Proteome-pI 2.0 - Proteome Isoelectric Point Database

Achromobacter phage vB_AxyP_19-32_Axy12

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Rothmandenesvirinae; Dongdastvirus; Achromobacter virus Axy12

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A514CUG2|A0A514CUG2_9CAUD Uncharacterized protein OS=Achromobacter phage vB_AxyP_19-32_Axy12 OX=2591043 GN=Axy12_040 PE=4 SV=1

MM1 pKa = 8.05PLMQISINVANPLKK15 pKa = 10.58RR16 pKa = 11.84LVKK19 pKa = 10.2VDD21 pKa = 3.67PDD23 pKa = 3.56GTALEE28 pKa = 4.17SGYY31 pKa = 9.36TKK33 pKa = 10.29IGTFDD38 pKa = 3.53HH39 pKa = 7.48PSTEE43 pKa = 4.75DD44 pKa = 4.5DD45 pKa = 3.93IDD47 pKa = 4.02PLGPAEE53 pKa = 3.87NHH55 pKa = 6.18VIYY58 pKa = 10.54HH59 pKa = 6.24HH60 pKa = 5.62VQEE63 pKa = 4.5ALYY66 pKa = 9.69HH67 pKa = 5.41VKK69 pKa = 10.83AGVTPSIAGFWPDD82 pKa = 4.85NITDD86 pKa = 3.82MQSVSISYY94 pKa = 7.61TAPPEE99 pKa = 3.85EE100 pKa = 4.05VVIDD104 pKa = 4.89FISLDD109 pKa = 3.74SPTVSVAVGATVQNLIFYY127 pKa = 10.76NPDD130 pKa = 3.0TATNKK135 pKa = 9.14TLTVSSSDD143 pKa = 3.42PTKK146 pKa = 10.37ATAVYY151 pKa = 9.84DD152 pKa = 4.07GPLGGDD158 pKa = 3.47SLATGVTVTGVAAGTATITVTTANGKK184 pKa = 7.27TATFDD189 pKa = 3.54VTVTAA194 pKa = 5.29

Molecular weight: 20.22 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A514CUK1|A0A514CUK1_9CAUD Uncharacterized protein OS=Achromobacter phage vB_AxyP_19-32_Axy12 OX=2591043 GN=Axy12_006 PE=4 SV=1

MM1 pKa = 7.59FFGLFKK7 pKa = 10.98LFRR10 pKa = 11.84FAFLRR15 pKa = 11.84LVAPVTEE22 pKa = 4.18ALRR25 pKa = 11.84TEE27 pKa = 4.1MHH29 pKa = 6.03MVRR32 pKa = 11.84HH33 pKa = 5.81AAFHH37 pKa = 5.74IHH39 pKa = 6.77RR40 pKa = 11.84LMLVRR45 pKa = 11.84RR46 pKa = 11.84PEE48 pKa = 3.72QDD50 pKa = 3.18DD51 pKa = 3.77YY52 pKa = 12.25GG53 pKa = 4.47

Molecular weight: 6.42 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
82	0	82	22798	37	3428	278.0	30.88

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
9.58 ± 0.646	0.741 ± 0.127	5.816 ± 0.152	6.325 ± 0.222	3.584 ± 0.187	7.294 ± 0.281	1.983 ± 0.194	5.101 ± 0.222	5.891 ± 0.213	8.382 ± 0.206

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
3.395 ± 0.14	4.654 ± 0.189	4.597 ± 0.188	4.948 ± 0.248	4.904 ± 0.189	5.593 ± 0.213	5.908 ± 0.191	6.632 ± 0.232	1.461 ± 0.192	3.211 ± 0.249

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski