Pseudonocardia sp. HH130630-07

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Pseudonocardia; unclassified Pseudonocardia

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5619 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1ZMF5|A0A1B1ZMF5_9PSEU Uncharacterized protein OS=Pseudonocardia sp. HH130630-07 OX=1690815 GN=AFB00_12145 PE=3 SV=1
MM1 pKa = 7.22SAAAEE6 pKa = 4.29YY7 pKa = 9.97PVDD10 pKa = 4.0HH11 pKa = 6.62VLGDD15 pKa = 4.01DD16 pKa = 3.91VVDD19 pKa = 4.3VADD22 pKa = 5.08LVCPGCWSPVVDD34 pKa = 4.68VPPTGWPARR43 pKa = 11.84AGHH46 pKa = 7.12LPTFSHH52 pKa = 7.01EE53 pKa = 4.61DD54 pKa = 3.65GSVLCPDD61 pKa = 3.46EE62 pKa = 4.87AGRR65 pKa = 11.84VPDD68 pKa = 3.85PVEE71 pKa = 4.43AGGLRR76 pKa = 11.84YY77 pKa = 9.87ALTEE81 pKa = 4.04QGSGASLDD89 pKa = 3.77VDD91 pKa = 3.94RR92 pKa = 11.84EE93 pKa = 3.91WWAA96 pKa = 3.3

Molecular weight: 10.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1ZMQ0|A0A1B1ZMQ0_9PSEU Uncharacterized protein OS=Pseudonocardia sp. HH130630-07 OX=1690815 GN=AFB00_12630 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.3GRR40 pKa = 11.84NAVSAA45 pKa = 4.05

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5619

0

5619

1797081

28

9585

319.8

33.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.367 ± 0.064

0.723 ± 0.009

6.51 ± 0.035

5.186 ± 0.029

2.51 ± 0.02

10.159 ± 0.033

2.221 ± 0.017

2.916 ± 0.024

1.15 ± 0.021

10.324 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.583 ± 0.013

1.395 ± 0.013

6.529 ± 0.033

2.318 ± 0.02

8.739 ± 0.039

4.719 ± 0.026

6.114 ± 0.026

9.412 ± 0.035

1.44 ± 0.014

1.683 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski