Hepatitis B virus genotype D subtype ayw (isolate France/Tiollais/1979) (HBV-D)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Blubervirales; Hepadnaviridae; Orthohepadnavirus; Hepatitis B virus; HBV genotype D

Average proteome isoelectric point is 8.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>sp|P0C573|HBEAG_HBVD3 External core antigen OS=Hepatitis B virus genotype D subtype ayw (isolate France/Tiollais/1979) OX=490133 GN=C PE=1 SV=1
MM1 pKa = 7.82AARR4 pKa = 11.84LCCQLDD10 pKa = 3.48PARR13 pKa = 11.84DD14 pKa = 3.92VLCLRR19 pKa = 11.84PVGAEE24 pKa = 3.54SRR26 pKa = 11.84GRR28 pKa = 11.84PFSGSLGTLSSPSPSAVPTDD48 pKa = 3.82HH49 pKa = 7.0GAHH52 pKa = 6.51LSLRR56 pKa = 11.84GLPVCAFSSAGPCALRR72 pKa = 11.84FTSARR77 pKa = 11.84RR78 pKa = 11.84MEE80 pKa = 4.46TTVNAHH86 pKa = 6.06QILPKK91 pKa = 10.13VLHH94 pKa = 6.33KK95 pKa = 10.15RR96 pKa = 11.84TLGLSAMSTTDD107 pKa = 3.32LEE109 pKa = 5.55AYY111 pKa = 10.39FKK113 pKa = 11.19DD114 pKa = 4.14CLFKK118 pKa = 10.77DD119 pKa = 3.51WEE121 pKa = 4.27EE122 pKa = 3.98LGEE125 pKa = 4.19EE126 pKa = 3.71IRR128 pKa = 11.84LKK130 pKa = 11.04VFVLGGCRR138 pKa = 11.84HH139 pKa = 6.33KK140 pKa = 10.74LVCAPAPCNFFTSAA154 pKa = 3.82

Molecular weight:
16.62 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>sp|P03165|X_HBVD3 Protein X OS=Hepatitis B virus genotype D subtype ayw (isolate France/Tiollais/1979) OX=490133 GN=X PE=1 SV=2
MM1 pKa = 7.99DD2 pKa = 4.14RR3 pKa = 11.84QRR5 pKa = 11.84SRR7 pKa = 11.84KK8 pKa = 9.49GSTHH12 pKa = 6.91ALMAHH17 pKa = 6.46DD18 pKa = 4.38QAPASGGCVSKK29 pKa = 10.79HH30 pKa = 6.02LAQTWPLPGNGVKK43 pKa = 10.39VQVLFTQKK51 pKa = 11.14GLVSWRR57 pKa = 11.84EE58 pKa = 4.02SEE60 pKa = 4.3SLLRR64 pKa = 11.84LNTCIQRR71 pKa = 11.84HH72 pKa = 4.37QRR74 pKa = 11.84RR75 pKa = 11.84ITTLCKK81 pKa = 10.17RR82 pKa = 11.84GSKK85 pKa = 6.99TQKK88 pKa = 7.48THH90 pKa = 6.65NSLTYY95 pKa = 9.7FPINRR100 pKa = 11.84PVNRR104 pKa = 11.84KK105 pKa = 8.3FSKK108 pKa = 9.63TFFDD112 pKa = 3.85FLYY115 pKa = 10.83DD116 pKa = 3.45VFLWQGPITSNDD128 pKa = 2.96ITHH131 pKa = 7.45KK132 pKa = 10.31II133 pKa = 3.51

Molecular weight:
15.3 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

2

9

2505

95

832

278.3

31.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.429 ± 0.713

3.473 ± 0.587

2.874 ± 0.25

2.715 ± 0.623

5.389 ± 0.389

6.547 ± 0.407

2.794 ± 0.792

4.032 ± 0.403

2.635 ± 0.633

12.136 ± 0.655

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.956 ± 0.32

3.234 ± 0.542

8.583 ± 0.533

3.952 ± 0.338

6.667 ± 1.027

10.14 ± 0.264

6.667 ± 0.806

5.11 ± 0.346

3.034 ± 0.596

2.635 ± 0.375

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski