Mycobacterium phage Zavala

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TRT0|A0A5J6TRT0_9CAUD Uncharacterized protein OS=Mycobacterium phage Zavala OX=2599887 GN=78 PE=4 SV=1
MM1 pKa = 7.28SRR3 pKa = 11.84HH4 pKa = 5.55YY5 pKa = 10.17CTGDD9 pKa = 3.68DD10 pKa = 3.52CWHH13 pKa = 6.5CEE15 pKa = 3.73RR16 pKa = 11.84RR17 pKa = 11.84ISQAEE22 pKa = 4.05YY23 pKa = 10.81EE24 pKa = 4.17RR25 pKa = 11.84DD26 pKa = 3.66CYY28 pKa = 11.44GDD30 pKa = 4.92DD31 pKa = 5.57DD32 pKa = 4.77YY33 pKa = 11.95PDD35 pKa = 4.53YY36 pKa = 11.71YY37 pKa = 11.27DD38 pKa = 3.5GTT40 pKa = 4.14

Molecular weight: 4.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TMV9|A0A5J6TMV9_9CAUD DnaE-like DNA polymerase III OS=Mycobacterium phage Zavala OX=2599887 GN=59 PE=4 SV=1
MM1 pKa = 7.35SSKK4 pKa = 10.33ILAHH8 pKa = 6.43KK9 pKa = 9.9RR10 pKa = 11.84QAARR14 pKa = 11.84DD15 pKa = 3.7QRR17 pKa = 11.84HH18 pKa = 5.44GEE20 pKa = 3.86RR21 pKa = 11.84LGAIVGVFLLHH32 pKa = 6.0ATMGAVGGLVGVAWVGLYY50 pKa = 10.47LGAPWW55 pKa = 4.05

Molecular weight: 5.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

18797

23

1277

193.8

21.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.986 ± 0.531

1.123 ± 0.14

6.607 ± 0.243

5.857 ± 0.251

2.586 ± 0.131

8.884 ± 0.443

2.144 ± 0.164

3.761 ± 0.143

3.389 ± 0.206

8.336 ± 0.213

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.192 ± 0.1

2.681 ± 0.162

5.708 ± 0.283

3.421 ± 0.151

7.182 ± 0.333

5.086 ± 0.194

5.767 ± 0.201

7.847 ± 0.234

2.16 ± 0.106

2.282 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski