Enterobacteria phage P88

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Peduovirus; unclassified Peduovirus

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7NQ97|A0A0A7NQ97_9CAUD Gene D protein OS=Enterobacteria phage P88 OX=1567486 PE=4 SV=1
MM1 pKa = 7.42CFEE4 pKa = 5.3HH5 pKa = 8.58IEE7 pKa = 4.4DD8 pKa = 4.55FVADD12 pKa = 3.32IAVIFNWSPAEE23 pKa = 3.86IFMMTPGEE31 pKa = 4.27VVSWRR36 pKa = 11.84EE37 pKa = 3.52RR38 pKa = 11.84AALRR42 pKa = 11.84SGNADD47 pKa = 3.47NEE49 pKa = 4.46DD50 pKa = 3.64SS51 pKa = 3.73

Molecular weight: 5.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7NV51|A0A0A7NV51_9CAUD Carboxylate-amine ligase OS=Enterobacteria phage P88 OX=1567486 PE=4 SV=1
MM1 pKa = 7.87ANRR4 pKa = 11.84KK5 pKa = 5.77QHH7 pKa = 5.91RR8 pKa = 11.84AIAEE12 pKa = 3.84RR13 pKa = 11.84RR14 pKa = 11.84HH15 pKa = 5.49IQTEE19 pKa = 3.62INRR22 pKa = 11.84RR23 pKa = 11.84LFRR26 pKa = 11.84ASRR29 pKa = 11.84VAQIMHH35 pKa = 6.52INMLHH40 pKa = 5.78EE41 pKa = 4.85RR42 pKa = 11.84SHH44 pKa = 6.47ALSNIYY50 pKa = 9.96SAAVFSYY57 pKa = 10.68LADD60 pKa = 4.47DD61 pKa = 3.8LHH63 pKa = 6.93EE64 pKa = 4.45LQQLIQQQNKK74 pKa = 7.81LHH76 pKa = 6.28

Molecular weight: 9.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

10908

37

940

205.8

22.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.956 ± 0.431

0.917 ± 0.126

5.794 ± 0.266

6.399 ± 0.29

3.264 ± 0.18

6.949 ± 0.367

1.843 ± 0.187

5.482 ± 0.28

5.097 ± 0.289

8.48 ± 0.389

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.769 ± 0.155

4.18 ± 0.272

3.988 ± 0.178

4.345 ± 0.186

7.297 ± 0.352

6.051 ± 0.234

6.784 ± 0.356

6.17 ± 0.327

1.604 ± 0.156

2.631 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski