Actinophytocola oryzae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Actinophytocola

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9429 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R7VQS2|A0A4R7VQS2_9PSEU Threonine/homoserine/homoserine lactone efflux protein OS=Actinophytocola oryzae OX=502181 GN=CLV71_105111 PE=4 SV=1
MM1 pKa = 7.36SRR3 pKa = 11.84RR4 pKa = 11.84LIAALGAAVVVLGLVSMPASAGAAPPPQAATAAAAQTLTWTAGDD48 pKa = 3.62SQAAYY53 pKa = 9.21TSAPTTAVAGEE64 pKa = 4.11TTIVFEE70 pKa = 4.29NSEE73 pKa = 4.18ATGNTTGMSHH83 pKa = 7.0TLTFDD88 pKa = 3.08TSTPGYY94 pKa = 10.58NNDD97 pKa = 3.13VNVNILANPFDD108 pKa = 4.98ADD110 pKa = 3.72NGHH113 pKa = 6.71HH114 pKa = 6.17EE115 pKa = 4.62VTVTLTPGKK124 pKa = 10.12YY125 pKa = 8.99RR126 pKa = 11.84YY127 pKa = 9.22HH128 pKa = 6.95CVIPGHH134 pKa = 5.23STMTGEE140 pKa = 4.25FTVTGGGGGDD150 pKa = 3.97DD151 pKa = 3.56TTAPTVTATVAGDD164 pKa = 3.39QNGDD168 pKa = 2.99GDD170 pKa = 4.74YY171 pKa = 11.08VGSATVTLDD180 pKa = 3.11ATDD183 pKa = 3.58TGGSGVDD190 pKa = 3.28SVEE193 pKa = 3.95YY194 pKa = 10.58QLDD197 pKa = 3.57GGTWTTYY204 pKa = 6.41TAPVAVSAVGDD215 pKa = 3.64HH216 pKa = 5.34TVGYY220 pKa = 9.81RR221 pKa = 11.84ATDD224 pKa = 3.37VAGNTSTEE232 pKa = 3.92GATAFTVVAGGGGDD246 pKa = 3.47TTAPTVSGSVAGAQDD261 pKa = 3.46ADD263 pKa = 3.64GNYY266 pKa = 9.87VGSATVTATAADD278 pKa = 4.08EE279 pKa = 4.74GSGVASVEE287 pKa = 3.94YY288 pKa = 10.35QINDD292 pKa = 4.11LGWNPYY298 pKa = 6.53TAPVQVTNPGQYY310 pKa = 10.1VVLLRR315 pKa = 11.84ATDD318 pKa = 3.29AAGNVGSGQVSFEE331 pKa = 4.06VVAAPSDD338 pKa = 3.8TTPPTVNAEE347 pKa = 4.14VTGDD351 pKa = 3.57QNDD354 pKa = 3.77DD355 pKa = 3.11GDD357 pKa = 4.51YY358 pKa = 11.28VDD360 pKa = 4.13VATVTLSATDD370 pKa = 3.23TGSGVKK376 pKa = 10.01SVEE379 pKa = 3.89YY380 pKa = 10.81KK381 pKa = 10.67LDD383 pKa = 4.26DD384 pKa = 4.67GAWSAYY390 pKa = 7.8TAAIPVNAPGDD401 pKa = 3.63HH402 pKa = 5.22MVGYY406 pKa = 10.2RR407 pKa = 11.84ATDD410 pKa = 3.44NANNTSDD417 pKa = 3.13EE418 pKa = 4.23GMVMFAVVQEE428 pKa = 4.67DD429 pKa = 3.28ADD431 pKa = 3.97APTVTGQVVGQQDD444 pKa = 3.36ANGAYY449 pKa = 9.79VGSAMVTLTATDD461 pKa = 3.5AGSGVASVEE470 pKa = 4.06YY471 pKa = 10.69KK472 pKa = 10.68LDD474 pKa = 3.73GGAWTAYY481 pKa = 7.22TAAVQVSTPGAHH493 pKa = 4.36TVAYY497 pKa = 9.59RR498 pKa = 11.84ATDD501 pKa = 3.22EE502 pKa = 4.65AGNVSAEE509 pKa = 4.06GTLTFTVVAGGADD522 pKa = 3.38QTPPSVSALVSGTQNSSWEE541 pKa = 4.13FLDD544 pKa = 4.25GATITVTALDD554 pKa = 3.75TGSGVGSVEE563 pKa = 3.89YY564 pKa = 10.79KK565 pKa = 10.61LDD567 pKa = 3.74GGAWTAYY574 pKa = 6.1TAPVTVDD581 pKa = 2.83SAGDD585 pKa = 3.34HH586 pKa = 5.52TFSYY590 pKa = 10.61RR591 pKa = 11.84ATDD594 pKa = 3.37KK595 pKa = 11.39AGNTSAEE602 pKa = 4.13LSGSFTVVEE611 pKa = 4.74DD612 pKa = 4.48SPGQGPDD619 pKa = 3.13VCPSSDD625 pKa = 3.35VRR627 pKa = 11.84DD628 pKa = 3.6TVYY631 pKa = 11.12VGDD634 pKa = 4.32VDD636 pKa = 4.34SQVANVDD643 pKa = 3.52TGNGCTINDD652 pKa = 4.6VIDD655 pKa = 4.92EE656 pKa = 4.31DD657 pKa = 5.74AGWQTHH663 pKa = 5.67NDD665 pKa = 3.04FVRR668 pKa = 11.84YY669 pKa = 8.77VKK671 pKa = 10.61SVTKK675 pKa = 10.3EE676 pKa = 3.61LVQNGVIDD684 pKa = 3.51NDD686 pKa = 3.06ARR688 pKa = 11.84NRR690 pKa = 11.84IVAASIDD697 pKa = 3.91STVGGEE703 pKa = 4.16AAAKK707 pKa = 9.96AA708 pKa = 3.74

Molecular weight: 71.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R7VMJ9|A0A4R7VMJ9_9PSEU Sortase (Surface protein transpeptidase) OS=Actinophytocola oryzae OX=502181 GN=CLV71_106184 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILGARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.18GRR40 pKa = 11.84KK41 pKa = 8.86ALSAA45 pKa = 3.99

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9429

0

9429

3264173

29

9601

346.2

37.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.999 ± 0.043

0.779 ± 0.007

6.38 ± 0.024

5.325 ± 0.022

2.868 ± 0.014

9.179 ± 0.027

2.258 ± 0.011

3.125 ± 0.019

1.738 ± 0.02

10.355 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.76 ± 0.012

2.013 ± 0.02

5.899 ± 0.023

2.649 ± 0.018

7.983 ± 0.03

5.12 ± 0.018

6.514 ± 0.026

9.466 ± 0.032

1.562 ± 0.011

2.03 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski