Sewage-associated circular DNA molecule-2

Taxonomy: Viruses; Satellites; DNA satellites

Average proteome isoelectric point is 8.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7CL66|A0A0A7CL66_9VIRU Uncharacterized protein OS=Sewage-associated circular DNA molecule-2 OX=1595961 PE=4 SV=1
MM1 pKa = 6.88VQIIPNISQGTGDD14 pKa = 3.57NARR17 pKa = 11.84IGDD20 pKa = 3.98QIRR23 pKa = 11.84LQSLLIRR30 pKa = 11.84GYY32 pKa = 10.38IKK34 pKa = 10.92LDD36 pKa = 3.27INTSGASVSDD46 pKa = 3.97LSAVYY51 pKa = 9.82CRR53 pKa = 11.84LMVLSLKK60 pKa = 9.56TKK62 pKa = 10.43QNYY65 pKa = 8.26TDD67 pKa = 4.0ASSSATPLSNLLKK80 pKa = 10.75KK81 pKa = 10.9GGTTTSFSGVLSDD94 pKa = 4.81IYY96 pKa = 11.51APVNTDD102 pKa = 3.26LFTVHH107 pKa = 7.12AEE109 pKa = 3.51RR110 pKa = 11.84RR111 pKa = 11.84FYY113 pKa = 11.24LNQSMLQNFNSTTNAIVPIDD133 pKa = 3.49IKK135 pKa = 10.82NTVKK139 pKa = 10.54FFRR142 pKa = 11.84IPLKK146 pKa = 11.15VKK148 pKa = 10.54GKK150 pKa = 9.91LIKK153 pKa = 9.95YY154 pKa = 9.92DD155 pKa = 3.52SGISGGVLPTNYY167 pKa = 10.42GAFLVLGYY175 pKa = 10.67SFLNGASPDD184 pKa = 3.87TLSMRR189 pKa = 11.84VGMQFDD195 pKa = 4.39TIMNYY200 pKa = 10.24EE201 pKa = 4.07DD202 pKa = 3.26MM203 pKa = 5.56

Molecular weight: 22.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7CL66|A0A0A7CL66_9VIRU Uncharacterized protein OS=Sewage-associated circular DNA molecule-2 OX=1595961 PE=4 SV=1
MM1 pKa = 6.88VQIIPNISQGTGDD14 pKa = 3.57NARR17 pKa = 11.84IGDD20 pKa = 3.98QIRR23 pKa = 11.84LQSLLIRR30 pKa = 11.84GYY32 pKa = 10.38IKK34 pKa = 10.92LDD36 pKa = 3.27INTSGASVSDD46 pKa = 3.97LSAVYY51 pKa = 9.82CRR53 pKa = 11.84LMVLSLKK60 pKa = 9.56TKK62 pKa = 10.43QNYY65 pKa = 8.26TDD67 pKa = 4.0ASSSATPLSNLLKK80 pKa = 10.75KK81 pKa = 10.9GGTTTSFSGVLSDD94 pKa = 4.81IYY96 pKa = 11.51APVNTDD102 pKa = 3.26LFTVHH107 pKa = 7.12AEE109 pKa = 3.51RR110 pKa = 11.84RR111 pKa = 11.84FYY113 pKa = 11.24LNQSMLQNFNSTTNAIVPIDD133 pKa = 3.49IKK135 pKa = 10.82NTVKK139 pKa = 10.54FFRR142 pKa = 11.84IPLKK146 pKa = 11.15VKK148 pKa = 10.54GKK150 pKa = 9.91LIKK153 pKa = 9.95YY154 pKa = 9.92DD155 pKa = 3.52SGISGGVLPTNYY167 pKa = 10.42GAFLVLGYY175 pKa = 10.67SFLNGASPDD184 pKa = 3.87TLSMRR189 pKa = 11.84VGMQFDD195 pKa = 4.39TIMNYY200 pKa = 10.24EE201 pKa = 4.07DD202 pKa = 3.26MM203 pKa = 5.56

Molecular weight: 22.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

203

203

203

203.0

22.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.926 ± 0.0

0.493 ± 0.0

5.911 ± 0.0

0.985 ± 0.0

4.433 ± 0.0

7.882 ± 0.0

0.493 ± 0.0

7.882 ± 0.0

5.419 ± 0.0

10.837 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.448 ± 0.0

6.897 ± 0.0

3.448 ± 0.0

3.941 ± 0.0

3.941 ± 0.0

10.345 ± 0.0

7.882 ± 0.0

6.404 ± 0.0

0.0 ± 0.0

4.433 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski