Firmicutes bacterium CAG:882

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2572 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7BL48|R7BL48_9FIRM ATP synthase subunit c OS=Firmicutes bacterium CAG:882 OX=1262991 GN=atpE PE=3 SV=1
MM1 pKa = 7.79RR2 pKa = 11.84KK3 pKa = 9.08FKK5 pKa = 10.7KK6 pKa = 10.52VLASLIAGTLVLSNAVPVLAAQDD29 pKa = 3.93ASALPDD35 pKa = 3.12GTAYY39 pKa = 11.2LNINNSDD46 pKa = 3.4WQPFDD51 pKa = 4.66AEE53 pKa = 4.32WVNAEE58 pKa = 3.75ITGDD62 pKa = 3.4GSYY65 pKa = 9.44TVSMTAAEE73 pKa = 4.26AVDD76 pKa = 4.68LGAFNALEE84 pKa = 4.19VVNGEE89 pKa = 4.25EE90 pKa = 4.13VLGTGAVITVDD101 pKa = 3.76SIEE104 pKa = 4.38LNGEE108 pKa = 4.08EE109 pKa = 5.18IEE111 pKa = 4.44LQGSSYY117 pKa = 10.05TCSADD122 pKa = 3.2GLGVTTRR129 pKa = 11.84VNLYY133 pKa = 10.34NEE135 pKa = 4.4WNTPDD140 pKa = 3.27ATATAGDD147 pKa = 4.81DD148 pKa = 3.21KK149 pKa = 11.5HH150 pKa = 7.47ADD152 pKa = 3.43CRR154 pKa = 11.84VAEE157 pKa = 4.45GNVADD162 pKa = 4.59ATACLWTAEE171 pKa = 4.1QLTGVTSIVVNFTVSGFGTAGGPAEE196 pKa = 4.54AADD199 pKa = 3.81NSDD202 pKa = 3.29AATEE206 pKa = 4.19AVAHH210 pKa = 5.34LTINNEE216 pKa = 3.29NWGEE220 pKa = 4.04YY221 pKa = 7.62EE222 pKa = 4.27AEE224 pKa = 4.19YY225 pKa = 11.33VDD227 pKa = 4.28ATITGDD233 pKa = 3.23GTYY236 pKa = 9.15TVSMTAAEE244 pKa = 4.12AQNLGAYY251 pKa = 8.33NALQVPNGEE260 pKa = 4.74AIMGTACVLTIDD272 pKa = 4.13SIKK275 pKa = 10.81INGEE279 pKa = 3.98EE280 pKa = 4.01ITLQGPSYY288 pKa = 10.0TCSADD293 pKa = 3.18GAGVDD298 pKa = 3.69TRR300 pKa = 11.84VNIYY304 pKa = 10.7NEE306 pKa = 4.18YY307 pKa = 10.4NDD309 pKa = 4.27PDD311 pKa = 3.62ATATAGDD318 pKa = 4.8DD319 pKa = 3.3NHH321 pKa = 7.14LDD323 pKa = 3.62QRR325 pKa = 11.84IADD328 pKa = 4.13GNLADD333 pKa = 3.57ATARR337 pKa = 11.84LLSTDD342 pKa = 5.08DD343 pKa = 3.45IADD346 pKa = 3.73VQSVEE351 pKa = 4.11VTFTVSDD358 pKa = 3.75YY359 pKa = 11.75GKK361 pKa = 8.92MAEE364 pKa = 4.25AGEE367 pKa = 4.13AAGNDD372 pKa = 3.75GAFDD376 pKa = 4.45PNGEE380 pKa = 4.18YY381 pKa = 10.47NVYY384 pKa = 10.65FGVQTAAYY392 pKa = 9.4SFRR395 pKa = 11.84NAWNDD400 pKa = 3.23SYY402 pKa = 12.13GLGTPEE408 pKa = 4.23FDD410 pKa = 5.96QITGWNGNDD419 pKa = 2.85EE420 pKa = 4.51VVRR423 pKa = 11.84TGTITDD429 pKa = 3.69AVIKK433 pKa = 10.93GNGTYY438 pKa = 9.16TVSLTGVDD446 pKa = 4.27FADD449 pKa = 4.84DD450 pKa = 4.43AEE452 pKa = 4.39TLNLLFLSTDD462 pKa = 3.05IPLDD466 pKa = 3.63SDD468 pKa = 4.67AKK470 pKa = 10.03ITDD473 pKa = 3.33ITVKK477 pKa = 10.1MGGKK481 pKa = 9.17KK482 pKa = 10.44VYY484 pKa = 10.33DD485 pKa = 3.93FEE487 pKa = 5.68EE488 pKa = 5.27SYY490 pKa = 11.31QDD492 pKa = 4.52PDD494 pKa = 3.35TKK496 pKa = 11.11DD497 pKa = 3.35YY498 pKa = 11.34IKK500 pKa = 10.04TLLINTYY507 pKa = 10.07NDD509 pKa = 3.04AFNGEE514 pKa = 4.11NEE516 pKa = 4.05FAYY519 pKa = 9.63TLSAGMDD526 pKa = 3.2IEE528 pKa = 4.59VTFTVSGFAYY538 pKa = 9.94DD539 pKa = 4.17NPDD542 pKa = 3.45AVPSGDD548 pKa = 3.26TSATEE553 pKa = 3.97TTQAAEE559 pKa = 4.17TTKK562 pKa = 10.63AASEE566 pKa = 4.19ATTTASAEE574 pKa = 3.96ASQGGVSVGVIIAIVAAVVVVAAIVVVAVKK604 pKa = 10.27KK605 pKa = 10.74KK606 pKa = 10.17KK607 pKa = 10.34

Molecular weight: 63.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7BCH2|R7BCH2_9FIRM Extracellular solute-binding protein family 1 OS=Firmicutes bacterium CAG:882 OX=1262991 GN=BN803_01148 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 9.54QTFHH6 pKa = 6.38PKK8 pKa = 9.07KK9 pKa = 8.04RR10 pKa = 11.84QRR12 pKa = 11.84NKK14 pKa = 8.21VHH16 pKa = 6.58GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.98VLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.03GRR39 pKa = 11.84KK40 pKa = 8.97SLTVV44 pKa = 3.12

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2572

0

2572

845954

29

2973

328.9

36.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.614 ± 0.045

1.509 ± 0.019

6.284 ± 0.042

7.217 ± 0.048

4.07 ± 0.033

7.061 ± 0.044

1.625 ± 0.02

7.568 ± 0.045

6.674 ± 0.045

8.442 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.134 ± 0.024

4.883 ± 0.03

2.916 ± 0.026

2.607 ± 0.025

4.357 ± 0.038

6.006 ± 0.042

5.339 ± 0.046

7.228 ± 0.04

0.85 ± 0.017

4.613 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski