Erwinia phage vB_EamM_Kwan

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Wellingtonvirus; unclassified Wellingtonvirus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 285 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B2IE07|A0A1B2IE07_9CAUD Putative virion structural protein OS=Erwinia phage vB_EamM_Kwan OX=1883374 GN=KWAN_164 PE=4 SV=1
MM1 pKa = 7.28LTPVQSLLDD10 pKa = 3.6VSYY13 pKa = 11.3APAIVGMKK21 pKa = 10.35DD22 pKa = 2.95AVEE25 pKa = 4.06QFLTTVEE32 pKa = 4.54SVPFDD37 pKa = 3.04QFAYY41 pKa = 10.54LNEE44 pKa = 4.04NLEE47 pKa = 4.2VEE49 pKa = 4.16QLAICFLDD57 pKa = 3.85ADD59 pKa = 4.26VVSVVIQGGLDD70 pKa = 3.01KK71 pKa = 11.12AAIKK75 pKa = 10.77SLTNYY80 pKa = 6.75YY81 pKa = 9.28TNAVAVRR88 pKa = 11.84VYY90 pKa = 11.15YY91 pKa = 10.54SDD93 pKa = 4.99QEE95 pKa = 4.29DD96 pKa = 4.35FPKK99 pKa = 10.98GDD101 pKa = 3.5DD102 pKa = 3.33WSVEE106 pKa = 3.97GLSFLMDD113 pKa = 3.36NPAFDD118 pKa = 3.95EE119 pKa = 4.19SLPAFEE125 pKa = 5.91AEE127 pKa = 3.89QDD129 pKa = 3.87DD130 pKa = 4.33VFVTIKK136 pKa = 10.94VGDD139 pKa = 3.61KK140 pKa = 11.38AMLGTLLSDD149 pKa = 4.33LNTFSVMATGEE160 pKa = 4.21YY161 pKa = 9.14QPEE164 pKa = 4.15MEE166 pKa = 4.89NEE168 pKa = 4.17LKK170 pKa = 10.99VAMAYY175 pKa = 10.76CNGQEE180 pKa = 4.13CRR182 pKa = 11.84LLVQVYY188 pKa = 9.02HH189 pKa = 6.36NSWIKK194 pKa = 10.65PMLDD198 pKa = 2.87MGFEE202 pKa = 4.27ANRR205 pKa = 11.84WILKK209 pKa = 10.25ADD211 pKa = 4.01FNHH214 pKa = 7.06LL215 pKa = 3.55

Molecular weight: 24.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B2IE96|A0A1B2IE96_9CAUD Uncharacterized protein OS=Erwinia phage vB_EamM_Kwan OX=1883374 GN=KWAN_239 PE=4 SV=1
MM1 pKa = 7.85DD2 pKa = 5.38AKK4 pKa = 10.38ALHH7 pKa = 6.74KK8 pKa = 10.96ARR10 pKa = 11.84VDD12 pKa = 3.62KK13 pKa = 11.02LCKK16 pKa = 9.68QLPHH20 pKa = 6.04RR21 pKa = 11.84TRR23 pKa = 11.84RR24 pKa = 11.84GIEE27 pKa = 3.63IALKK31 pKa = 10.48NPTRR35 pKa = 11.84YY36 pKa = 7.61WQLMRR41 pKa = 11.84QIRR44 pKa = 11.84ACEE47 pKa = 3.86MPMVKK52 pKa = 10.29

Molecular weight: 6.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

285

0

285

77176

30

2434

270.8

30.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.324 ± 0.182

0.873 ± 0.06

6.34 ± 0.083

6.063 ± 0.157

4.13 ± 0.084

6.616 ± 0.181

2.152 ± 0.075

5.275 ± 0.096

5.158 ± 0.124

9.068 ± 0.118

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.774 ± 0.063

4.832 ± 0.086

4.382 ± 0.109

3.794 ± 0.08

5.569 ± 0.119

5.865 ± 0.109

6.319 ± 0.158

7.342 ± 0.155

1.355 ± 0.051

3.769 ± 0.094
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski