Ruminococcus sp. CAG:403

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; environmental samples

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1971 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7GZU1|R7GZU1_9FIRM Uncharacterized protein OS=Ruminococcus sp. CAG:403 OX=1262958 GN=BN645_01733 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.56KK3 pKa = 10.43LFACTAALALLTGACFALPASAEE26 pKa = 4.14EE27 pKa = 4.13TTYY30 pKa = 11.07RR31 pKa = 11.84PGDD34 pKa = 3.14VDD36 pKa = 3.5MDD38 pKa = 4.14GKK40 pKa = 9.03LTAMDD45 pKa = 4.01ASLILDD51 pKa = 4.37CYY53 pKa = 10.72AALSVDD59 pKa = 3.83EE60 pKa = 4.92PSPLTEE66 pKa = 4.35AQTALADD73 pKa = 3.67YY74 pKa = 10.88DD75 pKa = 4.13GDD77 pKa = 4.59GKK79 pKa = 9.39ITLQDD84 pKa = 3.91SIQVLVHH91 pKa = 6.52GDD93 pKa = 3.37YY94 pKa = 10.99LAYY97 pKa = 10.22DD98 pKa = 3.7VNLDD102 pKa = 3.7GVVDD106 pKa = 4.14AKK108 pKa = 10.91DD109 pKa = 3.33AEE111 pKa = 4.53LAAEE115 pKa = 5.19AYY117 pKa = 7.04TTFQKK122 pKa = 10.19EE123 pKa = 4.39HH124 pKa = 6.82PGEE127 pKa = 4.05ALTWKK132 pKa = 10.57NVDD135 pKa = 3.37ADD137 pKa = 4.23FMLSPLQVANFAITNVWEE155 pKa = 4.17SSNDD159 pKa = 3.48FVISPQSIRR168 pKa = 11.84WFCNSKK174 pKa = 10.93LNVPSGDD181 pKa = 3.17VDD183 pKa = 4.51LDD185 pKa = 3.94GTVSLDD191 pKa = 3.48DD192 pKa = 3.35VTAILTEE199 pKa = 4.03YY200 pKa = 9.66TQLCVEE206 pKa = 5.05KK207 pKa = 10.89DD208 pKa = 3.62PIFNSLQTILADD220 pKa = 3.72TNEE223 pKa = 4.74DD224 pKa = 3.83GVVSAGDD231 pKa = 3.35VTAALCTYY239 pKa = 9.78MDD241 pKa = 4.28GSVKK245 pKa = 9.61TAQQ248 pKa = 3.16

Molecular weight: 26.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7GZ95|R7GZ95_9FIRM Formate C-acetyltransferase glycine radical OS=Ruminococcus sp. CAG:403 OX=1262958 GN=BN645_01575 PE=4 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84SDD4 pKa = 3.25MDD6 pKa = 5.0FILLLLTRR14 pKa = 11.84NPEE17 pKa = 4.16RR18 pKa = 11.84NGNLAFRR25 pKa = 11.84VLFCLSYY32 pKa = 11.78GNFFQQNSKK41 pKa = 10.35NRR43 pKa = 11.84IRR45 pKa = 11.84GTDD48 pKa = 3.12AVFSKK53 pKa = 11.08GIFMKK58 pKa = 10.65KK59 pKa = 9.53FLRR62 pKa = 11.84KK63 pKa = 7.93DD64 pKa = 3.24TYY66 pKa = 10.19IIRR69 pKa = 11.84MM70 pKa = 3.84

Molecular weight: 8.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1971

0

1971

609732

30

2961

309.4

34.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.713 ± 0.059

1.905 ± 0.03

5.475 ± 0.045

6.473 ± 0.053

3.9 ± 0.037

6.888 ± 0.049

2.06 ± 0.029

6.581 ± 0.044

5.427 ± 0.043

9.373 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.828 ± 0.03

3.835 ± 0.041

3.76 ± 0.039

4.613 ± 0.051

4.268 ± 0.049

6.037 ± 0.052

6.328 ± 0.09

6.717 ± 0.045

0.949 ± 0.022

3.863 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski