Rhizomicrobium sp. SCGC AG-212-E05

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Micropepsales; Micropepsaceae; Rhizomicrobium; unclassified Rhizomicrobium

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1220 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177QAG0|A0A177QAG0_9PROT Uncharacterized protein OS=Rhizomicrobium sp. SCGC AG-212-E05 OX=1799655 GN=AYO42_00935 PE=4 SV=1
MM1 pKa = 7.74ASLITIGICVYY12 pKa = 10.56DD13 pKa = 4.16YY14 pKa = 7.39YY15 pKa = 11.11TPEE18 pKa = 3.94TGLIGSGGVILVAGAAVLMFLAALVMARR46 pKa = 11.84MPGRR50 pKa = 11.84GSLLQTFLVVSILLDD65 pKa = 3.78IIGSSVAGYY74 pKa = 9.77FLEE77 pKa = 4.72SPVIMAAQALALVGWVINVVSNAAEE102 pKa = 3.73RR103 pKa = 11.84SVEE106 pKa = 3.87TDD108 pKa = 3.09EE109 pKa = 6.38VEE111 pKa = 5.92DD112 pKa = 3.95EE113 pKa = 4.37DD114 pKa = 4.71

Molecular weight: 11.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177Q4M8|A0A177Q4M8_9PROT Uncharacterized protein OS=Rhizomicrobium sp. SCGC AG-212-E05 OX=1799655 GN=AYO42_06595 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 10.62KK3 pKa = 8.94LTLLTALLCLGTAAPALAAWNEE25 pKa = 3.8IGTIRR30 pKa = 11.84VNPGQDD36 pKa = 2.55RR37 pKa = 11.84DD38 pKa = 3.75VRR40 pKa = 11.84NFDD43 pKa = 3.31LGGPVDD49 pKa = 4.15RR50 pKa = 11.84LQLRR54 pKa = 11.84AEE56 pKa = 4.47GADD59 pKa = 3.14IRR61 pKa = 11.84CRR63 pKa = 11.84AITARR68 pKa = 11.84FGNGRR73 pKa = 11.84SQTLYY78 pKa = 10.55QGNLTEE84 pKa = 6.19GMPQNINLPGRR95 pKa = 11.84EE96 pKa = 3.81RR97 pKa = 11.84NITRR101 pKa = 11.84LTFEE105 pKa = 5.07CGAEE109 pKa = 3.69RR110 pKa = 11.84QVGNRR115 pKa = 11.84RR116 pKa = 11.84GDD118 pKa = 3.22RR119 pKa = 11.84DD120 pKa = 3.16DD121 pKa = 3.45RR122 pKa = 11.84RR123 pKa = 11.84RR124 pKa = 11.84DD125 pKa = 3.18RR126 pKa = 11.84RR127 pKa = 11.84GAGDD131 pKa = 3.42ASIVVVADD139 pKa = 2.8VDD141 pKa = 3.94RR142 pKa = 11.84YY143 pKa = 9.49RR144 pKa = 11.84KK145 pKa = 8.14QWRR148 pKa = 11.84SNPRR152 pKa = 11.84YY153 pKa = 8.48QNEE156 pKa = 3.61WSKK159 pKa = 11.04IFNWGSDD166 pKa = 3.35MVNDD170 pKa = 3.47WRR172 pKa = 11.84LLSTVSFEE180 pKa = 3.98GRR182 pKa = 11.84RR183 pKa = 11.84DD184 pKa = 3.43SEE186 pKa = 4.08RR187 pKa = 11.84AFAGWKK193 pKa = 8.36GQNVDD198 pKa = 4.33AVALMPLDD206 pKa = 4.11ADD208 pKa = 3.83VRR210 pKa = 11.84CNRR213 pKa = 11.84VSARR217 pKa = 11.84FGNGQAQPLNVNNGDD232 pKa = 3.63YY233 pKa = 10.75LRR235 pKa = 11.84RR236 pKa = 11.84GQYY239 pKa = 10.55YY240 pKa = 10.72KK241 pKa = 10.98LDD243 pKa = 3.72LPGRR247 pKa = 11.84ARR249 pKa = 11.84DD250 pKa = 3.84LVSLDD255 pKa = 3.2MRR257 pKa = 11.84CRR259 pKa = 11.84ATNSDD264 pKa = 3.44RR265 pKa = 11.84VRR267 pKa = 11.84VQIFTSRR274 pKa = 3.06

Molecular weight: 31.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1220

0

1220

369753

52

1237

303.1

32.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.43 ± 0.084

0.856 ± 0.019

5.49 ± 0.047

5.086 ± 0.058

3.842 ± 0.045

8.712 ± 0.065

2.055 ± 0.037

5.147 ± 0.041

4.131 ± 0.056

9.9 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.581 ± 0.031

2.897 ± 0.043

5.242 ± 0.046

3.303 ± 0.039

6.702 ± 0.063

5.397 ± 0.043

5.272 ± 0.055

7.168 ± 0.052

1.352 ± 0.027

2.436 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski