Pseudobacteroides cellulosolvens ATCC 35603 = DSM 2933

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Pseudobacteroides; Pseudobacteroides cellulosolvens

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5771 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L6JK86|A0A0L6JK86_9FIRM SH3 domain protein OS=Pseudobacteroides cellulosolvens ATCC 35603 = DSM 2933 OX=398512 GN=Bccel_1437 PE=4 SV=1
MM1 pKa = 7.64EE2 pKa = 5.36KK3 pKa = 10.28SCLGRR8 pKa = 11.84ALQTYY13 pKa = 9.67DD14 pKa = 3.86RR15 pKa = 11.84NNVSPKK21 pKa = 10.27SIQNKK26 pKa = 6.95TNSVEE31 pKa = 4.08EE32 pKa = 4.21VNMEE36 pKa = 4.27EE37 pKa = 4.09EE38 pKa = 4.19NKK40 pKa = 10.25LQEE43 pKa = 4.49EE44 pKa = 4.45IFPNEE49 pKa = 3.87NVVVEE54 pKa = 4.76LEE56 pKa = 4.02NSYY59 pKa = 10.68PEE61 pKa = 4.26PDD63 pKa = 2.94ISEE66 pKa = 4.24SMKK69 pKa = 11.05EE70 pKa = 3.77EE71 pKa = 3.98LQEE74 pKa = 4.09NTSEE78 pKa = 4.25STDD81 pKa = 3.69EE82 pKa = 4.26INLKK86 pKa = 9.09TFMEE90 pKa = 4.28NTMKK94 pKa = 10.61EE95 pKa = 4.11NEE97 pKa = 3.82IHH99 pKa = 5.94KK100 pKa = 9.46TNIYY104 pKa = 9.69EE105 pKa = 4.05NSKK108 pKa = 9.94KK109 pKa = 10.15IHH111 pKa = 6.01WNEE114 pKa = 3.66NMLKK118 pKa = 9.54RR119 pKa = 11.84CLGAYY124 pKa = 9.89YY125 pKa = 10.37SASQNTKK132 pKa = 8.22KK133 pKa = 8.62TRR135 pKa = 11.84KK136 pKa = 5.43TWKK139 pKa = 9.41RR140 pKa = 11.84KK141 pKa = 8.39EE142 pKa = 4.23SNICDD147 pKa = 3.48EE148 pKa = 4.46NVVSKK153 pKa = 11.23NEE155 pKa = 3.85EE156 pKa = 4.48LISSEE161 pKa = 3.78SSYY164 pKa = 11.28DD165 pKa = 3.32IVPQEE170 pKa = 3.97DD171 pKa = 3.65HH172 pKa = 6.8MLDD175 pKa = 3.44SQIYY179 pKa = 8.49TGEE182 pKa = 4.01TTEE185 pKa = 5.07KK186 pKa = 10.36VNEE189 pKa = 4.03EE190 pKa = 3.75NEE192 pKa = 4.51YY193 pKa = 10.44YY194 pKa = 10.75YY195 pKa = 10.37STGSEE200 pKa = 4.39SNNDD204 pKa = 3.13EE205 pKa = 3.9SDD207 pKa = 3.66EE208 pKa = 4.24NEE210 pKa = 3.91KK211 pKa = 11.06SEE213 pKa = 5.32GEE215 pKa = 4.02FTYY218 pKa = 10.97SSDD221 pKa = 3.56NEE223 pKa = 4.48SYY225 pKa = 10.88EE226 pKa = 3.78QDD228 pKa = 3.32SLGKK232 pKa = 9.85FEE234 pKa = 5.27KK235 pKa = 10.29ISWSIISDD243 pKa = 3.35IDD245 pKa = 3.49EE246 pKa = 4.12EE247 pKa = 5.84DD248 pKa = 3.7YY249 pKa = 11.41DD250 pKa = 5.27GEE252 pKa = 4.22MDD254 pKa = 6.15SIDD257 pKa = 4.9EE258 pKa = 5.52DD259 pKa = 3.47IQADD263 pKa = 3.82NEE265 pKa = 4.56SSNEE269 pKa = 3.75TDD271 pKa = 3.09SYY273 pKa = 11.23EE274 pKa = 4.07GRR276 pKa = 11.84SDD278 pKa = 3.55VADD281 pKa = 3.8SYY283 pKa = 12.15GGGSDD288 pKa = 5.21DD289 pKa = 4.23ADD291 pKa = 3.81SYY293 pKa = 11.7EE294 pKa = 4.68DD295 pKa = 4.41GYY297 pKa = 11.58EE298 pKa = 3.78NDD300 pKa = 4.03YY301 pKa = 11.07EE302 pKa = 4.15DD303 pKa = 5.8SYY305 pKa = 11.38VQEE308 pKa = 5.39DD309 pKa = 3.98LLKK312 pKa = 10.88DD313 pKa = 3.36KK314 pKa = 10.98YY315 pKa = 10.99HH316 pKa = 6.58NGNNGTDD323 pKa = 3.33QYY325 pKa = 11.33FSEE328 pKa = 4.76AVDD331 pKa = 4.69DD332 pKa = 4.52NFEE335 pKa = 4.03SDD337 pKa = 3.8YY338 pKa = 11.46QEE340 pKa = 4.71NEE342 pKa = 3.58VLAYY346 pKa = 10.36SDD348 pKa = 3.79EE349 pKa = 4.49NEE351 pKa = 4.31VYY353 pKa = 10.44DD354 pKa = 4.68FEE356 pKa = 5.8DD357 pKa = 3.37MRR359 pKa = 11.84PVEE362 pKa = 4.16TLDD365 pKa = 5.26LEE367 pKa = 4.49IANNTIDD374 pKa = 3.57NTNWDD379 pKa = 4.32NGWDD383 pKa = 3.66EE384 pKa = 5.25CDD386 pKa = 3.65VLNNEE391 pKa = 4.27YY392 pKa = 11.14NDD394 pKa = 3.61IEE396 pKa = 4.72TVGSFIDD403 pKa = 3.58YY404 pKa = 10.8RR405 pKa = 11.84NISEE409 pKa = 5.86DD410 pKa = 3.46YY411 pKa = 10.85DD412 pKa = 5.74DD413 pKa = 6.15YY414 pKa = 12.11DD415 pKa = 5.02FDD417 pKa = 4.38CQTQIEE423 pKa = 4.7TPEE426 pKa = 3.92YY427 pKa = 9.92MSDD430 pKa = 3.54YY431 pKa = 11.16DD432 pKa = 3.8QISYY436 pKa = 9.12GQSVEE441 pKa = 4.04CRR443 pKa = 11.84IITT446 pKa = 3.66

Molecular weight: 51.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L6JX97|A0A0L6JX97_9FIRM Sulfate-transporting ATPase OS=Pseudobacteroides cellulosolvens ATCC 35603 = DSM 2933 OX=398512 GN=Bccel_5349 PE=4 SV=1
MM1 pKa = 7.69RR2 pKa = 11.84VLIAGSRR9 pKa = 11.84FYY11 pKa = 11.09RR12 pKa = 11.84DD13 pKa = 3.31YY14 pKa = 11.49QKK16 pKa = 10.8ILAVVRR22 pKa = 11.84SLDD25 pKa = 3.25IDD27 pKa = 3.8LVIAGGCRR35 pKa = 11.84VLMLWLFVLLVSAASNILSIRR56 pKa = 11.84QIGRR60 pKa = 11.84GLAKK64 pKa = 10.7VPDD67 pKa = 4.3LSGMPP72 pKa = 3.72

Molecular weight: 7.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5771

0

5771

1943841

30

11562

336.8

37.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.962 ± 0.033

1.19 ± 0.014

5.861 ± 0.028

6.505 ± 0.039

4.366 ± 0.025

6.567 ± 0.033

1.442 ± 0.014

8.963 ± 0.039

8.161 ± 0.032

8.6 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.569 ± 0.019

6.034 ± 0.034

3.326 ± 0.025

2.668 ± 0.017

3.588 ± 0.026

7.02 ± 0.037

5.442 ± 0.053

6.52 ± 0.029

0.962 ± 0.013

4.254 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski