Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; Rhodospirillum; Rhodospirillum rubrum

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3835 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q2RYG6|Q2RYG6_RHORT Peptidase OS=Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1) OX=269796 GN=Rru_A0024 PE=4 SV=1
MM1 pKa = 7.74PYY3 pKa = 10.48VVTEE7 pKa = 3.81NCIKK11 pKa = 10.51CKK13 pKa = 10.63YY14 pKa = 7.8QDD16 pKa = 3.86CVEE19 pKa = 4.24VCPVDD24 pKa = 3.79CFYY27 pKa = 11.23EE28 pKa = 4.55GEE30 pKa = 4.2NFLVINPDD38 pKa = 2.99EE39 pKa = 5.52CIDD42 pKa = 4.08CGVCEE47 pKa = 4.44PEE49 pKa = 4.91CPAEE53 pKa = 4.84AIFPDD58 pKa = 3.83SEE60 pKa = 5.31AIAGKK65 pKa = 9.0WLEE68 pKa = 4.12INRR71 pKa = 11.84KK72 pKa = 8.47FADD75 pKa = 3.78LWPNITRR82 pKa = 11.84KK83 pKa = 10.45GPALADD89 pKa = 3.69ADD91 pKa = 3.96DD92 pKa = 4.53WKK94 pKa = 11.19DD95 pKa = 3.65KK96 pKa = 10.37PDD98 pKa = 3.43KK99 pKa = 10.83TGLLSEE105 pKa = 4.51NPGKK109 pKa = 9.72GTVV112 pKa = 3.13

Molecular weight:
12.46 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q2RP35|Q2RP35_RHORT Phage integrase OS=Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1) OX=269796 GN=Rru_A3316 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.73GFRR19 pKa = 11.84SRR21 pKa = 11.84LATVGGRR28 pKa = 11.84RR29 pKa = 11.84VLANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.85GRR39 pKa = 11.84KK40 pKa = 8.87RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight:
5.16 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3835

0

3835

1292602

20

1981

337.1

36.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.276 ± 0.061

0.913 ± 0.013

5.797 ± 0.031

5.424 ± 0.035

3.403 ± 0.023

9.077 ± 0.041

1.969 ± 0.019

4.716 ± 0.027

2.789 ± 0.031

11.205 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.262 ± 0.019

2.048 ± 0.02

5.684 ± 0.036

2.823 ± 0.023

7.552 ± 0.041

5.084 ± 0.029

5.288 ± 0.031

7.499 ± 0.035

1.259 ± 0.015

1.934 ± 0.019

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski