Arthrobacter phage Sputnik

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2KJA0|A0A3G2KJA0_9CAUD Portal protein OS=Arthrobacter phage Sputnik OX=2419969 GN=4 PE=4 SV=1
MM1 pKa = 7.5AGNWADD7 pKa = 3.38AARR10 pKa = 11.84YY11 pKa = 8.53IAEE14 pKa = 4.2SLEE17 pKa = 4.24TTGYY21 pKa = 8.19NAKK24 pKa = 10.37VNAQDD29 pKa = 4.48LNIPGFWVTPATRR42 pKa = 11.84TFDD45 pKa = 3.81RR46 pKa = 11.84LDD48 pKa = 3.41ASTATLTFDD57 pKa = 3.59VYY59 pKa = 11.48AVVSPMPNAEE69 pKa = 4.0AALEE73 pKa = 4.17EE74 pKa = 4.42LSDD77 pKa = 3.83MQDD80 pKa = 3.02ALIGLGEE87 pKa = 4.29TQPEE91 pKa = 4.19LRR93 pKa = 11.84GQGLDD98 pKa = 3.46AEE100 pKa = 4.85VTQVQLSNKK109 pKa = 8.25TPEE112 pKa = 4.44PLPALKK118 pKa = 9.0ITITVEE124 pKa = 4.05DD125 pKa = 4.02EE126 pKa = 3.96

Molecular weight: 13.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2KJ75|A0A3G2KJ75_9CAUD Minor tail protein OS=Arthrobacter phage Sputnik OX=2419969 GN=14 PE=4 SV=1
MM1 pKa = 6.48QQTRR5 pKa = 11.84FTNNDD10 pKa = 2.84WPQLSVEE17 pKa = 4.29PDD19 pKa = 3.34GLGQWLVLSGGVGLGSFPDD38 pKa = 3.39KK39 pKa = 10.66RR40 pKa = 11.84SAFNFARR47 pKa = 11.84GRR49 pKa = 11.84AVARR53 pKa = 11.84ASCSGGVPARR63 pKa = 11.84VLVVGAA69 pKa = 4.55

Molecular weight: 7.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23

0

23

4508

29

616

196.0

21.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.957 ± 0.93

0.555 ± 0.23

5.235 ± 0.381

5.28 ± 0.566

3.283 ± 0.15

9.339 ± 0.695

1.531 ± 0.257

5.457 ± 0.567

5.169 ± 0.326

7.897 ± 0.368

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.862 ± 0.246

3.594 ± 0.28

4.414 ± 0.318

3.926 ± 0.273

5.213 ± 0.643

6.211 ± 0.282

7.054 ± 0.397

7.254 ± 0.426

1.686 ± 0.179

2.085 ± 0.304
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski