Changjiang tombus-like virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFW5|A0A1L3KFW5_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 3 OX=1922817 PE=4 SV=1
MM1 pKa = 7.81ALRR4 pKa = 11.84HH5 pKa = 5.31LRR7 pKa = 11.84RR8 pKa = 11.84WLQLDD13 pKa = 3.62YY14 pKa = 11.06TPGGGVRR21 pKa = 11.84SRR23 pKa = 11.84RR24 pKa = 11.84EE25 pKa = 3.88CEE27 pKa = 3.35AHH29 pKa = 6.84YY30 pKa = 10.94GEE32 pKa = 4.87EE33 pKa = 3.95WSILIAQEE41 pKa = 3.74RR42 pKa = 11.84MTRR45 pKa = 11.84EE46 pKa = 3.7HH47 pKa = 7.21ASNAKK52 pKa = 9.96DD53 pKa = 3.19WFLGTAPCDD62 pKa = 3.48LLLPQIVVAPQPSEE76 pKa = 4.01GNAAAPAALSRR87 pKa = 11.84SEE89 pKa = 4.57DD90 pKa = 3.47SVDD93 pKa = 3.49AVSEE97 pKa = 4.37SSDD100 pKa = 3.58SVPDD104 pKa = 3.58VPGAPTPATALLVGSIPVTCPLVTDD129 pKa = 4.51RR130 pKa = 11.84PSAVLSPPTSHH141 pKa = 6.91TGAPTPPAASASRR154 pKa = 11.84VNTLCRR160 pKa = 11.84LALGVHH166 pKa = 6.66PGDD169 pKa = 4.17LTWVPAVTSTAIQVSRR185 pKa = 11.84TLVEE189 pKa = 4.01GTRR192 pKa = 11.84RR193 pKa = 11.84ATAQARR199 pKa = 11.84GWIRR203 pKa = 11.84AAVAVAEE210 pKa = 4.83RR211 pKa = 11.84EE212 pKa = 4.34LCPPPALEE220 pKa = 4.01EE221 pKa = 4.15LKK223 pKa = 10.62PVAKK227 pKa = 10.38GPRR230 pKa = 11.84QEE232 pKa = 4.07VGEE235 pKa = 4.05AASWVNAEE243 pKa = 4.19SVAMEE248 pKa = 4.07LRR250 pKa = 11.84ALFGLLTDD258 pKa = 4.19TPVHH262 pKa = 6.51RR263 pKa = 11.84EE264 pKa = 3.42MGGRR268 pKa = 11.84VARR271 pKa = 11.84EE272 pKa = 3.67ILRR275 pKa = 11.84DD276 pKa = 3.22RR277 pKa = 11.84CRR279 pKa = 11.84CGRR282 pKa = 11.84EE283 pKa = 3.98DD284 pKa = 3.03TWYY287 pKa = 10.81LSTSAVTHH295 pKa = 6.2WLSPTLTDD303 pKa = 4.14LVLATRR309 pKa = 11.84PQGFHH314 pKa = 6.35

Molecular weight: 33.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG29|A0A1L3KG29_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 3 OX=1922817 PE=4 SV=1
MM1 pKa = 7.63ASSTNEE7 pKa = 3.43QSGEE11 pKa = 3.68NRR13 pKa = 11.84VRR15 pKa = 11.84QGRR18 pKa = 11.84TAQRR22 pKa = 11.84PTRR25 pKa = 11.84RR26 pKa = 11.84GHH28 pKa = 5.26RR29 pKa = 11.84QGARR33 pKa = 11.84GKK35 pKa = 8.98QPRR38 pKa = 11.84GTDD41 pKa = 2.87TRR43 pKa = 11.84TSQRR47 pKa = 11.84PDD49 pKa = 3.84NIHH52 pKa = 6.45SPAPTKK58 pKa = 10.38DD59 pKa = 3.25AQQGGRR65 pKa = 11.84NTAKK69 pKa = 10.26VPAHH73 pKa = 5.69SKK75 pKa = 9.56HH76 pKa = 6.11RR77 pKa = 11.84RR78 pKa = 11.84SALHH82 pKa = 5.64RR83 pKa = 11.84ASRR86 pKa = 11.84GGIHH90 pKa = 6.06TPRR93 pKa = 11.84PRR95 pKa = 11.84QRR97 pKa = 11.84SGGSRR102 pKa = 11.84AVGSRR107 pKa = 11.84HH108 pKa = 6.48APSQPPGGYY117 pKa = 9.23QPSASQVEE125 pKa = 4.51RR126 pKa = 11.84WAKK129 pKa = 10.08IDD131 pKa = 3.62SLLPALLDD139 pKa = 3.72TIEE142 pKa = 4.1RR143 pKa = 11.84HH144 pKa = 5.15EE145 pKa = 4.33LRR147 pKa = 11.84PSEE150 pKa = 4.13VFQRR154 pKa = 11.84GLRR157 pKa = 11.84VARR160 pKa = 11.84NAVRR164 pKa = 11.84ARR166 pKa = 11.84RR167 pKa = 11.84QPVQPVPSVVTPDD180 pKa = 3.48RR181 pKa = 11.84GGEE184 pKa = 4.04PQLPAPPPTNPAGLPHH200 pKa = 7.27DD201 pKa = 5.08GEE203 pKa = 5.02GCTTGDD209 pKa = 3.48CNAAVLLSRR218 pKa = 11.84GNVKK222 pKa = 9.66PVCHH226 pKa = 5.59AHH228 pKa = 6.22KK229 pKa = 10.1WDD231 pKa = 3.58VRR233 pKa = 11.84QLQHH237 pKa = 6.94KK238 pKa = 9.5

Molecular weight: 25.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1313

226

535

328.3

36.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.606 ± 1.654

1.523 ± 0.31

4.57 ± 0.41

5.179 ± 0.633

2.894 ± 1.142

6.702 ± 0.778

2.818 ± 0.589

3.046 ± 0.579

3.656 ± 1.325

8.911 ± 1.293

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.133 ± 0.676

3.58 ± 0.87

8.073 ± 1.139

3.503 ± 1.043

8.835 ± 1.443

7.312 ± 0.381

6.778 ± 0.497

7.845 ± 0.611

1.752 ± 0.366

2.285 ± 0.828
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski