Plasmopara halstedii (Downy mildew of sunflower)

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae; Plasmopara

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15449 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N7L4B1|A0A0N7L4B1_PLAHL Pek protein kinase OS=Plasmopara halstedii OX=4781 PE=4 SV=1
MM1 pKa = 7.65IDD3 pKa = 4.31LRR5 pKa = 11.84FLLKK9 pKa = 10.64QFDD12 pKa = 4.71ANPSLDD18 pKa = 4.36DD19 pKa = 5.15DD20 pKa = 5.79SIDD23 pKa = 3.31SDD25 pKa = 3.81AYY27 pKa = 8.78YY28 pKa = 10.67SQVFFFLLVEE38 pKa = 4.4STFIPQMHH46 pKa = 7.3CIGG49 pKa = 3.82

Molecular weight: 5.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P1B5N0|A0A0P1B5N0_PLAHL Uncharacterized protein OS=Plasmopara halstedii OX=4781 PE=4 SV=1
MM1 pKa = 7.65RR2 pKa = 11.84RR3 pKa = 11.84SQTHH7 pKa = 5.78KK8 pKa = 10.56KK9 pKa = 9.98SIAGFSCSYY18 pKa = 11.04GGVTRR23 pKa = 11.84PTGGLRR29 pKa = 11.84SEE31 pKa = 4.16RR32 pKa = 11.84LTRR35 pKa = 11.84CWEE38 pKa = 3.96RR39 pKa = 11.84KK40 pKa = 8.05VVVDD44 pKa = 5.41AIVGGNVTYY53 pKa = 10.37EE54 pKa = 3.98GRR56 pKa = 11.84PMNIISDD63 pKa = 4.1VLWDD67 pKa = 3.9EE68 pKa = 4.39KK69 pKa = 11.26QPDD72 pKa = 3.82SGSNSHH78 pKa = 5.87QDD80 pKa = 2.84RR81 pKa = 11.84RR82 pKa = 11.84SIVCNHH88 pKa = 4.39QCTRR92 pKa = 11.84KK93 pKa = 10.39KK94 pKa = 10.63FMRR97 pKa = 11.84CLQEE101 pKa = 5.01ARR103 pKa = 11.84WQRR106 pKa = 11.84SRR108 pKa = 11.84GRR110 pKa = 3.41

Molecular weight: 12.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15449

0

15449

5873550

49

13799

380.2

42.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.648 ± 0.019

1.8 ± 0.012

5.611 ± 0.016

6.405 ± 0.022

3.964 ± 0.016

5.337 ± 0.028

2.542 ± 0.009

5.104 ± 0.016

5.634 ± 0.022

9.809 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.543 ± 0.009

4.183 ± 0.011

4.128 ± 0.014

4.335 ± 0.019

6.013 ± 0.018

8.464 ± 0.026

5.846 ± 0.018

6.774 ± 0.016

1.135 ± 0.007

2.711 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski