Lactococcus phage 05601

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Skunavirus; Lactococcus virus 05601

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A343JNJ5|A0A343JNJ5_9CAUD Uncharacterized protein OS=Lactococcus phage 05601 OX=2029658 GN=05601_26 PE=4 SV=1
MM1 pKa = 7.31EE2 pKa = 6.01CKK4 pKa = 10.53HH5 pKa = 5.85NWITLEE11 pKa = 3.83WHH13 pKa = 7.28ADD15 pKa = 3.26GCGCCGDD22 pKa = 4.61LSAVCTICGEE32 pKa = 4.42TIHH35 pKa = 7.61DD36 pKa = 3.93YY37 pKa = 11.34LNYY40 pKa = 10.05WEE42 pKa = 4.19ITKK45 pKa = 10.84LNLDD49 pKa = 3.48EE50 pKa = 7.05DD51 pKa = 4.42EE52 pKa = 5.7DD53 pKa = 4.31EE54 pKa = 4.36EE55 pKa = 4.63

Molecular weight: 6.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A343JNI0|A0A343JNI0_9CAUD Putative major tail protein OS=Lactococcus phage 05601 OX=2029658 GN=05601_11 PE=4 SV=1
MM1 pKa = 7.34WFRR4 pKa = 11.84RR5 pKa = 11.84SGMAVSKK12 pKa = 10.77DD13 pKa = 2.69IDD15 pKa = 3.13IRR17 pKa = 11.84AIGEE21 pKa = 4.01QFNEE25 pKa = 4.24KK26 pKa = 8.69VTGRR30 pKa = 11.84MADD33 pKa = 2.9WFEE36 pKa = 5.77AEE38 pKa = 5.06LKK40 pKa = 10.81AKK42 pKa = 10.71ANTAGRR48 pKa = 11.84RR49 pKa = 11.84TRR51 pKa = 11.84IIRR54 pKa = 11.84SHH56 pKa = 4.86GHH58 pKa = 5.65SYY60 pKa = 10.06TYY62 pKa = 10.77SKK64 pKa = 10.24YY65 pKa = 10.73QNTGQLGRR73 pKa = 11.84NIKK76 pKa = 10.43QYY78 pKa = 11.47DD79 pKa = 3.42RR80 pKa = 11.84GKK82 pKa = 9.55GAKK85 pKa = 9.6VVDD88 pKa = 3.46AGRR91 pKa = 11.84RR92 pKa = 11.84SDD94 pKa = 3.63YY95 pKa = 10.96TGTGYY100 pKa = 10.6HH101 pKa = 6.1GMYY104 pKa = 10.53FLVEE108 pKa = 4.16KK109 pKa = 10.74KK110 pKa = 10.71GMQDD114 pKa = 3.28VKK116 pKa = 9.28TTLKK120 pKa = 10.68KK121 pKa = 10.0GANYY125 pKa = 9.69ANSMKK130 pKa = 10.65LL131 pKa = 3.26

Molecular weight: 14.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

9663

32

999

163.8

18.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.775 ± 0.546

0.849 ± 0.19

5.661 ± 0.304

7.648 ± 0.59

4.502 ± 0.284

6.147 ± 0.593

1.325 ± 0.173

6.747 ± 0.245

9.49 ± 0.559

8.714 ± 0.326

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.473 ± 0.146

6.644 ± 0.309

2.173 ± 0.245

3.601 ± 0.252

3.467 ± 0.253

6.302 ± 0.455

6.106 ± 0.298

6.333 ± 0.399

1.47 ± 0.152

4.574 ± 0.417
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski