Paenibacillus phage Likha

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fernvirus; Paenibacillus virus Likha

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I7SDI3|A0A2I7SDI3_9CAUD Transcriptional regulator OS=Paenibacillus phage Likha OX=2070193 GN=LIKHA_57 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.05IKK4 pKa = 10.36YY5 pKa = 8.81RR6 pKa = 11.84LSIGYY11 pKa = 8.57PGAVRR16 pKa = 11.84EE17 pKa = 4.52DD18 pKa = 3.58EE19 pKa = 4.69VEE21 pKa = 3.93IDD23 pKa = 3.95DD24 pKa = 5.19EE25 pKa = 4.32EE26 pKa = 6.18LEE28 pKa = 4.26GLTPEE33 pKa = 4.09EE34 pKa = 4.6AEE36 pKa = 5.63DD37 pKa = 4.53RR38 pKa = 11.84ICDD41 pKa = 3.35IVNEE45 pKa = 4.18HH46 pKa = 5.74VQDD49 pKa = 5.35FISLSWEE56 pKa = 4.02EE57 pKa = 3.99VEE59 pKa = 4.75EE60 pKa = 4.21

Molecular weight: 7.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I7SDD3|A0A2I7SDD3_9CAUD Terminase small subunit OS=Paenibacillus phage Likha OX=2070193 GN=LIKHA_1 PE=4 SV=1
MM1 pKa = 7.82ANIQVLGVPEE11 pKa = 4.26TVRR14 pKa = 11.84KK15 pKa = 9.82IGLFEE20 pKa = 4.15MEE22 pKa = 4.45RR23 pKa = 11.84KK24 pKa = 8.73QAAIVLVKK32 pKa = 9.65KK33 pKa = 8.6TATSIQKK40 pKa = 9.43EE41 pKa = 4.65GKK43 pKa = 9.42SLAPSSPAGRR53 pKa = 11.84KK54 pKa = 8.81KK55 pKa = 10.99SKK57 pKa = 10.48GKK59 pKa = 9.74PGDD62 pKa = 3.55LKK64 pKa = 10.89RR65 pKa = 11.84SIRR68 pKa = 11.84PKK70 pKa = 10.24YY71 pKa = 9.56MEE73 pKa = 5.22GGLSATVVPRR83 pKa = 11.84KK84 pKa = 9.51PKK86 pKa = 9.03GAHH89 pKa = 5.02RR90 pKa = 11.84HH91 pKa = 4.01LVEE94 pKa = 3.84YY95 pKa = 7.99GTRR98 pKa = 11.84QRR100 pKa = 11.84RR101 pKa = 11.84NKK103 pKa = 10.06KK104 pKa = 8.57GANRR108 pKa = 11.84GKK110 pKa = 9.41MPKK113 pKa = 9.78KK114 pKa = 9.07PFMSIAEE121 pKa = 3.97RR122 pKa = 11.84HH123 pKa = 5.43AEE125 pKa = 3.58GRR127 pKa = 11.84YY128 pKa = 9.23NKK130 pKa = 9.55EE131 pKa = 3.54LEE133 pKa = 4.5RR134 pKa = 11.84IFSRR138 pKa = 11.84DD139 pKa = 2.98EE140 pKa = 4.15TII142 pKa = 4.43

Molecular weight: 15.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

12557

44

878

193.2

22.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.387 ± 0.423

0.828 ± 0.108

5.806 ± 0.211

8.434 ± 0.469

3.695 ± 0.194

6.244 ± 0.322

1.529 ± 0.142

7.343 ± 0.318

9.302 ± 0.347

7.972 ± 0.245

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.787 ± 0.195

4.412 ± 0.168

3.281 ± 0.222

4.205 ± 0.188

4.778 ± 0.377

6.363 ± 0.288

5.423 ± 0.23

6.084 ± 0.201

1.521 ± 0.175

3.608 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski