Wuchereria bancrofti

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Spirurina; Spiruromorpha; Filarioidea; Onchocercidae; Wuchereria

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13000 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P7DX92|A0A3P7DX92_WUCBA RBR-type E3 ubiquitin transferase OS=Wuchereria bancrofti OX=6293 GN=WBA_LOCUS1435 PE=4 SV=1
MM1 pKa = 7.7NSLQIKK7 pKa = 9.42WSLNYY12 pKa = 10.25NAITSLIKK20 pKa = 10.5FKK22 pKa = 11.04YY23 pKa = 9.92LAFISLLYY31 pKa = 10.47RR32 pKa = 11.84FTFSSVNNDD41 pKa = 3.01VNNGGGGDD49 pKa = 5.02DD50 pKa = 6.13DD51 pKa = 6.84DD52 pKa = 7.23DD53 pKa = 7.21DD54 pKa = 6.73YY55 pKa = 12.16DD56 pKa = 6.59DD57 pKa = 6.57GDD59 pKa = 6.17DD60 pKa = 6.41DD61 pKa = 6.96DD62 pKa = 7.63DD63 pKa = 6.74DD64 pKa = 7.15DD65 pKa = 5.94NDD67 pKa = 4.68EE68 pKa = 5.45DD69 pKa = 4.97NDD71 pKa = 5.33DD72 pKa = 3.7INIMYY77 pKa = 9.97FLEE80 pKa = 4.29STLPVHH86 pKa = 6.22YY87 pKa = 9.67FYY89 pKa = 11.41LSTDD93 pKa = 2.94LRR95 pKa = 11.84FVHH98 pKa = 6.86IFSQITSIHH107 pKa = 6.0VKK109 pKa = 9.89TII111 pKa = 3.16

Molecular weight: 12.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P7E8P8|A0A3P7E8P8_WUCBA Uncharacterized protein OS=Wuchereria bancrofti OX=6293 GN=WBA_LOCUS5705 PE=3 SV=1
MM1 pKa = 6.71VRR3 pKa = 11.84AVRR6 pKa = 11.84ALRR9 pKa = 11.84RR10 pKa = 11.84PLRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84TARR18 pKa = 11.84VHH20 pKa = 6.25LAPGGLLRR28 pKa = 11.84RR29 pKa = 11.84IGAGALGWAVPLRR42 pKa = 11.84PRR44 pKa = 11.84LPRR47 pKa = 11.84RR48 pKa = 11.84LAPVRR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84ARR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 11.84ARR61 pKa = 11.84DD62 pKa = 3.33DD63 pKa = 3.43GAPRR67 pKa = 11.84DD68 pKa = 4.05RR69 pKa = 11.84ARR71 pKa = 11.84HH72 pKa = 4.93RR73 pKa = 11.84APRR76 pKa = 11.84AARR79 pKa = 11.84PGAGPGARR87 pKa = 11.84GRR89 pKa = 11.84RR90 pKa = 11.84RR91 pKa = 11.84RR92 pKa = 11.84ALRR95 pKa = 11.84DD96 pKa = 3.18PRR98 pKa = 11.84RR99 pKa = 11.84RR100 pKa = 11.84EE101 pKa = 3.74AAGDD105 pKa = 3.62APHH108 pKa = 7.57PLRR111 pKa = 11.84LPPRR115 pKa = 11.84PPAGRR120 pKa = 11.84VGQRR124 pKa = 11.84QRR126 pKa = 11.84PGAGRR131 pKa = 11.84GAQPRR136 pKa = 11.84RR137 pKa = 11.84QGRR140 pKa = 11.84GGPGRR145 pKa = 11.84RR146 pKa = 11.84VRR148 pKa = 11.84RR149 pKa = 11.84PRR151 pKa = 11.84LARR154 pKa = 11.84RR155 pKa = 11.84RR156 pKa = 11.84PTSGARR162 pKa = 11.84RR163 pKa = 11.84PRR165 pKa = 11.84DD166 pKa = 3.5GAGGPHH172 pKa = 6.95RR173 pKa = 11.84AGAAARR179 pKa = 11.84AAGAHH184 pKa = 5.04HH185 pKa = 7.44RR186 pKa = 11.84GRR188 pKa = 11.84ARR190 pKa = 11.84RR191 pKa = 11.84GPPARR196 pKa = 11.84GPGRR200 pKa = 11.84HH201 pKa = 4.94QPGPVRR207 pKa = 11.84ARR209 pKa = 11.84RR210 pKa = 11.84PRR212 pKa = 11.84HH213 pKa = 4.47QHH215 pKa = 5.9PALAPLPAALDD226 pKa = 3.43GDD228 pKa = 3.62RR229 pKa = 11.84RR230 pKa = 11.84LARR233 pKa = 11.84RR234 pKa = 11.84GAGAPRR240 pKa = 11.84PRR242 pKa = 11.84RR243 pKa = 11.84GLGAARR249 pKa = 11.84RR250 pKa = 11.84RR251 pKa = 11.84LAAHH255 pKa = 6.89LRR257 pKa = 11.84PGHH260 pKa = 6.13RR261 pKa = 11.84GRR263 pKa = 11.84RR264 pKa = 11.84GLVAGGHH271 pKa = 4.84QGRR274 pKa = 11.84RR275 pKa = 11.84TARR278 pKa = 11.84ARR280 pKa = 11.84RR281 pKa = 11.84RR282 pKa = 11.84RR283 pKa = 11.84RR284 pKa = 11.84GGGGARR290 pKa = 11.84GRR292 pKa = 11.84TPRR295 pKa = 11.84LGGRR299 pKa = 11.84RR300 pKa = 11.84RR301 pKa = 11.84PAAGGGARR309 pKa = 11.84AGRR312 pKa = 11.84AVGGTT317 pKa = 3.52

Molecular weight: 34.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13000

0

13000

4298790

29

7087

330.7

37.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.031 ± 0.022

2.218 ± 0.017

5.394 ± 0.016

6.627 ± 0.026

4.342 ± 0.018

4.914 ± 0.022

2.391 ± 0.009

6.693 ± 0.023

6.307 ± 0.023

9.404 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.544 ± 0.009

5.231 ± 0.017

4.061 ± 0.02

4.144 ± 0.018

5.687 ± 0.021

8.133 ± 0.025

5.587 ± 0.016

5.942 ± 0.019

1.1 ± 0.006

3.218 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski