 0.036

5.943 ± 0.035

5.686 ± 0.031

6.319 ± 0.033

5.868 ± 0.033

6.635 ± 0.026

1.531 ± 0.014

2.814 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski