Prevotella ruminicola (strain ATCC 19189 / JCM 8958 / 23)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; Prevotella ruminicola

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2761 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5EXR2|D5EXR2_PRER2 HipA domain protein OS=Prevotella ruminicola (strain ATCC 19189 / JCM 8958 / 23) OX=264731 GN=PRU_0523 PE=3 SV=1
MM1 pKa = 7.46KK2 pKa = 10.54NIIKK6 pKa = 10.52SSLLLLCSVAFLAACSDD23 pKa = 4.41DD24 pKa = 4.31NDD26 pKa = 5.49SNPTLQNPSTFTLNEE41 pKa = 3.86PGYY44 pKa = 11.1ASLIVDD50 pKa = 4.71LATSEE55 pKa = 5.0GIPFTWSQPDD65 pKa = 3.26YY66 pKa = 9.65GFPVAAEE73 pKa = 4.04YY74 pKa = 10.39QLQVSLDD81 pKa = 3.79GNFTTDD87 pKa = 3.37LADD90 pKa = 3.46VEE92 pKa = 4.81EE93 pKa = 5.02GNEE96 pKa = 3.96TTANFATLASYY107 pKa = 11.39YY108 pKa = 9.67NVPSGSIIPDD118 pKa = 3.36QLSNAINAMSGWDD131 pKa = 3.89GEE133 pKa = 4.79TVPQVATVYY142 pKa = 10.44VRR144 pKa = 11.84ATATTAGAPVIYY156 pKa = 10.52SNVVQIKK163 pKa = 8.61VVPNLVVAPSYY174 pKa = 11.31KK175 pKa = 10.3EE176 pKa = 3.73FIYY179 pKa = 10.42EE180 pKa = 3.94IGNEE184 pKa = 4.35SGWGTSYY191 pKa = 11.77AMRR194 pKa = 11.84SPDD197 pKa = 2.97QDD199 pKa = 3.67GIYY202 pKa = 10.76VSYY205 pKa = 10.97NWLDD209 pKa = 3.35GAFKK213 pKa = 10.48FKK215 pKa = 10.66PNEE218 pKa = 3.98NSWDD222 pKa = 3.87GDD224 pKa = 3.14WGQDD228 pKa = 3.4PNGDD232 pKa = 3.49FGTLVVDD239 pKa = 5.58GEE241 pKa = 4.37EE242 pKa = 4.53DD243 pKa = 3.63CNKK246 pKa = 10.34ADD248 pKa = 5.03GSFPDD253 pKa = 3.7NVQPAGFYY261 pKa = 10.02KK262 pKa = 10.4IQVSLVDD269 pKa = 4.06MTWSITPITNVSIIGGFNDD288 pKa = 3.35WAGDD292 pKa = 4.4VEE294 pKa = 4.72MTWNADD300 pKa = 3.31EE301 pKa = 5.05HH302 pKa = 6.79CWEE305 pKa = 4.41TTTSAVSGEE314 pKa = 4.05FKK316 pKa = 10.66FRR318 pKa = 11.84ANHH321 pKa = 5.77AWDD324 pKa = 4.06INWGGDD330 pKa = 3.32SFDD333 pKa = 4.21EE334 pKa = 4.82LKK336 pKa = 10.56QDD338 pKa = 4.04GANLSIDD345 pKa = 3.0AGTYY349 pKa = 10.04KK350 pKa = 10.53FQLYY354 pKa = 8.26LTYY357 pKa = 10.81DD358 pKa = 3.54GGSKK362 pKa = 10.41VVITAQQ368 pKa = 3.04

Molecular weight: 40.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5EYD7|D5EYD7_PRER2 30S ribosomal protein S18 OS=Prevotella ruminicola (strain ATCC 19189 / JCM 8958 / 23) OX=264731 GN=rpsR PE=3 SV=1
MM1 pKa = 7.02MALPAGSMAAANAGANPMGAPMGNPMANPMGNPMANPMGNPMGSPAGNPGVGAAVRR57 pKa = 11.84PGVNISVTPSASVSRR72 pKa = 11.84AANVPPAQQQQPVSPRR88 pKa = 11.84TSNAQNIAASISVGPAISIAQAGAGRR114 pKa = 11.84SSMPMMTLVNEE125 pKa = 4.33SLNIRR130 pKa = 11.84ITAMNGAIIGRR141 pKa = 11.84RR142 pKa = 11.84KK143 pKa = 10.03GPYY146 pKa = 9.0VQFFEE151 pKa = 4.41QNSYY155 pKa = 10.87VSGIHH160 pKa = 6.37AQLKK164 pKa = 8.05YY165 pKa = 10.7KK166 pKa = 10.63SGAGWFVIDD175 pKa = 4.2MNSSNGTRR183 pKa = 11.84INQAPIQPEE192 pKa = 4.18TEE194 pKa = 4.08NPLKK198 pKa = 11.09NGDD201 pKa = 3.82VLSIANVNLQVVIRR215 pKa = 4.31

Molecular weight: 22.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2761

0

2761

1065923

30

3655

386.1

43.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.596 ± 0.047

1.338 ± 0.017

5.956 ± 0.029

6.265 ± 0.041

4.275 ± 0.025

6.958 ± 0.037

2.08 ± 0.023

6.543 ± 0.04

6.455 ± 0.047

8.748 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.997 ± 0.02

4.979 ± 0.035

3.724 ± 0.023

3.966 ± 0.028

4.579 ± 0.035

5.602 ± 0.032

5.696 ± 0.037

6.491 ± 0.033

1.389 ± 0.02

4.364 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski