Streptomyces regalis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9110 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0X3US79|A0A0X3US79_9ACTN Thioredoxin domain-containing protein OS=Streptomyces regalis OX=68262 GN=ADL12_19805 PE=4 SV=1
MM1 pKa = 7.02TATRR5 pKa = 11.84GPDD8 pKa = 2.96TFTLQGNFALTEE20 pKa = 5.17DD21 pKa = 3.59ITSDD25 pKa = 5.47GEE27 pKa = 4.86DD28 pKa = 3.89DD29 pKa = 3.98DD30 pKa = 5.44CKK32 pKa = 11.06GRR34 pKa = 11.84YY35 pKa = 9.15DD36 pKa = 4.53SGYY39 pKa = 10.92DD40 pKa = 3.72DD41 pKa = 3.57IAEE44 pKa = 4.49GTSVTVYY51 pKa = 10.17GASGDD56 pKa = 3.84VVATGEE62 pKa = 4.73LGDD65 pKa = 3.98STYY68 pKa = 11.58DD69 pKa = 3.59SYY71 pKa = 11.92ICTFDD76 pKa = 3.05IAVPDD81 pKa = 3.79VPKK84 pKa = 10.93GEE86 pKa = 4.08KK87 pKa = 9.39FYY89 pKa = 11.02KK90 pKa = 10.49VEE92 pKa = 3.86VSHH95 pKa = 7.04RR96 pKa = 11.84GTVQLSAEE104 pKa = 3.95QAEE107 pKa = 4.36NGEE110 pKa = 4.58LVASLGG116 pKa = 3.65

Molecular weight: 12.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A117MRS0|A0A117MRS0_9ACTN Hydroxyacid dehydrogenase OS=Streptomyces regalis OX=68262 GN=ADL12_23840 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9110

0

9110

2933057

37

4633

322.0

34.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.171 ± 0.036

0.812 ± 0.006

6.053 ± 0.02

5.788 ± 0.026

2.764 ± 0.014

9.26 ± 0.024

2.318 ± 0.013

3.311 ± 0.015

2.342 ± 0.021

10.281 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.77 ± 0.01

1.883 ± 0.017

5.903 ± 0.024

2.917 ± 0.016

7.81 ± 0.028

5.183 ± 0.022

6.251 ± 0.024

8.409 ± 0.026

1.577 ± 0.012

2.198 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski