Lentinula edodes negative-strand RNA virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Mymonaviridae; Lentimonavirus; Lentimonavirus lentinulae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P2VSI5|A0A4P2VSI5_9MONO Large structural protein OS=Lentinula edodes negative-strand RNA virus 1 OX=2547430 GN=L PE=4 SV=1
MM1 pKa = 7.67AFYY4 pKa = 9.77STVIGSDD11 pKa = 3.75EE12 pKa = 4.31YY13 pKa = 10.25QTSSPTPLEE22 pKa = 4.02VATADD27 pKa = 4.15DD28 pKa = 4.8LNGLSSSDD36 pKa = 3.93SFWGIIVKK44 pKa = 9.85PNQRR48 pKa = 11.84FGYY51 pKa = 9.96SIGYY55 pKa = 9.54VNGSKK60 pKa = 10.36EE61 pKa = 4.04KK62 pKa = 10.54RR63 pKa = 11.84GLAQEE68 pKa = 4.24DD69 pKa = 3.94VTRR72 pKa = 11.84SVLEE76 pKa = 3.95IPGSFAVLIRR86 pKa = 11.84GEE88 pKa = 4.16PKK90 pKa = 9.69RR91 pKa = 11.84CGAAVSQTGGSYY103 pKa = 10.33PVIEE107 pKa = 4.67FFSLSGCKK115 pKa = 9.89VSFMLPPFKK124 pKa = 10.27RR125 pKa = 11.84IYY127 pKa = 9.22TIPASFEE134 pKa = 4.07GLLTWVSDD142 pKa = 3.34INGAHH147 pKa = 6.99DD148 pKa = 3.84RR149 pKa = 11.84ALSQPGPSGSHH160 pKa = 5.42GTAEE164 pKa = 3.95WVPYY168 pKa = 9.72PNLPGLVSGPIIGARR183 pKa = 11.84AHH185 pKa = 6.78PGFEE189 pKa = 4.18TPEE192 pKa = 4.03RR193 pKa = 11.84AEE195 pKa = 4.79DD196 pKa = 3.93SLPSDD201 pKa = 4.62DD202 pKa = 5.02PPPYY206 pKa = 10.6SPP208 pKa = 5.24

Molecular weight: 22.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V0P316|A0A4V0P316_9MONO ORF5 protein OS=Lentinula edodes negative-strand RNA virus 1 OX=2547430 GN=ORF5 PE=4 SV=1
MM1 pKa = 7.0VLSRR5 pKa = 11.84SSTYY9 pKa = 10.69LPQEE13 pKa = 3.72TVARR17 pKa = 11.84IDD19 pKa = 3.41GWRR22 pKa = 11.84HH23 pKa = 3.66EE24 pKa = 4.28VARR27 pKa = 11.84WRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84GQEE34 pKa = 4.02LANMRR39 pKa = 11.84ANQRR43 pKa = 11.84CRR45 pKa = 11.84YY46 pKa = 8.89LIVVRR51 pKa = 11.84GWRR54 pKa = 11.84DD55 pKa = 2.91RR56 pKa = 11.84PFRR59 pKa = 11.84YY60 pKa = 8.59TNGFSSAILQRR71 pKa = 11.84EE72 pKa = 3.89EE73 pKa = 4.54DD74 pKa = 3.56NAVDD78 pKa = 3.95VEE80 pKa = 4.24LEE82 pKa = 4.08RR83 pKa = 11.84LSLRR87 pKa = 11.84DD88 pKa = 3.32LPGYY92 pKa = 10.13IGTLTVPGAVKK103 pKa = 8.85ITRR106 pKa = 11.84VEE108 pKa = 4.14VEE110 pKa = 4.19DD111 pKa = 3.7TTGEE115 pKa = 4.14NIVIRR120 pKa = 11.84WFQDD124 pKa = 3.31DD125 pKa = 4.64LDD127 pKa = 4.12MKK129 pKa = 10.07VMRR132 pKa = 11.84VSRR135 pKa = 11.84HH136 pKa = 5.07GFDD139 pKa = 3.46VRR141 pKa = 11.84CPAA144 pKa = 4.4

Molecular weight: 16.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3521

144

1969

503.0

56.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.725 ± 1.201

1.221 ± 0.324

5.936 ± 0.267

5.794 ± 0.972

3.295 ± 0.372

6.191 ± 0.533

2.3 ± 0.422

5.709 ± 0.621

3.948 ± 0.462

10.196 ± 0.531

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.3 ± 0.292

2.357 ± 0.523

5.737 ± 0.919

3.635 ± 0.463

7.242 ± 0.752

9.145 ± 0.693

5.794 ± 0.681

6.958 ± 0.505

1.363 ± 0.26

3.153 ± 0.645
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski