Amino acid dipepetide frequency for Methanosarcinales archaeon

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.756AlaAla: 20.756 ± 0.458
3.437AlaCys: 3.437 ± 0.161
6.132AlaAsp: 6.132 ± 0.166
5.13AlaGlu: 5.13 ± 0.163
3.367AlaPhe: 3.367 ± 0.091
10.029AlaGly: 10.029 ± 0.267
3.767AlaHis: 3.767 ± 0.13
3.44AlaIle: 3.44 ± 0.119
2.724AlaLys: 2.724 ± 0.11
11.84AlaLeu: 11.84 ± 0.234
2.651AlaMet: 2.651 ± 0.1
3.025AlaAsn: 3.025 ± 0.136
7.055AlaPro: 7.055 ± 0.2
4.242AlaGln: 4.242 ± 0.144
8.482AlaArg: 8.482 ± 0.195
11.846AlaSer: 11.846 ± 0.263
8.662AlaThr: 8.662 ± 0.199
9.76AlaVal: 9.76 ± 0.196
1.674AlaTrp: 1.674 ± 0.078
2.175AlaTyr: 2.175 ± 0.082
0.003AlaXaa: 0.003 ± 0.003
Cys
3.288CysAla: 3.288 ± 0.112
0.745CysCys: 0.745 ± 0.074
0.951CysAsp: 0.951 ± 0.059
0.84CysGlu: 0.84 ± 0.057
0.625CysPhe: 0.625 ± 0.041
1.386CysGly: 1.386 ± 0.077
0.488CysHis: 0.488 ± 0.039
0.758CysIle: 0.758 ± 0.05
0.479CysLys: 0.479 ± 0.038
1.658CysLeu: 1.658 ± 0.076
0.736CysMet: 0.736 ± 0.046
0.568CysAsn: 0.568 ± 0.038
0.916CysPro: 0.916 ± 0.064
0.495CysGln: 0.495 ± 0.037
1.3CysArg: 1.3 ± 0.068
1.855CysSer: 1.855 ± 0.089
1.826CysThr: 1.826 ± 0.076
2.514CysVal: 2.514 ± 0.109
0.317CysTrp: 0.317 ± 0.039
0.304CysTyr: 0.304 ± 0.032
0.0CysXaa: 0.0 ± 0.0
Asp
7.914AspAla: 7.914 ± 0.188
0.72AspCys: 0.72 ± 0.055
3.028AspAsp: 3.028 ± 0.12
3.104AspGlu: 3.104 ± 0.12
1.589AspPhe: 1.589 ± 0.078
4.658AspGly: 4.658 ± 0.172
0.878AspHis: 0.878 ± 0.06
1.677AspIle: 1.677 ± 0.079
1.322AspLys: 1.322 ± 0.085
3.244AspLeu: 3.244 ± 0.109
1.265AspMet: 1.265 ± 0.064
1.459AspAsn: 1.459 ± 0.071
1.731AspPro: 1.731 ± 0.094
0.977AspGln: 0.977 ± 0.047
2.01AspArg: 2.01 ± 0.086
3.164AspSer: 3.164 ± 0.104
3.13AspThr: 3.13 ± 0.092
4.461AspVal: 4.461 ± 0.118
0.555AspTrp: 0.555 ± 0.047
0.821AspTyr: 0.821 ± 0.055
0.0AspXaa: 0.0 ± 0.0
Glu
5.539GluAla: 5.539 ± 0.198
0.726GluCys: 0.726 ± 0.055
2.162GluAsp: 2.162 ± 0.117
2.784GluGlu: 2.784 ± 0.134
1.284GluPhe: 1.284 ± 0.069
2.835GluGly: 2.835 ± 0.113
1.401GluHis: 1.401 ± 0.068
1.237GluIle: 1.237 ± 0.07
1.579GluLys: 1.579 ± 0.105
4.287GluLeu: 4.287 ± 0.144
1.024GluMet: 1.024 ± 0.052
1.078GluAsn: 1.078 ± 0.076
1.896GluPro: 1.896 ± 0.088
1.795GluGln: 1.795 ± 0.081
3.339GluArg: 3.339 ± 0.12
2.844GluSer: 2.844 ± 0.105
2.296GluThr: 2.296 ± 0.088
3.12GluVal: 3.12 ± 0.11
0.713GluTrp: 0.713 ± 0.054
0.989GluTyr: 0.989 ± 0.058
0.0GluXaa: 0.0 ± 0.0
Phe
3.481PheAla: 3.481 ± 0.116
0.558PheCys: 0.558 ± 0.047
1.744PheAsp: 1.744 ± 0.08
1.468PheGlu: 1.468 ± 0.081
1.17PhePhe: 1.17 ± 0.073
2.223PheGly: 2.223 ± 0.093
0.72PheHis: 0.72 ± 0.047
1.389PheIle: 1.389 ± 0.07
0.954PheLys: 0.954 ± 0.06
2.549PheLeu: 2.549 ± 0.102
0.847PheMet: 0.847 ± 0.054
1.148PheAsn: 1.148 ± 0.062
1.579PhePro: 1.579 ± 0.081
0.967PheGln: 0.967 ± 0.065
1.471PheArg: 1.471 ± 0.077
2.67PheSer: 2.67 ± 0.103
2.064PheThr: 2.064 ± 0.071
2.828PheVal: 2.828 ± 0.104
0.39PheTrp: 0.39 ± 0.035
0.774PheTyr: 0.774 ± 0.052
0.0PheXaa: 0.0 ± 0.0
Gly
9.094GlyAla: 9.094 ± 0.209
1.271GlyCys: 1.271 ± 0.058
3.84GlyAsp: 3.84 ± 0.156
2.708GlyGlu: 2.708 ± 0.107
2.137GlyPhe: 2.137 ± 0.089
6.944GlyGly: 6.944 ± 0.273
1.516GlyHis: 1.516 ± 0.088
2.841GlyIle: 2.841 ± 0.12
1.852GlyLys: 1.852 ± 0.105
4.464GlyLeu: 4.464 ± 0.129
2.013GlyMet: 2.013 ± 0.086
2.391GlyAsn: 2.391 ± 0.119
2.223GlyPro: 2.223 ± 0.097
1.655GlyGln: 1.655 ± 0.073
3.773GlyArg: 3.773 ± 0.136
6.081GlySer: 6.081 ± 0.201
5.118GlyThr: 5.118 ± 0.193
5.993GlyVal: 5.993 ± 0.188
0.977GlyTrp: 0.977 ± 0.058
1.595GlyTyr: 1.595 ± 0.083
0.0GlyXaa: 0.0 ± 0.0
His
4.258HisAla: 4.258 ± 0.126
0.634HisCys: 0.634 ± 0.049
1.401HisAsp: 1.401 ± 0.065
1.351HisGlu: 1.351 ± 0.072
0.881HisPhe: 0.881 ± 0.058
1.814HisGly: 1.814 ± 0.08
0.764HisHis: 0.764 ± 0.062
1.107HisIle: 1.107 ± 0.065
0.688HisLys: 0.688 ± 0.05
2.112HisLeu: 2.112 ± 0.097
0.761HisMet: 0.761 ± 0.049
0.793HisAsn: 0.793 ± 0.056
1.522HisPro: 1.522 ± 0.074
0.65HisGln: 0.65 ± 0.045
1.88HisArg: 1.88 ± 0.081
1.852HisSer: 1.852 ± 0.084
2.042HisThr: 2.042 ± 0.091
2.92HisVal: 2.92 ± 0.109
0.304HisTrp: 0.304 ± 0.029
0.51HisTyr: 0.51 ± 0.042
0.0HisXaa: 0.0 ± 0.0
Ile
4.772IleAla: 4.772 ± 0.143
0.428IleCys: 0.428 ± 0.035
1.804IleAsp: 1.804 ± 0.075
1.509IleGlu: 1.509 ± 0.08
1.107IlePhe: 1.107 ± 0.065
1.909IleGly: 1.909 ± 0.081
0.751IleHis: 0.751 ± 0.055
1.608IleIle: 1.608 ± 0.085
0.951IleLys: 0.951 ± 0.07
2.679IleLeu: 2.679 ± 0.087
0.948IleMet: 0.948 ± 0.05
1.151IleAsn: 1.151 ± 0.061
1.912IlePro: 1.912 ± 0.083
1.116IleGln: 1.116 ± 0.081
2.438IleArg: 2.438 ± 0.109
2.689IleSer: 2.689 ± 0.098
2.559IleThr: 2.559 ± 0.102
2.996IleVal: 2.996 ± 0.1
0.387IleTrp: 0.387 ± 0.034
0.628IleTyr: 0.628 ± 0.054
0.0IleXaa: 0.0 ± 0.0
Lys
2.543LysAla: 2.543 ± 0.108
0.46LysCys: 0.46 ± 0.037
1.183LysAsp: 1.183 ± 0.078
1.344LysGlu: 1.344 ± 0.078
0.907LysPhe: 0.907 ± 0.058
1.42LysGly: 1.42 ± 0.074
0.707LysHis: 0.707 ± 0.056
0.85LysIle: 0.85 ± 0.064
1.183LysLys: 1.183 ± 0.082
2.787LysLeu: 2.787 ± 0.125
0.666LysMet: 0.666 ± 0.049
0.802LysAsn: 0.802 ± 0.055
1.512LysPro: 1.512 ± 0.077
1.084LysGln: 1.084 ± 0.06
2.086LysArg: 2.086 ± 0.095
1.763LysSer: 1.763 ± 0.092
1.449LysThr: 1.449 ± 0.092
1.782LysVal: 1.782 ± 0.075
0.444LysTrp: 0.444 ± 0.038
0.736LysTyr: 0.736 ± 0.058
0.0LysXaa: 0.0 ± 0.0
Leu
10.112LeuAla: 10.112 ± 0.244
2.08LeuCys: 2.08 ± 0.087
4.141LeuAsp: 4.141 ± 0.128
3.792LeuGlu: 3.792 ± 0.164
2.987LeuPhe: 2.987 ± 0.106
4.671LeuGly: 4.671 ± 0.119
3.51LeuHis: 3.51 ± 0.145
2.689LeuIle: 2.689 ± 0.106
2.454LeuLys: 2.454 ± 0.119
10.384LeuLeu: 10.384 ± 0.253
1.963LeuMet: 1.963 ± 0.09
2.641LeuAsn: 2.641 ± 0.1
6.335LeuPro: 6.335 ± 0.186
4.477LeuGln: 4.477 ± 0.141
6.804LeuArg: 6.804 ± 0.154
7.426LeuSer: 7.426 ± 0.154
5.508LeuThr: 5.508 ± 0.14
6.361LeuVal: 6.361 ± 0.143
1.287LeuTrp: 1.287 ± 0.07
2.147LeuTyr: 2.147 ± 0.101
0.0LeuXaa: 0.0 ± 0.0
Met
2.337MetAla: 2.337 ± 0.086
0.621MetCys: 0.621 ± 0.045
1.157MetAsp: 1.157 ± 0.068
1.135MetGlu: 1.135 ± 0.067
0.732MetPhe: 0.732 ± 0.047
1.338MetGly: 1.338 ± 0.063
0.815MetHis: 0.815 ± 0.06
0.58MetIle: 0.58 ± 0.043
0.761MetLys: 0.761 ± 0.044
2.939MetLeu: 2.939 ± 0.093
0.644MetMet: 0.644 ± 0.05
0.812MetAsn: 0.812 ± 0.045
1.579MetPro: 1.579 ± 0.077
1.23MetGln: 1.23 ± 0.062
2.134MetArg: 2.134 ± 0.09
2.109MetSer: 2.109 ± 0.087
1.367MetThr: 1.367 ± 0.061
1.497MetVal: 1.497 ± 0.068
0.491MetTrp: 0.491 ± 0.042
0.726MetTyr: 0.726 ± 0.053
0.0MetXaa: 0.0 ± 0.0
Asn
3.821AsnAla: 3.821 ± 0.122
0.498AsnCys: 0.498 ± 0.037
1.408AsnAsp: 1.408 ± 0.066
1.417AsnGlu: 1.417 ± 0.074
0.907AsnPhe: 0.907 ± 0.059
2.426AsnGly: 2.426 ± 0.124
0.796AsnHis: 0.796 ± 0.063
1.237AsnIle: 1.237 ± 0.059
0.992AsnLys: 0.992 ± 0.065
2.147AsnLeu: 2.147 ± 0.096
0.888AsnMet: 0.888 ± 0.05
1.16AsnAsn: 1.16 ± 0.073
1.471AsnPro: 1.471 ± 0.074
0.736AsnGln: 0.736 ± 0.043
1.503AsnArg: 1.503 ± 0.08
2.254AsnSer: 2.254 ± 0.102
1.88AsnThr: 1.88 ± 0.085
2.759AsnVal: 2.759 ± 0.103
0.247AsnTrp: 0.247 ± 0.027
0.621AsnTyr: 0.621 ± 0.044
0.0AsnXaa: 0.0 ± 0.0
Pro
7.423ProAla: 7.423 ± 0.216
1.148ProCys: 1.148 ± 0.063
2.264ProAsp: 2.264 ± 0.095
2.074ProGlu: 2.074 ± 0.093
1.719ProPhe: 1.719 ± 0.077
3.107ProGly: 3.107 ± 0.111
1.658ProHis: 1.658 ± 0.087
1.646ProIle: 1.646 ± 0.077
1.03ProLys: 1.03 ± 0.065
5.682ProLeu: 5.682 ± 0.177
1.129ProMet: 1.129 ± 0.063
1.335ProAsn: 1.335 ± 0.071
4.902ProPro: 4.902 ± 0.174
1.636ProGln: 1.636 ± 0.076
3.39ProArg: 3.39 ± 0.119
6.031ProSer: 6.031 ± 0.224
4.246ProThr: 4.246 ± 0.152
4.141ProVal: 4.141 ± 0.125
0.688ProTrp: 0.688 ± 0.047
1.015ProTyr: 1.015 ± 0.053
0.0ProXaa: 0.0 ± 0.0
Gln
3.65GlnAla: 3.65 ± 0.119
0.707GlnCys: 0.707 ± 0.048
1.211GlnAsp: 1.211 ± 0.071
1.379GlnGlu: 1.379 ± 0.071
1.078GlnPhe: 1.078 ± 0.07
1.693GlnGly: 1.693 ± 0.073
1.256GlnHis: 1.256 ± 0.069
0.983GlnIle: 0.983 ± 0.062
0.732GlnLys: 0.732 ± 0.051
4.395GlnLeu: 4.395 ± 0.134
0.755GlnMet: 0.755 ± 0.048
0.777GlnAsn: 0.777 ± 0.049
2.283GlnPro: 2.283 ± 0.098
1.836GlnGln: 1.836 ± 0.106
2.873GlnArg: 2.873 ± 0.106
2.432GlnSer: 2.432 ± 0.091
1.785GlnThr: 1.785 ± 0.086
2.663GlnVal: 2.663 ± 0.097
0.457GlnTrp: 0.457 ± 0.036
0.828GlnTyr: 0.828 ± 0.053
0.0GlnXaa: 0.0 ± 0.0
Arg
8.472ArgAla: 8.472 ± 0.207
1.69ArgCys: 1.69 ± 0.083
2.774ArgAsp: 2.774 ± 0.098
2.797ArgGlu: 2.797 ± 0.119
2.128ArgPhe: 2.128 ± 0.083
4.033ArgGly: 4.033 ± 0.124
1.757ArgHis: 1.757 ± 0.082
2.781ArgIle: 2.781 ± 0.114
1.725ArgLys: 1.725 ± 0.09
5.841ArgLeu: 5.841 ± 0.141
2.01ArgMet: 2.01 ± 0.077
1.703ArgAsn: 1.703 ± 0.07
2.873ArgPro: 2.873 ± 0.101
2.09ArgGln: 2.09 ± 0.089
5.295ArgArg: 5.295 ± 0.174
5.032ArgSer: 5.032 ± 0.139
4.242ArgThr: 4.242 ± 0.121
6.126ArgVal: 6.126 ± 0.151
1.015ArgTrp: 1.015 ± 0.063
1.436ArgTyr: 1.436 ± 0.064
0.0ArgXaa: 0.0 ± 0.0
Ser
11.009SerAla: 11.009 ± 0.263
1.798SerCys: 1.798 ± 0.086
3.611SerAsp: 3.611 ± 0.101
2.879SerGlu: 2.879 ± 0.102
2.499SerPhe: 2.499 ± 0.097
6.589SerGly: 6.589 ± 0.245
1.969SerHis: 1.969 ± 0.088
3.019SerIle: 3.019 ± 0.106
1.893SerLys: 1.893 ± 0.084
7.439SerLeu: 7.439 ± 0.212
2.318SerMet: 2.318 ± 0.09
2.432SerAsn: 2.432 ± 0.089
5.203SerPro: 5.203 ± 0.174
2.362SerGln: 2.362 ± 0.099
4.525SerArg: 4.525 ± 0.142
9.775SerSer: 9.775 ± 0.334
6.722SerThr: 6.722 ± 0.182
6.982SerVal: 6.982 ± 0.206
1.024SerTrp: 1.024 ± 0.058
1.608SerTyr: 1.608 ± 0.079
0.0SerXaa: 0.0 ± 0.0
Thr
8.52ThrAla: 8.52 ± 0.215
1.56ThrCys: 1.56 ± 0.085
2.822ThrAsp: 2.822 ± 0.108
2.305ThrGlu: 2.305 ± 0.093
2.131ThrPhe: 2.131 ± 0.093
4.458ThrGly: 4.458 ± 0.163
1.776ThrHis: 1.776 ± 0.081
2.416ThrIle: 2.416 ± 0.101
1.474ThrLys: 1.474 ± 0.065
6.519ThrLeu: 6.519 ± 0.148
1.471ThrMet: 1.471 ± 0.065
1.883ThrAsn: 1.883 ± 0.081
5.124ThrPro: 5.124 ± 0.187
1.944ThrGln: 1.944 ± 0.089
3.868ThrArg: 3.868 ± 0.111
6.307ThrSer: 6.307 ± 0.21
4.62ThrThr: 4.62 ± 0.161
5.121ThrVal: 5.121 ± 0.176
0.84ThrTrp: 0.84 ± 0.052
1.351ThrTyr: 1.351 ± 0.084
0.0ThrXaa: 0.0 ± 0.0
Val
9.763ValAla: 9.763 ± 0.216
2.22ValCys: 2.22 ± 0.094
4.464ValAsp: 4.464 ± 0.143
3.431ValGlu: 3.431 ± 0.114
2.258ValPhe: 2.258 ± 0.09
4.734ValGly: 4.734 ± 0.144
2.965ValHis: 2.965 ± 0.106
2.381ValIle: 2.381 ± 0.095
1.814ValLys: 1.814 ± 0.086
7.654ValLeu: 7.654 ± 0.166
1.864ValMet: 1.864 ± 0.076
2.426ValAsn: 2.426 ± 0.096
4.525ValPro: 4.525 ± 0.126
3.218ValGln: 3.218 ± 0.104
6.291ValArg: 6.291 ± 0.137
6.836ValSer: 6.836 ± 0.221
4.867ValThr: 4.867 ± 0.151
5.977ValVal: 5.977 ± 0.181
1.097ValTrp: 1.097 ± 0.061
1.868ValTyr: 1.868 ± 0.081
0.0ValXaa: 0.0 ± 0.0
Trp
1.053TrpAla: 1.053 ± 0.065
0.349TrpCys: 0.349 ± 0.035
0.491TrpAsp: 0.491 ± 0.047
0.536TrpGlu: 0.536 ± 0.044
0.495TrpPhe: 0.495 ± 0.041
0.802TrpGly: 0.802 ± 0.054
0.377TrpHis: 0.377 ± 0.034
0.501TrpIle: 0.501 ± 0.048
0.549TrpLys: 0.549 ± 0.039
1.455TrpLeu: 1.455 ± 0.078
0.488TrpMet: 0.488 ± 0.042
0.491TrpAsn: 0.491 ± 0.042
0.644TrpPro: 0.644 ± 0.052
0.66TrpGln: 0.66 ± 0.042
1.154TrpArg: 1.154 ± 0.063
1.129TrpSer: 1.129 ± 0.067
0.837TrpThr: 0.837 ± 0.052
0.843TrpVal: 0.843 ± 0.048
0.289TrpTrp: 0.289 ± 0.03
0.419TrpTyr: 0.419 ± 0.035
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.394TyrAla: 2.394 ± 0.091
0.4TyrCys: 0.4 ± 0.038
1.116TyrAsp: 1.116 ± 0.064
0.945TyrGlu: 0.945 ± 0.055
0.996TyrPhe: 0.996 ± 0.065
1.417TyrGly: 1.417 ± 0.085
0.371TyrHis: 0.371 ± 0.037
1.205TyrIle: 1.205 ± 0.073
0.552TyrLys: 0.552 ± 0.045
1.693TyrLeu: 1.693 ± 0.07
0.561TyrMet: 0.561 ± 0.044
1.094TyrAsn: 1.094 ± 0.084
0.977TyrPro: 0.977 ± 0.063
0.602TyrGln: 0.602 ± 0.048
1.205TyrArg: 1.205 ± 0.066
1.56TyrSer: 1.56 ± 0.084
1.29TyrThr: 1.29 ± 0.069
1.807TyrVal: 1.807 ± 0.088
0.365TyrTrp: 0.365 ± 0.037
0.618TyrTyr: 0.618 ± 0.051
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.003XaaHis: 0.003 ± 0.003
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.003XaaXaa: 0.003 ± 0.003
Statistics based on 1193 proteins (315384 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski