Brevundimonas sp. Root1279

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; Brevundimonas; unclassified Brevundimonas

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3236 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q7DY63|A0A0Q7DY63_9CAUL Aspartate carbamoyltransferase OS=Brevundimonas sp. Root1279 OX=1736443 GN=pyrB PE=3 SV=1
MM1 pKa = 7.24SAWLVPTAIQQFHH14 pKa = 6.69SNYY17 pKa = 9.38NRR19 pKa = 11.84HH20 pKa = 5.65GDD22 pKa = 3.39FDD24 pKa = 4.2RR25 pKa = 11.84AMNDD29 pKa = 3.23FVMGASDD36 pKa = 3.9MLGIDD41 pKa = 3.68AGLTTTEE48 pKa = 4.25RR49 pKa = 11.84EE50 pKa = 4.13LALSEE55 pKa = 4.31SFNPSEE61 pKa = 4.04VTIVNGVGYY70 pKa = 10.61VNDD73 pKa = 6.08GITTLYY79 pKa = 10.95DD80 pKa = 3.96DD81 pKa = 5.86DD82 pKa = 7.26GDD84 pKa = 4.45GSWDD88 pKa = 3.97AALQTANGTTYY99 pKa = 11.11EE100 pKa = 4.53FGTSGWSIFGEE111 pKa = 4.3

Molecular weight: 12.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q7ED68|A0A0Q7ED68_9CAUL Acylneuraminate cytidylyltransferase OS=Brevundimonas sp. Root1279 OX=1736443 GN=ASC65_06720 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 8.99RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.7GFRR19 pKa = 11.84SRR21 pKa = 11.84MATTNGQKK29 pKa = 9.57IVARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.77GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3236

0

3236

1015852

41

4215

313.9

33.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.727 ± 0.08

0.687 ± 0.012

6.163 ± 0.054

5.749 ± 0.044

3.495 ± 0.025

9.434 ± 0.109

1.764 ± 0.019

4.344 ± 0.029

2.694 ± 0.042

9.74 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.238 ± 0.023

2.435 ± 0.037

5.454 ± 0.044

3.068 ± 0.024

7.24 ± 0.051

5.034 ± 0.031

5.426 ± 0.039

7.72 ± 0.033

1.509 ± 0.019

2.078 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski