Changjiang tombus-like virus 10

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFU0|A0A1L3KFU0_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 10 OX=1922803 PE=4 SV=1
MM1 pKa = 7.55LSGVQGPIKK10 pKa = 10.22FLIHH14 pKa = 6.36NNSATNLRR22 pKa = 11.84RR23 pKa = 11.84ALMEE27 pKa = 3.66RR28 pKa = 11.84VYY30 pKa = 10.62HH31 pKa = 6.07VEE33 pKa = 4.87ANGQLQRR40 pKa = 11.84PPQPMPGIYY49 pKa = 8.84ATLTALRR56 pKa = 11.84DD57 pKa = 4.06KK58 pKa = 10.46IAHH61 pKa = 6.06RR62 pKa = 11.84VGAATPMEE70 pKa = 3.76RR71 pKa = 11.84HH72 pKa = 5.65YY73 pKa = 10.9FVSTRR78 pKa = 11.84PADD81 pKa = 3.24KK82 pKa = 10.69RR83 pKa = 11.84RR84 pKa = 11.84IYY86 pKa = 10.39EE87 pKa = 3.61NAYY90 pKa = 10.53ASLQLTPPSVKK101 pKa = 10.13DD102 pKa = 3.27ARR104 pKa = 11.84VYY106 pKa = 10.71GAFVKK111 pKa = 10.65CEE113 pKa = 4.26KK114 pKa = 10.46INASKK119 pKa = 10.8KK120 pKa = 9.74SDD122 pKa = 3.53PAPRR126 pKa = 11.84IIQPRR131 pKa = 11.84SPRR134 pKa = 11.84YY135 pKa = 8.12NIEE138 pKa = 3.57VGRR141 pKa = 11.84YY142 pKa = 8.35LAVIEE147 pKa = 4.41HH148 pKa = 7.05DD149 pKa = 3.77VYY151 pKa = 11.58AAIDD155 pKa = 4.09EE156 pKa = 4.74LWGSTTVMKK165 pKa = 10.49GYY167 pKa = 10.12NVEE170 pKa = 4.16QIGEE174 pKa = 4.23IISAKK179 pKa = 9.21WGEE182 pKa = 4.03FTKK185 pKa = 10.63PVALGLDD192 pKa = 3.57ASRR195 pKa = 11.84FDD197 pKa = 3.23QHH199 pKa = 8.34VSVEE203 pKa = 4.09ALEE206 pKa = 4.58FEE208 pKa = 4.49HH209 pKa = 7.02GLYY212 pKa = 8.78NTIFKK217 pKa = 10.66SRR219 pKa = 11.84EE220 pKa = 3.64LRR222 pKa = 11.84RR223 pKa = 11.84LLRR226 pKa = 11.84WQLDD230 pKa = 3.66NRR232 pKa = 11.84GVAHH236 pKa = 7.39ADD238 pKa = 3.2DD239 pKa = 3.75ATFVYY244 pKa = 9.89RR245 pKa = 11.84KK246 pKa = 8.9PGSRR250 pKa = 11.84MSGDD254 pKa = 3.32MNTALGNIIIMCILVKK270 pKa = 9.79QYY272 pKa = 10.98CDD274 pKa = 3.12EE275 pKa = 4.41KK276 pKa = 11.08GIRR279 pKa = 11.84AEE281 pKa = 4.85LINNGDD287 pKa = 3.72DD288 pKa = 3.43CTLIFEE294 pKa = 4.88AEE296 pKa = 4.42HH297 pKa = 6.18VDD299 pKa = 3.27AMRR302 pKa = 11.84DD303 pKa = 3.63GLHH306 pKa = 6.07EE307 pKa = 3.92WFLRR311 pKa = 11.84YY312 pKa = 9.67GFNIVEE318 pKa = 4.11EE319 pKa = 4.8PIVDD323 pKa = 4.09VIEE326 pKa = 4.88KK327 pKa = 10.31IEE329 pKa = 4.24FCQMHH334 pKa = 6.73PVLVGDD340 pKa = 3.8SYY342 pKa = 12.27KK343 pKa = 9.95MVRR346 pKa = 11.84NFWASLSKK354 pKa = 10.67DD355 pKa = 3.32AISIRR360 pKa = 11.84SRR362 pKa = 11.84SITEE366 pKa = 3.71LQQWMHH372 pKa = 6.31CVGKK376 pKa = 10.45CGLAAASGVPIQQAYY391 pKa = 9.64YY392 pKa = 10.62SYY394 pKa = 10.88FVRR397 pKa = 11.84NGVKK401 pKa = 10.18GKK403 pKa = 10.44RR404 pKa = 11.84LDD406 pKa = 3.71QVEE409 pKa = 4.67GRR411 pKa = 11.84CGLTWFSRR419 pKa = 11.84GLHH422 pKa = 5.47ATTTHH427 pKa = 5.8ITDD430 pKa = 3.55STRR433 pKa = 11.84HH434 pKa = 4.87SFYY437 pKa = 10.88LAFGIHH443 pKa = 6.99ADD445 pKa = 3.47MQFALEE451 pKa = 4.12AEE453 pKa = 4.35YY454 pKa = 11.21DD455 pKa = 3.87RR456 pKa = 11.84LVLSTNAPKK465 pKa = 10.78AEE467 pKa = 4.33DD468 pKa = 3.91YY469 pKa = 10.7IVYY472 pKa = 10.1YY473 pKa = 10.9

Molecular weight: 53.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFS5|A0A1L3KFS5_9VIRU RNA-directed RNA polymerase OS=Changjiang tombus-like virus 10 OX=1922803 PE=4 SV=1
MM1 pKa = 7.63ANKK4 pKa = 8.31LTKK7 pKa = 9.44ATRR10 pKa = 11.84KK11 pKa = 9.28VKK13 pKa = 10.33RR14 pKa = 11.84AAKK17 pKa = 9.66RR18 pKa = 11.84VAKK21 pKa = 9.92AAKK24 pKa = 9.58PYY26 pKa = 10.41LPKK29 pKa = 10.74FNVKK33 pKa = 10.07KK34 pKa = 10.73GLACLDD40 pKa = 3.66PAGQKK45 pKa = 8.93AANMLLDD52 pKa = 3.79PCNAQLSPAAYY63 pKa = 10.08RR64 pKa = 11.84GDD66 pKa = 3.25QGYY69 pKa = 7.63KK70 pKa = 9.14TRR72 pKa = 11.84FVSNGSIGQAPNSTCAGIIFTPSLGRR98 pKa = 11.84VYY100 pKa = 10.79SADD103 pKa = 3.28ATTSTATVTWNVGSFPNPGQNFMQGNADD131 pKa = 4.01SIRR134 pKa = 11.84SLGACLSCYY143 pKa = 9.48PVSSVMNTSGFVYY156 pKa = 9.88TGIVPEE162 pKa = 4.36SAALAATTLDD172 pKa = 4.78GLSQLCNRR180 pKa = 11.84TSRR183 pKa = 11.84IQVAEE188 pKa = 3.88PMEE191 pKa = 4.52TKK193 pKa = 10.39FVPGAGDD200 pKa = 3.32EE201 pKa = 4.55VYY203 pKa = 10.99NPVGGSIQNDD213 pKa = 3.74NDD215 pKa = 3.65TMCIVFVFTGFSSASGIRR233 pKa = 11.84IRR235 pKa = 11.84ATNIIEE241 pKa = 4.23WKK243 pKa = 9.15PDD245 pKa = 3.45PSLGIVSEE253 pKa = 4.33SHH255 pKa = 5.66LTNPSVNTIEE265 pKa = 4.15HH266 pKa = 5.66VKK268 pKa = 10.46RR269 pKa = 11.84CLFEE273 pKa = 4.95QDD275 pKa = 3.57PHH277 pKa = 6.09WFSSVGNIVKK287 pKa = 8.97RR288 pKa = 11.84TVGGYY293 pKa = 8.3MSGGIAGAVFGGLGSLSLL311 pKa = 3.98

Molecular weight: 32.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1001

217

473

333.7

37.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.191 ± 0.318

1.798 ± 0.358

4.995 ± 0.645

5.594 ± 1.357

3.796 ± 0.205

7.692 ± 0.94

2.198 ± 0.722

5.894 ± 0.669

4.895 ± 0.148

7.992 ± 1.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.298 ± 0.334

4.396 ± 0.707

4.296 ± 0.666

3.397 ± 0.12

7.393 ± 1.672

6.693 ± 1.222

5.195 ± 0.768

7.592 ± 0.439

1.299 ± 0.14

3.397 ± 0.93
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski