Helicobacter phage 1961P

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Schmidvirus; Helicobacter virus 1961P

Average proteome isoelectric point is 7.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 33 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I6P4B6|I6P4B6_9CAUD Phage structure protein OS=Helicobacter phage 1961P OX=1154995 PE=4 SV=1
MM1 pKa = 8.15DD2 pKa = 4.72LTNLEE7 pKa = 4.28DD8 pKa = 4.3ALNNGNFKK16 pKa = 10.09EE17 pKa = 4.17QVYY20 pKa = 10.75SGLDD24 pKa = 3.16GVYY27 pKa = 10.24RR28 pKa = 11.84ISKK31 pKa = 9.68VLNQLDD37 pKa = 3.8LLKK40 pKa = 11.0NFSEE44 pKa = 4.55HH45 pKa = 6.82DD46 pKa = 3.74LEE48 pKa = 4.71IVGGNGWVFHH58 pKa = 5.97EE59 pKa = 4.54HH60 pKa = 5.51SQAIVYY66 pKa = 7.79EE67 pKa = 4.04ILKK70 pKa = 10.6

Molecular weight: 7.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I6P005|I6P005_9CAUD JHP1044-like mosaic region protein OS=Helicobacter phage 1961P OX=1154995 PE=4 SV=1
MM1 pKa = 7.53RR2 pKa = 11.84ALRR5 pKa = 11.84YY6 pKa = 9.64KK7 pKa = 10.11HH8 pKa = 6.42ANRR11 pKa = 11.84NFKK14 pKa = 10.27PKK16 pKa = 9.52VWTNSVLLEE25 pKa = 4.08SGILEE30 pKa = 3.8ILEE33 pKa = 3.89ALKK36 pKa = 10.82AIEE39 pKa = 4.09NRR41 pKa = 11.84SRR43 pKa = 11.84SQVLEE48 pKa = 3.58RR49 pKa = 11.84LIIFFIEE56 pKa = 4.24TQKK59 pKa = 11.08GQNNEE64 pKa = 4.24KK65 pKa = 9.92AWEE68 pKa = 4.02RR69 pKa = 11.84SKK71 pKa = 10.84RR72 pKa = 11.84AYY74 pKa = 9.73KK75 pKa = 9.43RR76 pKa = 11.84TLTNQTKK83 pKa = 10.26KK84 pKa = 10.8NKK86 pKa = 9.73LKK88 pKa = 10.51RR89 pKa = 11.84KK90 pKa = 7.18QLEE93 pKa = 4.25RR94 pKa = 11.84VTKK97 pKa = 9.85AQKK100 pKa = 10.3KK101 pKa = 9.53KK102 pKa = 10.41QLQANANYY110 pKa = 8.93PFSFFEE116 pKa = 4.23RR117 pKa = 11.84AA118 pKa = 3.06

Molecular weight: 14.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

33

0

33

8240

36

919

249.7

28.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.517 ± 0.343

0.752 ± 0.208

5.109 ± 0.404

8.398 ± 0.425

4.854 ± 0.327

4.235 ± 0.344

1.383 ± 0.179

7.269 ± 0.265

11.056 ± 0.495

10.364 ± 0.411

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.881 ± 0.18

8.301 ± 0.421

2.451 ± 0.226

4.575 ± 0.429

4.053 ± 0.257

6.383 ± 0.223

4.32 ± 0.283

4.017 ± 0.375

0.558 ± 0.129

3.519 ± 0.184
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski