Acutalibacter sp. 1XD8-33

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Acutalibacter; unclassified Acutalibacter

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3386 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A9EFT4|A0A3A9EFT4_9FIRM Phosphate-specific transport system accessory protein PhoU OS=Acutalibacter sp. 1XD8-33 OX=2320081 GN=phoU PE=3 SV=1
MM1 pKa = 7.73SGGDD5 pKa = 3.71SRR7 pKa = 11.84NLCLVFQEE15 pKa = 4.53YY16 pKa = 11.25GDD18 pKa = 3.84ILFSEE23 pKa = 5.03MIPGEE28 pKa = 4.11ALSKK32 pKa = 11.01CEE34 pKa = 4.62SALKK38 pKa = 10.85YY39 pKa = 9.22NTEE42 pKa = 3.59DD43 pKa = 2.82WYY45 pKa = 10.59IQVDD49 pKa = 5.23LIDD52 pKa = 5.21SIAACMMMNGQTDD65 pKa = 3.4EE66 pKa = 4.3SLQEE70 pKa = 4.11FAWLLWTLAEE80 pKa = 5.37DD81 pKa = 4.88DD82 pKa = 4.6IKK84 pKa = 11.29DD85 pKa = 3.85AEE87 pKa = 4.52IKK89 pKa = 10.38FQLGNNLVCVLDD101 pKa = 3.9SDD103 pKa = 4.23PEE105 pKa = 3.95EE106 pKa = 4.15EE107 pKa = 4.15IEE109 pKa = 4.24YY110 pKa = 10.72KK111 pKa = 10.63EE112 pKa = 4.52SLFDD116 pKa = 4.36LVKK119 pKa = 11.07DD120 pKa = 3.82DD121 pKa = 3.94VTVLSYY127 pKa = 10.72AIHH130 pKa = 6.2YY131 pKa = 7.65FRR133 pKa = 6.41

Molecular weight: 15.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A9EH58|A0A3A9EH58_9FIRM XRE family transcriptional regulator OS=Acutalibacter sp. 1XD8-33 OX=2320081 GN=D7X94_15325 PE=4 SV=1
MM1 pKa = 7.3KK2 pKa = 10.27RR3 pKa = 11.84GFFHH7 pKa = 7.5CMRR10 pKa = 11.84LLGILAVAMVLAAAGPCRR28 pKa = 11.84EE29 pKa = 4.26VEE31 pKa = 3.83AGAPVSRR38 pKa = 11.84GGAPDD43 pKa = 3.4FHH45 pKa = 7.75QVGVACWSLVGAGAMGVAAAALAGGRR71 pKa = 11.84PKK73 pKa = 10.45SGKK76 pKa = 9.96LLVPASVEE84 pKa = 3.87GQNPVKK90 pKa = 10.31LDD92 pKa = 3.1HH93 pKa = 6.05STRR96 pKa = 11.84YY97 pKa = 9.64VPPPHH102 pKa = 6.55RR103 pKa = 11.84WNYY106 pKa = 10.07RR107 pKa = 11.84SSQRR111 pKa = 11.84RR112 pKa = 11.84WW113 pKa = 2.91

Molecular weight: 11.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3386

0

3386

976833

33

2614

288.5

32.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.945 ± 0.048

1.632 ± 0.019

5.37 ± 0.032

7.362 ± 0.046

4.014 ± 0.028

7.931 ± 0.042

1.759 ± 0.023

5.515 ± 0.035

5.24 ± 0.037

9.707 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.728 ± 0.018

3.505 ± 0.029

4.372 ± 0.035

3.757 ± 0.027

5.87 ± 0.05

5.765 ± 0.036

5.091 ± 0.041

6.708 ± 0.039

1.152 ± 0.016

3.575 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski