Rhizobium tubonense

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6043 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2W4CZZ1|A0A2W4CZZ1_9RHIZ DNA repair exonuclease OS=Rhizobium tubonense OX=484088 GN=CPY51_00960 PE=4 SV=1
MM1 pKa = 7.65TDD3 pKa = 3.17DD4 pKa = 3.69HH5 pKa = 7.49SKK7 pKa = 10.61VAALADD13 pKa = 3.87GFANSAVNSTNIDD26 pKa = 3.66DD27 pKa = 4.62GSTGLVGVGNSSNSHH42 pKa = 6.29NDD44 pKa = 2.76NSTDD48 pKa = 3.39TTNTDD53 pKa = 2.9ASQTNGNGDD62 pKa = 3.62NRR64 pKa = 11.84DD65 pKa = 3.1NSYY68 pKa = 11.77DD69 pKa = 3.56FDD71 pKa = 4.74SKK73 pKa = 11.93VNTTTNDD80 pKa = 2.56ITSSYY85 pKa = 9.82NHH87 pKa = 6.61QDD89 pKa = 2.74TDD91 pKa = 3.87TKK93 pKa = 10.07VTSITDD99 pKa = 3.46TKK101 pKa = 11.21DD102 pKa = 2.96SYY104 pKa = 10.6NTSTKK109 pKa = 10.87DD110 pKa = 2.85SGNAYY115 pKa = 10.09NYY117 pKa = 10.02SDD119 pKa = 3.65SSTHH123 pKa = 6.35VKK125 pKa = 10.55DD126 pKa = 4.04SNNSLSEE133 pKa = 4.31SYY135 pKa = 10.59TKK137 pKa = 10.83DD138 pKa = 2.91SGNYY142 pKa = 9.11SSDD145 pKa = 3.16SSTHH149 pKa = 5.99SSSDD153 pKa = 3.37YY154 pKa = 11.6DD155 pKa = 4.0FGTLKK160 pKa = 10.86DD161 pKa = 3.31IGEE164 pKa = 4.18VSGNVGIAGGDD175 pKa = 3.46LAFNLGDD182 pKa = 4.18DD183 pKa = 3.85YY184 pKa = 11.92SFNLNLDD191 pKa = 4.07NILNNSLNGDD201 pKa = 3.76GNHH204 pKa = 6.68TGFSLVQANNLADD217 pKa = 3.78QDD219 pKa = 3.79TAYY222 pKa = 10.71NVSMNNAHH230 pKa = 6.63AHH232 pKa = 6.23NDD234 pKa = 3.42LSSEE238 pKa = 4.05GGDD241 pKa = 3.2AHH243 pKa = 7.52SDD245 pKa = 3.0AGIGFDD251 pKa = 5.93SKK253 pKa = 11.36LSWDD257 pKa = 3.69PSTPVAGHH265 pKa = 7.29DD266 pKa = 3.58LTGTSTADD274 pKa = 3.13SSSILANSGYY284 pKa = 9.77HH285 pKa = 5.28QEE287 pKa = 4.2LVQGANMVSNTSDD300 pKa = 2.94IAITGGDD307 pKa = 3.41HH308 pKa = 7.4HH309 pKa = 7.48DD310 pKa = 3.72VTTSSS315 pKa = 3.17

Molecular weight: 32.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2W4ELE6|A0A2W4ELE6_9RHIZ DNA-binding response regulator OS=Rhizobium tubonense OX=484088 GN=CPY51_10220 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.43LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.38GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.22GGRR28 pKa = 11.84KK29 pKa = 9.51VIIARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6043

0

6043

1816925

29

2834

300.7

32.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.555 ± 0.038

0.808 ± 0.009

5.758 ± 0.025

5.562 ± 0.031

3.967 ± 0.021

8.234 ± 0.027

2.048 ± 0.015

5.835 ± 0.025

3.813 ± 0.027

9.924 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.565 ± 0.015

2.97 ± 0.021

4.83 ± 0.022

3.152 ± 0.021

6.507 ± 0.031

6.061 ± 0.024

5.418 ± 0.021

7.373 ± 0.026

1.289 ± 0.012

2.332 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski