Candidatus Paracaedibacter acanthamoebae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales; Candidatus Paracaedibacteraceae; Candidatus Paracaedibacter

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1803 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A077ATH3|A0A077ATH3_9PROT Uncharacterized protein OS=Candidatus Paracaedibacter acanthamoebae OX=91604 GN=ID47_06520 PE=4 SV=1
MM1 pKa = 7.52TLQTLQTTLSQLNSTNISEE20 pKa = 4.58AVISNAGLTPRR31 pKa = 11.84EE32 pKa = 4.19DD33 pKa = 4.42LDD35 pKa = 4.46NLLQSALMLTQPLNVNIVNVGTADD59 pKa = 3.6DD60 pKa = 3.96TSLVIQATGSFLNLSSINLQIEE82 pKa = 4.3FVLNQDD88 pKa = 2.91ITDD91 pKa = 4.01VIINATLPDD100 pKa = 3.76NQGQAWQFADD110 pKa = 3.57SFPYY114 pKa = 8.89MVIYY118 pKa = 9.77PFYY121 pKa = 10.16NISFNSPSFIFSSYY135 pKa = 8.34GQPYY139 pKa = 8.0TWQSQPLSLTAGLNFASFLPLDD161 pKa = 3.82SGFVGLVSNFFSTPPSGEE179 pKa = 3.73ILFSGSVDD187 pKa = 3.1TSVIGKK193 pKa = 9.35NNIVWPSVNFSGLMSDD209 pKa = 3.85QPSDD213 pKa = 4.24FIPGLEE219 pKa = 4.02FTNPIITLTSQIDD232 pKa = 3.95LDD234 pKa = 4.56DD235 pKa = 4.01MQSYY239 pKa = 10.93SLNFSVTEE247 pKa = 4.0DD248 pKa = 3.43QIGLNLEE255 pKa = 4.15TQLQNNAVVGFSIVPSDD272 pKa = 3.58STNPPSLNTVVQLIPGLTAADD293 pKa = 3.59ITNDD297 pKa = 3.09IPQEE301 pKa = 3.99LQQSFSAITFVEE313 pKa = 5.06ASCTLCINPINILSMTLVLSAGSAWAITDD342 pKa = 3.43EE343 pKa = 4.45FTVKK347 pKa = 10.46SLTLSYY353 pKa = 10.49TVLDD357 pKa = 4.05PLGTPTNIFGFNGVLSFYY375 pKa = 10.73PEE377 pKa = 4.0VFKK380 pKa = 11.29GLFDD384 pKa = 3.53IQISIDD390 pKa = 3.51TTNNNLLVSGSYY402 pKa = 9.98QGIIDD407 pKa = 5.18LNTITTNLCNLSLPSQFSSINVTDD431 pKa = 4.1FVVSFNKK438 pKa = 9.96INTNWTWGLFCNVDD452 pKa = 3.32GTFPLPFVQGSVDD465 pKa = 3.9CNLSASLSSNSYY477 pKa = 9.02YY478 pKa = 10.93LEE480 pKa = 4.74GGLTIGGSYY489 pKa = 10.73FNVSFDD495 pKa = 4.3SNLKK499 pKa = 9.67NSVLAGYY506 pKa = 7.65WKK508 pKa = 10.6SLSSDD513 pKa = 3.76DD514 pKa = 4.53LLGIQSLADD523 pKa = 3.25AVGIKK528 pKa = 8.42VTIPTTFDD536 pKa = 3.86LDD538 pKa = 3.46LTQAGFSYY546 pKa = 11.26NLTQNTVKK554 pKa = 11.03LEE556 pKa = 4.07ATSLNYY562 pKa = 10.72GKK564 pKa = 9.2GTFATFLPKK573 pKa = 9.65GTADD577 pKa = 2.91QYY579 pKa = 11.78EE580 pKa = 4.96FYY582 pKa = 11.04FNLDD586 pKa = 3.24VQTDD590 pKa = 3.77IVILSQDD597 pKa = 3.55LPLIGQEE604 pKa = 4.17FSTADD609 pKa = 3.79EE610 pKa = 4.4IALKK614 pKa = 10.51SLNITYY620 pKa = 10.74ASASLPNVNPIKK632 pKa = 10.67NFNSGLDD639 pKa = 3.34IKK641 pKa = 10.99GVLSIAGDD649 pKa = 3.74DD650 pKa = 4.13HH651 pKa = 7.53PFDD654 pKa = 5.73LDD656 pKa = 4.01FGPAVQTEE664 pKa = 4.48SGGAPSLITDD674 pKa = 4.34LTATSSTPPPTPPGVWVNIQKK695 pKa = 8.61TVGPISLKK703 pKa = 10.62RR704 pKa = 11.84IGLNYY709 pKa = 10.19QNDD712 pKa = 4.45LMWIMLDD719 pKa = 3.54ASLNTTNLNIALTGLSVGMNVSNSEE744 pKa = 3.95PDD746 pKa = 3.44LQFALHH752 pKa = 6.6GLDD755 pKa = 4.27VDD757 pKa = 4.42LTSDD761 pKa = 4.72DD762 pKa = 3.4IVINGGLLSADD773 pKa = 3.44DD774 pKa = 4.13SGGTEE779 pKa = 3.98YY780 pKa = 11.11DD781 pKa = 3.04GTLTVKK787 pKa = 10.65VGEE790 pKa = 4.1FDD792 pKa = 3.22IAALASYY799 pKa = 10.85AMPPDD804 pKa = 4.18DD805 pKa = 4.64QSSLFAFAVIDD816 pKa = 4.18FPLGGPPAFFITGGAGGFGYY836 pKa = 10.6NRR838 pKa = 11.84DD839 pKa = 3.83LIMPNISSIMSFPLIEE855 pKa = 4.24IAQNPSKK862 pKa = 10.99YY863 pKa = 10.15KK864 pKa = 10.73NEE866 pKa = 4.02NLSSLLQTVQSAIPIEE882 pKa = 4.18QGQYY886 pKa = 8.65WLAAGINFNSYY897 pKa = 10.87GMVNSYY903 pKa = 10.9LLAAAAFGTQLEE915 pKa = 4.42LDD917 pKa = 4.08LLGVSTIVAPPLDD930 pKa = 4.79PDD932 pKa = 4.09PVAMIQFAIEE942 pKa = 4.16TTLVPAEE949 pKa = 4.16GLFSVEE955 pKa = 4.08GQLTNNSYY963 pKa = 10.98VLSKK967 pKa = 10.7NCHH970 pKa = 4.75LTGGFAFYY978 pKa = 10.6LWFGSNPHH986 pKa = 6.81AGDD989 pKa = 3.73FVISLGGYY997 pKa = 7.62SPKK1000 pKa = 10.16FKK1002 pKa = 10.62KK1003 pKa = 9.78PSYY1006 pKa = 9.59YY1007 pKa = 9.06PTVPLLGLNWQVCPEE1022 pKa = 4.36LSIQGGLYY1030 pKa = 10.37CAITSLAIMVGGSLKK1045 pKa = 10.77ALWQSDD1051 pKa = 4.19GIRR1054 pKa = 11.84AWFNLDD1060 pKa = 3.32ADD1062 pKa = 4.66VLLFWQPYY1070 pKa = 9.61QYY1072 pKa = 10.78TIDD1075 pKa = 3.75ANVDD1079 pKa = 3.17VGASLIIDD1087 pKa = 3.47VAFVNTAITAHH1098 pKa = 6.09TNADD1102 pKa = 3.62LSIWGPDD1109 pKa = 3.32FTGKK1113 pKa = 10.6AKK1115 pKa = 10.44VDD1117 pKa = 3.64LYY1119 pKa = 10.54IISFTISFGDD1129 pKa = 3.79SEE1131 pKa = 5.74PITTPPAAISWSNFKK1146 pKa = 10.71QSFLSTDD1153 pKa = 3.32PSNVCNLNLAHH1164 pKa = 6.79GVIKK1168 pKa = 10.7SDD1170 pKa = 4.34VSDD1173 pKa = 3.93QGITYY1178 pKa = 10.2DD1179 pKa = 3.61WIVDD1183 pKa = 3.55PEE1185 pKa = 4.28HH1186 pKa = 7.11FIFTTGSSIPSKK1198 pKa = 9.41TLSLNGVAQTLSTMDD1213 pKa = 3.33SSINTDD1219 pKa = 3.73FGVGMVRR1226 pKa = 11.84APSDD1230 pKa = 3.56SFTSDD1235 pKa = 2.69HH1236 pKa = 7.16AITLTYY1242 pKa = 10.64LDD1244 pKa = 4.15ADD1246 pKa = 3.71GNEE1249 pKa = 4.7PYY1251 pKa = 10.64DD1252 pKa = 3.61IADD1255 pKa = 3.9KK1256 pKa = 11.14FNISLLTSNVPQSLWQCITSLDD1278 pKa = 3.87DD1279 pKa = 4.52LEE1281 pKa = 4.62TSVASSSTTTIANTLSGISFSAKK1304 pKa = 8.26VTPADD1309 pKa = 3.28QVTPVALHH1317 pKa = 6.05NLLYY1321 pKa = 10.92SIDD1324 pKa = 3.97FEE1326 pKa = 4.53EE1327 pKa = 5.98DD1328 pKa = 2.92NLISYY1333 pKa = 7.75NKK1335 pKa = 9.57PSVPTTDD1342 pKa = 3.53GFTEE1346 pKa = 4.73EE1347 pKa = 4.21PCAEE1351 pKa = 4.06IGQTIAKK1358 pKa = 6.77NTSRR1362 pKa = 11.84TSILTRR1368 pKa = 11.84LQTTSFKK1375 pKa = 10.45IINDD1379 pKa = 3.32INVTSLSTEE1388 pKa = 3.58AANVYY1393 pKa = 9.98FVGDD1397 pKa = 4.71PILSYY1402 pKa = 10.86LGEE1405 pKa = 4.15EE1406 pKa = 4.3KK1407 pKa = 11.05VLGGASS1413 pKa = 3.13

Molecular weight: 151.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A077AYA7|A0A077AYA7_9PROT Holliday junction ATP-dependent DNA helicase RuvB OS=Candidatus Paracaedibacter acanthamoebae OX=91604 GN=ruvB PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.31VIAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1803

0

1803

547837

31

2490

303.8

34.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.805 ± 0.074

1.018 ± 0.019

5.146 ± 0.048

6.104 ± 0.065

4.251 ± 0.045

6.465 ± 0.071

2.369 ± 0.029

7.223 ± 0.046

6.354 ± 0.06

10.485 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.428 ± 0.029

4.355 ± 0.047

4.216 ± 0.043

4.378 ± 0.055

4.729 ± 0.047

6.753 ± 0.059

5.689 ± 0.044

5.89 ± 0.053

1.103 ± 0.022

3.237 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski