Staphylococcus phage 66

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Rountreeviridae; Rakietenvirinae; Rosenblumvirus; Staphylococcus virus 66

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4ZE41|Q4ZE41_9CAUD ORF021 OS=Staphylococcus phage 66 OX=320832 PE=4 SV=1
MM1 pKa = 7.27VNVDD5 pKa = 3.72NAPEE9 pKa = 4.13EE10 pKa = 4.39KK11 pKa = 10.02GQSYY15 pKa = 8.28TEE17 pKa = 3.85MLQLFNKK24 pKa = 9.67LIQWNPAYY32 pKa = 9.81TFDD35 pKa = 3.95NAINLVSACQQLLLNYY51 pKa = 9.4NSSVVQFLKK60 pKa = 10.99DD61 pKa = 3.43EE62 pKa = 5.02LNNEE66 pKa = 4.34TKK68 pKa = 10.41PEE70 pKa = 4.48SILSYY75 pKa = 10.49IAGDD79 pKa = 4.29DD80 pKa = 5.37PIEE83 pKa = 3.81QWNMHH88 pKa = 5.77KK89 pKa = 10.66GFYY92 pKa = 7.63EE93 pKa = 4.27TYY95 pKa = 9.67NVYY98 pKa = 10.34VFF100 pKa = 4.47

Molecular weight: 11.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q4ZE51|Q4ZE51_9CAUD ORF038 OS=Staphylococcus phage 66 OX=320832 PE=4 SV=1
MM1 pKa = 6.31VTGRR5 pKa = 11.84MHH7 pKa = 7.68SYY9 pKa = 9.87QKK11 pKa = 10.56NIKK14 pKa = 9.02KK15 pKa = 10.2RR16 pKa = 11.84LVYY19 pKa = 10.33LYY21 pKa = 10.18SKK23 pKa = 10.85KK24 pKa = 10.46NIYY27 pKa = 7.67TNKK30 pKa = 7.7VTYY33 pKa = 9.52FLKK36 pKa = 10.67RR37 pKa = 11.84EE38 pKa = 4.17MQDD41 pKa = 2.97SVQNN45 pKa = 3.87

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27

0

27

5825

36

761

215.7

25.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.794 ± 0.427

0.704 ± 0.166

7.262 ± 0.388

6.18 ± 0.611

5.185 ± 0.329

4.687 ± 0.743

2.197 ± 0.237

7.365 ± 0.606

8.481 ± 0.318

8.275 ± 0.396

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.815 ± 0.252

8.412 ± 0.43

2.386 ± 0.285

4.189 ± 0.452

3.433 ± 0.334

6.318 ± 0.308

6.077 ± 0.343

5.253 ± 0.26

0.927 ± 0.212

6.06 ± 0.438
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski