Bat coronavirus HKU9-2

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Nobecovirus; Rousettus bat coronavirus HKU9

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3EXH7|A3EXH7_BCHK9 Membrane protein OS=Bat coronavirus HKU9-2 OX=424368 GN=M PE=3 SV=1
MM1 pKa = 7.39YY2 pKa = 9.81EE3 pKa = 4.19FVSADD8 pKa = 3.09TSVIVTNVLVIIILCLFVVIVGCALLLILQFILGTCSCLFSIICKK53 pKa = 8.67PTILVYY59 pKa = 11.09NKK61 pKa = 9.81FKK63 pKa = 11.29NEE65 pKa = 3.87SLLNEE70 pKa = 4.09QEE72 pKa = 4.4EE73 pKa = 4.49LLSASVV79 pKa = 3.16

Molecular weight: 8.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A3EXH9|A3EXH9_BCHK9 Uncharacterized protein NS7a OS=Bat coronavirus HKU9-2 OX=424368 GN=NS7a PE=4 SV=1
MM1 pKa = 7.62SGRR4 pKa = 11.84NKK6 pKa = 9.53PRR8 pKa = 11.84PGNQSPKK15 pKa = 9.1VTFKK19 pKa = 11.01QEE21 pKa = 3.89SDD23 pKa = 3.52GSDD26 pKa = 3.37SEE28 pKa = 4.52SEE30 pKa = 3.92RR31 pKa = 11.84RR32 pKa = 11.84NGNRR36 pKa = 11.84NGARR40 pKa = 11.84PKK42 pKa = 10.56NNNNRR47 pKa = 11.84GFGSKK52 pKa = 9.96PEE54 pKa = 4.14KK55 pKa = 10.15PKK57 pKa = 10.61AAPPQNVSWYY67 pKa = 10.16APLVQTGKK75 pKa = 10.87ADD77 pKa = 3.41LRR79 pKa = 11.84FPRR82 pKa = 11.84GEE84 pKa = 4.24GVPISQGVDD93 pKa = 3.36PIYY96 pKa = 10.73QHH98 pKa = 6.86GYY100 pKa = 3.79WTRR103 pKa = 11.84TQRR106 pKa = 11.84SFQKK110 pKa = 10.64GGKK113 pKa = 6.62TVQANPRR120 pKa = 11.84WYY122 pKa = 10.03FYY124 pKa = 9.78YY125 pKa = 10.35TGTGRR130 pKa = 11.84YY131 pKa = 9.43ADD133 pKa = 4.32LRR135 pKa = 11.84WGQKK139 pKa = 10.07NPDD142 pKa = 3.59IVWVGEE148 pKa = 3.68EE149 pKa = 3.95GANINRR155 pKa = 11.84VGDD158 pKa = 3.5MGTRR162 pKa = 11.84NPNNDD167 pKa = 2.84AAIAVQLGNGIPKK180 pKa = 10.22GFYY183 pKa = 10.29AEE185 pKa = 4.4GRR187 pKa = 11.84GSRR190 pKa = 11.84GNSRR194 pKa = 11.84NSSRR198 pKa = 11.84NSSRR202 pKa = 11.84ASSRR206 pKa = 11.84SSSRR210 pKa = 11.84PNSRR214 pKa = 11.84GASPGRR220 pKa = 11.84NTPNPGAGSEE230 pKa = 3.79PWMAYY235 pKa = 9.72LVSKK239 pKa = 10.94LEE241 pKa = 3.94NLEE244 pKa = 4.11AKK246 pKa = 10.8VNGTKK251 pKa = 10.31PEE253 pKa = 4.0TKK255 pKa = 10.52APVQVTKK262 pKa = 10.97NAAAEE267 pKa = 3.96NAKK270 pKa = 10.26KK271 pKa = 10.1LRR273 pKa = 11.84HH274 pKa = 5.99KK275 pKa = 8.81RR276 pKa = 11.84TPHH279 pKa = 6.14KK280 pKa = 10.85GSGVTMNFGRR290 pKa = 11.84RR291 pKa = 11.84GPGDD295 pKa = 3.41LEE297 pKa = 5.04GNFGDD302 pKa = 3.87QTMLKK307 pKa = 10.26LGTDD311 pKa = 3.79DD312 pKa = 4.12PRR314 pKa = 11.84FPAAAQMAPNTAAFLFMSHH333 pKa = 6.93FSTRR337 pKa = 11.84EE338 pKa = 3.53EE339 pKa = 5.28GDD341 pKa = 4.05ALWLNYY347 pKa = 9.66KK348 pKa = 10.28GAIKK352 pKa = 10.38LPKK355 pKa = 9.83DD356 pKa = 3.6DD357 pKa = 4.76PNYY360 pKa = 8.83NQWVKK365 pKa = 10.63ILEE368 pKa = 4.41EE369 pKa = 3.88NLNAYY374 pKa = 9.62KK375 pKa = 10.44SFPPPEE381 pKa = 4.09PKK383 pKa = 9.43KK384 pKa = 10.51DD385 pKa = 3.38KK386 pKa = 10.52KK387 pKa = 10.83KK388 pKa = 10.78KK389 pKa = 10.06EE390 pKa = 4.12EE391 pKa = 4.12TSSEE395 pKa = 4.27TVVFEE400 pKa = 4.57DD401 pKa = 4.59ASTGTDD407 pKa = 3.32QPVVKK412 pKa = 10.41VWVKK416 pKa = 11.22DD417 pKa = 3.4EE418 pKa = 4.28GMQTDD423 pKa = 4.35DD424 pKa = 3.55EE425 pKa = 4.58WLGGDD430 pKa = 3.25DD431 pKa = 3.26TVYY434 pKa = 10.88EE435 pKa = 4.19EE436 pKa = 5.68EE437 pKa = 3.91EE438 pKa = 4.54DD439 pKa = 3.59KK440 pKa = 11.38PKK442 pKa = 10.3VQRR445 pKa = 11.84RR446 pKa = 11.84HH447 pKa = 5.49KK448 pKa = 10.52KK449 pKa = 9.6RR450 pKa = 11.84GSTASRR456 pKa = 11.84VTVADD461 pKa = 3.66PTNLAAEE468 pKa = 4.6RR469 pKa = 11.84SS470 pKa = 3.91

Molecular weight: 51.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

9522

79

6903

1190.3

132.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.383 ± 0.446

3.193 ± 0.322

5.219 ± 0.656

3.77 ± 0.389

4.558 ± 0.423

6.385 ± 0.377

1.838 ± 0.241

4.127 ± 0.305

4.925 ± 0.696

9.599 ± 0.729

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.342 ± 0.282

5.072 ± 0.777

4.579 ± 0.436

3.319 ± 0.32

4.054 ± 0.547

6.868 ± 0.531

6.574 ± 0.42

9.882 ± 0.554

1.334 ± 0.214

4.978 ± 0.364
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski