Hubei tombus-like virus 38

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGW0|A0A1L3KGW0_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 38 OX=1923286 PE=4 SV=1
MM1 pKa = 7.7SYY3 pKa = 10.74NVSEE7 pKa = 4.41VHH9 pKa = 6.14LQPLTRR15 pKa = 11.84ARR17 pKa = 11.84VSRR20 pKa = 11.84PFKK23 pKa = 10.52SRR25 pKa = 11.84GWYY28 pKa = 10.09LMPSILIPEE37 pKa = 4.18QTAMVPMVDD46 pKa = 4.83LEE48 pKa = 4.19NATASLATRR57 pKa = 11.84VVVLEE62 pKa = 4.24PKK64 pKa = 10.61QIDD67 pKa = 3.63FNLAKK72 pKa = 10.58LPEE75 pKa = 4.12TRR77 pKa = 11.84AKK79 pKa = 10.21YY80 pKa = 10.58LRR82 pKa = 11.84TKK84 pKa = 10.39CQFEE88 pKa = 4.25TRR90 pKa = 11.84ITAAEE95 pKa = 3.69AAAIEE100 pKa = 4.45EE101 pKa = 4.49EE102 pKa = 4.71VANDD106 pKa = 3.95PDD108 pKa = 3.75RR109 pKa = 11.84QLPYY113 pKa = 10.25QGRR116 pKa = 11.84LRR118 pKa = 11.84KK119 pKa = 9.45LVAEE123 pKa = 4.93FVRR126 pKa = 11.84DD127 pKa = 3.66PRR129 pKa = 11.84TQLPTLIQPLPLEE142 pKa = 4.62EE143 pKa = 4.18YY144 pKa = 7.71VTRR147 pKa = 11.84YY148 pKa = 9.89KK149 pKa = 10.57PAEE152 pKa = 3.79QRR154 pKa = 11.84HH155 pKa = 6.04LIEE158 pKa = 4.06EE159 pKa = 4.22AAKK162 pKa = 10.49VRR164 pKa = 11.84ATFVANSSVKK174 pKa = 10.72AFIKK178 pKa = 10.65QEE180 pKa = 4.1AGPPGDD186 pKa = 4.38PRR188 pKa = 11.84NISPRR193 pKa = 11.84SPGYY197 pKa = 9.47KK198 pKa = 9.79ACLGPYY204 pKa = 9.39IAAFEE209 pKa = 4.08AAMKK213 pKa = 9.89NCSYY217 pKa = 10.58LIKK220 pKa = 10.73GLTLPEE226 pKa = 4.32RR227 pKa = 11.84NVKK230 pKa = 9.34MSSIMMGLYY239 pKa = 10.17DD240 pKa = 4.91GSVIDD245 pKa = 4.02IDD247 pKa = 4.24FSRR250 pKa = 11.84FDD252 pKa = 3.14MHH254 pKa = 6.67IQHH257 pKa = 7.18DD258 pKa = 3.97VLLYY262 pKa = 10.27IEE264 pKa = 4.91HH265 pKa = 7.05EE266 pKa = 4.6CIKK269 pKa = 10.59HH270 pKa = 5.56AFGYY274 pKa = 10.83DD275 pKa = 3.65PFLCWLLDD283 pKa = 3.57MQLVTKK289 pKa = 9.95GYY291 pKa = 8.89HH292 pKa = 4.61VCGVAYY298 pKa = 7.54HH299 pKa = 6.14TKK301 pKa = 10.41GGRR304 pKa = 11.84CSGDD308 pKa = 3.16VNTSIGNALINRR320 pKa = 11.84FCSWYY325 pKa = 9.92SARR328 pKa = 11.84TLKK331 pKa = 11.05LGDD334 pKa = 3.18NWEE337 pKa = 4.47SFHH340 pKa = 7.27EE341 pKa = 4.79GDD343 pKa = 5.3DD344 pKa = 4.3TIWWCSGLLVRR355 pKa = 11.84PNWIMYY361 pKa = 9.01VVEE364 pKa = 4.44LCHH367 pKa = 6.8LRR369 pKa = 11.84LGFPISISLNRR380 pKa = 11.84TLDD383 pKa = 3.47TVDD386 pKa = 3.5FCGRR390 pKa = 11.84VFYY393 pKa = 10.0TLQDD397 pKa = 3.42KK398 pKa = 9.0VMCMADD404 pKa = 3.54PVRR407 pKa = 11.84TLQKK411 pKa = 10.16FCITSRR417 pKa = 11.84PVQRR421 pKa = 11.84DD422 pKa = 3.4QYY424 pKa = 10.28SPTVAKK430 pKa = 10.39EE431 pKa = 3.82LLLAKK436 pKa = 10.56AMAYY440 pKa = 10.32FSTDD444 pKa = 2.45RR445 pKa = 11.84QMPIIGPLCWWIIQLLKK462 pKa = 10.69FEE464 pKa = 4.31EE465 pKa = 4.56HH466 pKa = 4.92VVPRR470 pKa = 11.84FEE472 pKa = 6.04HH473 pKa = 6.16DD474 pKa = 3.43TQFRR478 pKa = 11.84ADD480 pKa = 3.51LAGVVSKK487 pKa = 10.02YY488 pKa = 6.83EE489 pKa = 3.68WRR491 pKa = 11.84QFPTIPGEE499 pKa = 3.76ARR501 pKa = 11.84MLFFMRR507 pKa = 11.84TGIYY511 pKa = 9.61PQEE514 pKa = 3.8QVLWEE519 pKa = 4.14RR520 pKa = 11.84VVVTLPFIPEE530 pKa = 3.77VMPQLRR536 pKa = 11.84LAQIKK541 pKa = 10.77DD542 pKa = 3.86FDD544 pKa = 4.71PLRR547 pKa = 11.84HH548 pKa = 5.63MPIDD552 pKa = 3.66GYY554 pKa = 10.12MFEE557 pKa = 4.48

Molecular weight: 64.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGW0|A0A1L3KGW0_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 38 OX=1923286 PE=4 SV=1
MM1 pKa = 7.31EE2 pKa = 5.28AATLVVADD10 pKa = 4.08NQMDD14 pKa = 3.92RR15 pKa = 11.84NKK17 pKa = 9.95TYY19 pKa = 11.21ARR21 pKa = 11.84VVQILRR27 pKa = 11.84SRR29 pKa = 11.84MASRR33 pKa = 11.84QADD36 pKa = 3.52MTILSEE42 pKa = 4.19VATITLRR49 pKa = 11.84LADD52 pKa = 4.76NINRR56 pKa = 11.84DD57 pKa = 3.54AVLSGNFRR65 pKa = 11.84GTILDD70 pKa = 3.67YY71 pKa = 10.53FDD73 pKa = 3.39RR74 pKa = 11.84RR75 pKa = 11.84GWVAYY80 pKa = 9.15YY81 pKa = 10.35LIAPLGTWLPRR92 pKa = 11.84SVWGWMLSKK101 pKa = 8.33MTSRR105 pKa = 11.84EE106 pKa = 3.62HH107 pKa = 7.19RR108 pKa = 11.84FNRR111 pKa = 11.84WVWPHH116 pKa = 5.83VSATHH121 pKa = 6.92CSRR124 pKa = 11.84IMSADD129 pKa = 3.06EE130 pKa = 3.79LQRR133 pKa = 11.84IRR135 pKa = 11.84GALTAIDD142 pKa = 3.93PGARR146 pKa = 11.84QPPFQEE152 pKa = 4.49PGLVLNAIHH161 pKa = 7.11SDD163 pKa = 3.35PGTDD167 pKa = 3.17SDD169 pKa = 4.27GTHH172 pKa = 5.57GRR174 pKa = 11.84PRR176 pKa = 11.84EE177 pKa = 3.83RR178 pKa = 11.84DD179 pKa = 3.56RR180 pKa = 11.84IPSNPSSSTRR190 pKa = 11.84AEE192 pKa = 4.12TNRR195 pKa = 11.84LQSSQAAGNEE205 pKa = 4.19SEE207 pKa = 4.77VPQNEE212 pKa = 4.14MPIRR216 pKa = 11.84NPNNRR221 pKa = 11.84GRR223 pKa = 11.84GRR225 pKa = 11.84SNRR228 pKa = 11.84RR229 pKa = 11.84RR230 pKa = 11.84GRR232 pKa = 11.84QRR234 pKa = 11.84PRR236 pKa = 11.84STTALPRR243 pKa = 11.84QTSQTRR249 pKa = 11.84GRR251 pKa = 11.84IRR253 pKa = 11.84QGSPNSAPNIDD264 pKa = 3.73PATTVGRR271 pKa = 11.84VRR273 pKa = 11.84NEE275 pKa = 3.68VQTRR279 pKa = 11.84RR280 pKa = 11.84ATAPNRR286 pKa = 11.84RR287 pKa = 11.84GSKK290 pKa = 9.12GARR293 pKa = 11.84HH294 pKa = 6.05FRR296 pKa = 11.84GQLL299 pKa = 3.12

Molecular weight: 33.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1216

299

557

405.3

45.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.059 ± 0.601

1.48 ± 0.608

4.688 ± 0.21

4.359 ± 1.009

3.618 ± 0.805

6.003 ± 0.759

1.727 ± 0.428

5.345 ± 0.343

3.947 ± 0.986

8.553 ± 0.908

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.207 ± 0.239

4.03 ± 0.979

6.497 ± 0.239

4.77 ± 0.263

7.813 ± 2.596

7.73 ± 1.709

6.086 ± 0.555

7.072 ± 0.724

1.809 ± 0.147

3.207 ± 0.65
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski