Litomosoides sigmodontis (Filarial nematode worm)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Spirurina; Spiruromorpha; Filarioidea; Onchocercidae; Litomosoides

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9894 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P6T6M8|A0A3P6T6M8_LITSI PIH1 domain-containing protein OS=Litomosoides sigmodontis OX=42156 GN=NLS_LOCUS5043 PE=3 SV=1
MM1 pKa = 7.61RR2 pKa = 11.84LIKK5 pKa = 9.48TRR7 pKa = 11.84HH8 pKa = 4.99FLRR11 pKa = 11.84VFSFYY16 pKa = 10.54LTGIHH21 pKa = 7.19SDD23 pKa = 4.06DD24 pKa = 4.91DD25 pKa = 5.29DD26 pKa = 5.79NDD28 pKa = 4.26GSDD31 pKa = 3.38VCYY34 pKa = 10.92DD35 pKa = 3.98NNNNDD40 pKa = 3.95CDD42 pKa = 4.31NDD44 pKa = 3.95NGDD47 pKa = 5.15DD48 pKa = 5.5DD49 pKa = 6.5DD50 pKa = 6.54DD51 pKa = 5.99NSDD54 pKa = 3.82NDD56 pKa = 4.0GGTDD60 pKa = 3.56DD61 pKa = 6.24DD62 pKa = 4.81EE63 pKa = 4.76WNCRR67 pKa = 11.84CNNVII72 pKa = 4.65

Molecular weight: 8.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P6SXX4|A0A3P6SXX4_LITSI Uncharacterized protein OS=Litomosoides sigmodontis OX=42156 GN=NLS_LOCUS4787 PE=4 SV=1
MM1 pKa = 8.06RR2 pKa = 11.84MMFVTPAFLSVYY14 pKa = 10.17LIAFSSLTITALFALDD30 pKa = 3.88EE31 pKa = 4.39PQWWKK36 pKa = 10.99VYY38 pKa = 9.17WIHH41 pKa = 7.57SSLLEE46 pKa = 4.31VKK48 pKa = 10.57GLLDD52 pKa = 3.56KK53 pKa = 10.69PLYY56 pKa = 10.38RR57 pKa = 11.84ILQKK61 pKa = 10.98DD62 pKa = 3.28PALINVLKK70 pKa = 10.53EE71 pKa = 3.85ALQDD75 pKa = 3.69TLVEE79 pKa = 4.29CSRR82 pKa = 11.84QSVIHH87 pKa = 6.05HH88 pKa = 6.52WKK90 pKa = 10.32CNVDD94 pKa = 3.43GAPRR98 pKa = 11.84KK99 pKa = 10.02ALFSNVASFASKK111 pKa = 10.14EE112 pKa = 3.87FAYY115 pKa = 10.67FLALSSAAAVRR126 pKa = 11.84AIAHH130 pKa = 5.63ACARR134 pKa = 11.84GRR136 pKa = 11.84LRR138 pKa = 11.84SCSCDD143 pKa = 3.23PSKK146 pKa = 10.62IGPVHH151 pKa = 7.07ADD153 pKa = 3.98QRR155 pKa = 11.84DD156 pKa = 3.17TWARR160 pKa = 11.84CSVDD164 pKa = 3.21RR165 pKa = 11.84GSDD168 pKa = 3.17NLQYY172 pKa = 11.0AVKK175 pKa = 10.23LSKK178 pKa = 10.67RR179 pKa = 11.84LIDD182 pKa = 3.48RR183 pKa = 11.84QFACSYY189 pKa = 9.4SDD191 pKa = 3.49RR192 pKa = 11.84VAQIYY197 pKa = 8.61LHH199 pKa = 6.55NIAVGRR205 pKa = 11.84SRR207 pKa = 11.84VALHH211 pKa = 6.2IKK213 pKa = 9.42CYY215 pKa = 9.86CVSAFGSCRR224 pKa = 11.84RR225 pKa = 11.84RR226 pKa = 11.84HH227 pKa = 5.98CEE229 pKa = 3.52AKK231 pKa = 10.17VVPFEE236 pKa = 4.16MTGDD240 pKa = 3.55AVMKK244 pKa = 10.41SLLRR248 pKa = 11.84SRR250 pKa = 11.84RR251 pKa = 11.84IRR253 pKa = 11.84RR254 pKa = 11.84SNSLPTTRR262 pKa = 11.84VTCLRR267 pKa = 11.84PRR269 pKa = 11.84NEE271 pKa = 3.77RR272 pKa = 11.84NMRR275 pKa = 11.84RR276 pKa = 11.84RR277 pKa = 11.84VVPLWFIDD285 pKa = 3.51TRR287 pKa = 11.84SWTMRR292 pKa = 11.84SGKK295 pKa = 7.96SRR297 pKa = 11.84RR298 pKa = 11.84RR299 pKa = 11.84LKK301 pKa = 10.82NN302 pKa = 3.05

Molecular weight: 34.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9894

0

9894

4320305

10

7030

436.7

49.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.528 ± 0.02

2.236 ± 0.018

5.466 ± 0.016

6.671 ± 0.026

4.096 ± 0.016

5.258 ± 0.025

2.414 ± 0.009

6.082 ± 0.016

6.021 ± 0.021

9.26 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.464 ± 0.012

4.863 ± 0.014

4.293 ± 0.023

4.094 ± 0.019

5.934 ± 0.02

8.357 ± 0.027

5.508 ± 0.02

6.196 ± 0.016

1.1 ± 0.008

3.099 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski