Cattle blood-associated circovirus-like virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Porprismacovirus; Porprismacovirus bovas2

Average proteome isoelectric point is 8.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2L0HH22|A0A2L0HH22_9VIRU Replication-associated protein OS=Cattle blood-associated circovirus-like virus OX=2077298 PE=4 SV=1
MM1 pKa = 7.14SQSQKK6 pKa = 9.97IEE8 pKa = 4.05KK9 pKa = 9.82VFQSQARR16 pKa = 11.84RR17 pKa = 11.84FHH19 pKa = 6.42IVANEE24 pKa = 3.56KK25 pKa = 10.49SLPYY29 pKa = 10.49LNNILNFLTTKK40 pKa = 9.46PYY42 pKa = 11.07VYY44 pKa = 10.01ILCRR48 pKa = 11.84KK49 pKa = 9.54GYY51 pKa = 9.5NKK53 pKa = 10.14RR54 pKa = 11.84NCEE57 pKa = 3.7HH58 pKa = 5.95AHH60 pKa = 6.25IYY62 pKa = 10.76VIFKK66 pKa = 10.33KK67 pKa = 10.42AVRR70 pKa = 11.84LSNKK74 pKa = 7.36NTHH77 pKa = 5.8GCHH80 pKa = 5.69IAKK83 pKa = 10.09CRR85 pKa = 11.84GTTQQNIEE93 pKa = 4.59YY94 pKa = 9.57IKK96 pKa = 9.95GHH98 pKa = 6.53HH99 pKa = 6.29PMEE102 pKa = 4.42VCEE105 pKa = 4.36KK106 pKa = 11.38GEE108 pKa = 3.91IPEE111 pKa = 4.3NDD113 pKa = 3.27GDD115 pKa = 3.88IPPRR119 pKa = 11.84KK120 pKa = 8.56WDD122 pKa = 3.69GFVQQIKK129 pKa = 9.33QKK131 pKa = 10.69KK132 pKa = 7.94IDD134 pKa = 3.59KK135 pKa = 9.12FDD137 pKa = 3.63PMFARR142 pKa = 11.84YY143 pKa = 8.88EE144 pKa = 4.36GYY146 pKa = 10.61ANRR149 pKa = 11.84RR150 pKa = 11.84VAEE153 pKa = 3.95IMVEE157 pKa = 3.94EE158 pKa = 4.54LEE160 pKa = 5.51DD161 pKa = 3.95YY162 pKa = 11.19EE163 pKa = 7.06GDD165 pKa = 4.93LKK167 pKa = 11.35DD168 pKa = 3.89KK169 pKa = 11.04NCWIYY174 pKa = 11.25GEE176 pKa = 4.61PGTGKK181 pKa = 10.19SYY183 pKa = 10.56SARR186 pKa = 11.84YY187 pKa = 8.61CKK189 pKa = 9.98RR190 pKa = 11.84SKK192 pKa = 10.18IYY194 pKa = 9.41PKK196 pKa = 10.14PVSKK200 pKa = 9.54WWDD203 pKa = 3.5GYY205 pKa = 10.74KK206 pKa = 9.9GQKK209 pKa = 8.48VVVMDD214 pKa = 5.31DD215 pKa = 4.23LDD217 pKa = 4.1PDD219 pKa = 4.0RR220 pKa = 11.84AKK222 pKa = 10.46MLADD226 pKa = 4.83KK227 pKa = 10.84IKK229 pKa = 9.91IWTDD233 pKa = 3.0KK234 pKa = 10.55YY235 pKa = 10.86PFVAEE240 pKa = 4.47VKK242 pKa = 10.34SSSAVVDD249 pKa = 3.36PRR251 pKa = 11.84INFIVTSNYY260 pKa = 10.33SIEE263 pKa = 4.36DD264 pKa = 3.64CFEE267 pKa = 4.5SKK269 pKa = 10.57DD270 pKa = 3.4VEE272 pKa = 4.43AIKK275 pKa = 10.7RR276 pKa = 11.84RR277 pKa = 11.84FTVLHH282 pKa = 5.42FTGEE286 pKa = 4.39SIEE289 pKa = 4.25EE290 pKa = 4.06EE291 pKa = 4.2

Molecular weight: 34.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2L0HH22|A0A2L0HH22_9VIRU Replication-associated protein OS=Cattle blood-associated circovirus-like virus OX=2077298 PE=4 SV=1
MM1 pKa = 7.21SRR3 pKa = 11.84RR4 pKa = 11.84YY5 pKa = 8.56YY6 pKa = 10.44SRR8 pKa = 11.84RR9 pKa = 11.84TRR11 pKa = 11.84VIRR14 pKa = 11.84PKK16 pKa = 10.64KK17 pKa = 8.9KK18 pKa = 7.52WASNFRR24 pKa = 11.84QLTGLTSATVNVRR37 pKa = 11.84PKK39 pKa = 9.86SQAASTHH46 pKa = 7.08LIRR49 pKa = 11.84VWYY52 pKa = 8.28ILKK55 pKa = 10.6VKK57 pKa = 10.66GDD59 pKa = 3.42MRR61 pKa = 11.84LYY63 pKa = 8.92ITASSSIFSAQFASCVAYY81 pKa = 9.82IMYY84 pKa = 10.12LPEE87 pKa = 4.26GAAASIEE94 pKa = 4.45MIQSHH99 pKa = 6.56PEE101 pKa = 3.55WIMAWSPINVNMGTVSTSQPQDD123 pKa = 3.21QNVEE127 pKa = 4.23KK128 pKa = 10.74FSMSSRR134 pKa = 11.84LKK136 pKa = 10.69RR137 pKa = 11.84NLNSGDD143 pKa = 4.03KK144 pKa = 10.17IILVIDD150 pKa = 3.9LVFTPEE156 pKa = 3.77PTEE159 pKa = 4.06PSEE162 pKa = 4.17VPLNISYY169 pKa = 10.48QPAIGVQYY177 pKa = 7.36WTCANN182 pKa = 3.56

Molecular weight: 20.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

473

182

291

236.5

27.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.92 ± 0.97

2.114 ± 0.555

4.651 ± 1.342

6.342 ± 1.366

3.594 ± 0.463

4.44 ± 0.625

2.114 ± 0.555

8.034 ± 0.114

8.879 ± 2.152

4.651 ± 0.762

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.748 ± 0.601

4.863 ± 0.045

5.074 ± 0.531

4.017 ± 0.508

5.92 ± 0.369

8.034 ± 2.218

4.44 ± 0.878

6.977 ± 0.091

2.114 ± 0.346

5.074 ± 0.371
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski