Nostoc sp. Peltigera malacea cyanobiont DB3992

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Nostocaceae; Nostoc; unclassified Nostoc

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6320 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D0HL85|A0A2D0HL85_9NOSO Uncharacterized protein OS=Nostoc sp. 'Peltigera malacea cyanobiont' DB3992 OX=1206980 GN=CK516_09725 PE=4 SV=1
MM1 pKa = 7.47IVQDD5 pKa = 4.12EE6 pKa = 4.59FDD8 pKa = 3.93NQASAQEE15 pKa = 4.09EE16 pKa = 4.22FEE18 pKa = 4.64EE19 pKa = 4.74YY20 pKa = 10.53VDD22 pKa = 3.98HH23 pKa = 7.16LADD26 pKa = 3.5AVAPEE31 pKa = 4.21IEE33 pKa = 4.53IDD35 pKa = 4.59SVPDD39 pKa = 3.85DD40 pKa = 4.07LGEE43 pKa = 5.07LYY45 pKa = 10.51RR46 pKa = 11.84VWNGSQLLGTFYY58 pKa = 11.27QNLEE62 pKa = 4.46GKK64 pKa = 9.19WIAQPCNSDD73 pKa = 4.22KK74 pKa = 10.96RR75 pKa = 11.84HH76 pKa = 6.2CCDD79 pKa = 3.38TSDD82 pKa = 4.31SALLLIIAVNGLLIADD98 pKa = 4.06VAA100 pKa = 4.17

Molecular weight: 11.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D0HBY3|A0A2D0HBY3_9NOSO Biotin carboxylase OS=Nostoc sp. 'Peltigera malacea cyanobiont' DB3992 OX=1206980 GN=CK516_29565 PE=4 SV=1
MM1 pKa = 7.43SIKK4 pKa = 10.57RR5 pKa = 11.84MLASAALILPIAVTFIPFQADD26 pKa = 3.11AQIRR30 pKa = 11.84RR31 pKa = 11.84PVRR34 pKa = 11.84RR35 pKa = 11.84SVPTRR40 pKa = 11.84IIRR43 pKa = 11.84RR44 pKa = 11.84PVRR47 pKa = 11.84VRR49 pKa = 11.84VHH51 pKa = 5.99TPVQARR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 11.84IVVPGRR65 pKa = 11.84WVQTNRR71 pKa = 11.84GRR73 pKa = 11.84RR74 pKa = 11.84WIPARR79 pKa = 11.84SVYY82 pKa = 10.25RR83 pKa = 3.73

Molecular weight: 9.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6320

0

6320

1697874

25

2914

268.7

29.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.966 ± 0.036

0.975 ± 0.01

4.867 ± 0.021

6.303 ± 0.03

4.02 ± 0.019

6.601 ± 0.037

1.811 ± 0.015

6.885 ± 0.027

4.944 ± 0.03

11.016 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.793 ± 0.012

4.546 ± 0.026

4.591 ± 0.025

5.329 ± 0.03

5.027 ± 0.026

6.515 ± 0.025

5.701 ± 0.022

6.59 ± 0.027

1.405 ± 0.014

3.114 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski