Coraliomargarita akajimensis (strain DSM 45221 / IAM 15411 / JCM 23193 / KCTC 12865 / 04OKA010-24)

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Opitutae; Puniceicoccales; Puniceicoccaceae; Coraliomargarita; Coraliomargarita akajimensis

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3110 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5EMR3|D5EMR3_CORAD 3-phosphoshikimate 1-carboxyvinyltransferase OS=Coraliomargarita akajimensis (strain DSM 45221 / IAM 15411 / JCM 23193 / KCTC 12865 / 04OKA010-24) OX=583355 GN=aroA PE=3 SV=1
MM1 pKa = 7.7KK2 pKa = 10.11KK3 pKa = 8.18QTLISMFAAAATSYY17 pKa = 11.16SFAGVAGAPNGNGLYY32 pKa = 10.23EE33 pKa = 4.01ITDD36 pKa = 4.96PISADD41 pKa = 3.4TVLTSDD47 pKa = 4.15KK48 pKa = 10.7EE49 pKa = 4.21YY50 pKa = 11.09LLTEE54 pKa = 4.04IVYY57 pKa = 10.64VSDD60 pKa = 3.2ATLTIEE66 pKa = 5.07AGTTIYY72 pKa = 10.89GVVGVNGTDD81 pKa = 3.68PEE83 pKa = 4.85SGDD86 pKa = 3.92PIALVEE92 pKa = 4.62PGTLVITRR100 pKa = 11.84TAEE103 pKa = 3.38IDD105 pKa = 3.51AVGTPTSPIIFTYY118 pKa = 10.56FGDD121 pKa = 4.02ADD123 pKa = 3.8PRR125 pKa = 11.84NTPTAPLTNDD135 pKa = 3.51TLNTGLWGGVIILGNAPTNNDD156 pKa = 2.78QSADD160 pKa = 3.52NSLLVPGTDD169 pKa = 3.45AIEE172 pKa = 5.25GIVEE176 pKa = 4.31PNPADD181 pKa = 3.9LSPDD185 pKa = 2.94LRR187 pKa = 11.84GIYY190 pKa = 10.49GGDD193 pKa = 3.57APHH196 pKa = 7.44DD197 pKa = 3.9SSGVMRR203 pKa = 11.84YY204 pKa = 9.02VSIRR208 pKa = 11.84NGGFEE213 pKa = 4.41LGAANEE219 pKa = 4.35INGLTMGGVGAGTVLEE235 pKa = 4.51YY236 pKa = 10.67IEE238 pKa = 5.39VIAGQDD244 pKa = 4.4DD245 pKa = 3.85GFEE248 pKa = 4.28WFGGTVNSKK257 pKa = 10.62YY258 pKa = 10.83LLSAYY263 pKa = 10.81NNDD266 pKa = 3.73DD267 pKa = 4.23AFDD270 pKa = 4.1YY271 pKa = 11.23DD272 pKa = 3.65QGFSGLGQFWAVVQADD288 pKa = 3.68NSNDD292 pKa = 3.14GDD294 pKa = 3.84KK295 pKa = 11.31AFEE298 pKa = 4.49FDD300 pKa = 5.52GDD302 pKa = 3.95DD303 pKa = 3.33QALAKK308 pKa = 9.91PYY310 pKa = 10.05AVPVIYY316 pKa = 10.48NATIIGTGTEE326 pKa = 4.15YY327 pKa = 11.08KK328 pKa = 10.35AGNGGGQVDD337 pKa = 3.89YY338 pKa = 11.12DD339 pKa = 3.93KK340 pKa = 11.57NAGGGLFNSIIVASGIEE357 pKa = 4.06SLDD360 pKa = 4.04LNGTCIDD367 pKa = 4.12MYY369 pKa = 11.24NAGLLQIRR377 pKa = 11.84SNIWHH382 pKa = 7.38RR383 pKa = 11.84DD384 pKa = 2.96IDD386 pKa = 4.05DD387 pKa = 4.3LAAEE391 pKa = 4.35AVVDD395 pKa = 4.39HH396 pKa = 7.35DD397 pKa = 5.57AGDD400 pKa = 3.94ADD402 pKa = 4.67VPLSDD407 pKa = 3.82AANFNVFSNPFLGGANYY424 pKa = 10.96AVMGEE429 pKa = 4.06TGTAKK434 pKa = 10.04KK435 pKa = 9.8RR436 pKa = 11.84YY437 pKa = 8.28EE438 pKa = 3.93VGGVYY443 pKa = 10.6LKK445 pKa = 10.51PAVFAGSDD453 pKa = 3.62VITNLEE459 pKa = 4.19PYY461 pKa = 9.8IGTFFDD467 pKa = 3.64VVSYY471 pKa = 10.84KK472 pKa = 10.9GAFDD476 pKa = 4.57PNGTPWTAGWTVPAQLGYY494 pKa = 10.2FADD497 pKa = 3.81

Molecular weight: 51.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5EMZ9|D5EMZ9_CORAD Putative acyl carrier protein OS=Coraliomargarita akajimensis (strain DSM 45221 / IAM 15411 / JCM 23193 / KCTC 12865 / 04OKA010-24) OX=583355 GN=Caka_2373 PE=4 SV=1
MM1 pKa = 7.59GNLKK5 pKa = 10.05KK6 pKa = 10.26KK7 pKa = 10.28RR8 pKa = 11.84RR9 pKa = 11.84LKK11 pKa = 9.46MSKK14 pKa = 9.18HH15 pKa = 5.32KK16 pKa = 10.09RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.03RR20 pKa = 11.84LKK22 pKa = 9.94SNRR25 pKa = 11.84HH26 pKa = 5.57KK27 pKa = 10.88KK28 pKa = 8.44RR29 pKa = 11.84TWW31 pKa = 2.8

Molecular weight: 4.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3110

0

3110

1118454

30

16477

359.6

39.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.406 ± 0.048

1.088 ± 0.025

5.922 ± 0.05

6.643 ± 0.047

4.121 ± 0.03

7.628 ± 0.075

2.143 ± 0.034

5.662 ± 0.037

4.194 ± 0.064

9.995 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.235 ± 0.031

3.586 ± 0.043

4.5 ± 0.043

3.905 ± 0.031

5.524 ± 0.063

6.612 ± 0.052

5.506 ± 0.086

6.776 ± 0.041

1.433 ± 0.029

3.118 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski