Phascolarctobacterium succinatutens YIT 12067

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Acidaminococcales; Acidaminococcaceae; Phascolarctobacterium; Phascolarctobacterium succinatutens

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2148 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E8LH17|E8LH17_9FIRM NlpC/P60 family protein OS=Phascolarctobacterium succinatutens YIT 12067 OX=626939 GN=HMPREF9443_02175 PE=3 SV=1
MM1 pKa = 7.21FTPKK5 pKa = 10.31NIQGALEE12 pKa = 4.07EE13 pKa = 5.47LYY15 pKa = 10.79DD16 pKa = 4.61LCDD19 pKa = 3.77PDD21 pKa = 5.15YY22 pKa = 10.8MVDD25 pKa = 3.24MLVNYY30 pKa = 10.23SEE32 pKa = 4.6EE33 pKa = 4.12FDD35 pKa = 5.3DD36 pKa = 5.77ISPALLAKK44 pKa = 10.25SFQKK48 pKa = 9.71NAEE51 pKa = 4.23MISEE55 pKa = 4.04YY56 pKa = 10.38RR57 pKa = 11.84VLSSAGEE64 pKa = 4.39GIDD67 pKa = 3.7YY68 pKa = 10.26QGKK71 pKa = 9.06VLLNSRR77 pKa = 11.84AVRR80 pKa = 11.84LLSYY84 pKa = 10.75VEE86 pKa = 5.09DD87 pKa = 3.48MSGDD91 pKa = 3.5EE92 pKa = 4.42KK93 pKa = 11.44VRR95 pKa = 11.84TIQSKK100 pKa = 7.86EE101 pKa = 3.44LWLAEE106 pKa = 4.02DD107 pKa = 3.22MTFYY111 pKa = 11.0VVSCMSTITMDD122 pKa = 3.5KK123 pKa = 11.1EE124 pKa = 3.65EE125 pKa = 4.92AICLNEE131 pKa = 3.85HH132 pKa = 6.46RR133 pKa = 11.84SVVTTVEE140 pKa = 4.18CEE142 pKa = 3.67DD143 pKa = 5.83DD144 pKa = 3.43IFFDD148 pKa = 3.85MGSLICEE155 pKa = 4.64LDD157 pKa = 4.38DD158 pKa = 3.63ICLFEE163 pKa = 5.52LLADD167 pKa = 4.03VDD169 pKa = 3.72ATIYY173 pKa = 10.61EE174 pKa = 4.33LL175 pKa = 4.55

Molecular weight: 19.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E8LBU7|E8LBU7_9FIRM GTP cyclohydrolase 1 type 2 homolog OS=Phascolarctobacterium succinatutens YIT 12067 OX=626939 GN=HMPREF9443_00310 PE=3 SV=1
MM1 pKa = 7.62SVRR4 pKa = 11.84QLWEE8 pKa = 3.99GTDD11 pKa = 3.45AAGWAQKK18 pKa = 11.03LNGRR22 pKa = 11.84TKK24 pKa = 11.06LLFLFLFAILMITVDD39 pKa = 3.58NPRR42 pKa = 11.84TLFILFTFTLILHH55 pKa = 6.88LAAKK59 pKa = 9.3TSIYY63 pKa = 8.97KK64 pKa = 8.76WKK66 pKa = 10.64VMAILLILGLWGSMFSQALFFAQTPRR92 pKa = 11.84TPLFVLIAPEE102 pKa = 4.14AGFIGQLTGGLFIYY116 pKa = 10.51RR117 pKa = 11.84EE118 pKa = 4.62GILYY122 pKa = 10.18GAVQGLRR129 pKa = 11.84SASMLALGLLVCWTSDD145 pKa = 3.49PRR147 pKa = 11.84QLLQALLGWRR157 pKa = 11.84LPPQMAFMLVTAIRR171 pKa = 11.84FLPVLAAEE179 pKa = 4.22TGEE182 pKa = 4.51VITALQLRR190 pKa = 11.84SDD192 pKa = 3.93SQRR195 pKa = 11.84GRR197 pKa = 11.84TAVLRR202 pKa = 11.84HH203 pKa = 6.08LPYY206 pKa = 9.87IAKK209 pKa = 9.64PLLARR214 pKa = 11.84CLRR217 pKa = 11.84RR218 pKa = 11.84AQTLALSVVSRR229 pKa = 11.84GFFAGGAGRR238 pKa = 11.84RR239 pKa = 11.84RR240 pKa = 11.84QQWDD244 pKa = 3.11ARR246 pKa = 11.84EE247 pKa = 4.01KK248 pKa = 10.86ASCLLLFAVVVAVVGSKK265 pKa = 10.01IIYY268 pKa = 9.69LLSEE272 pKa = 3.53QGFYY276 pKa = 10.91FGVFRR281 pKa = 11.84QIYY284 pKa = 8.92DD285 pKa = 3.19WTVLYY290 pKa = 10.78LL291 pKa = 3.8

Molecular weight: 32.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2148

0

2148

628214

39

2776

292.5

32.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.587 ± 0.061

1.506 ± 0.024

5.277 ± 0.042

6.628 ± 0.058

3.936 ± 0.042

7.366 ± 0.049

1.889 ± 0.022

6.567 ± 0.049

6.539 ± 0.054

9.848 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.113 ± 0.031

4.041 ± 0.039

3.665 ± 0.031

3.58 ± 0.037

4.533 ± 0.04

5.139 ± 0.034

5.035 ± 0.033

7.323 ± 0.047

0.864 ± 0.018

3.564 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski