marine gamma proteobacterium HTCC2143

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Spongiibacteraceae; unclassified Spongiibacteraceae; BD1-7 clade

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3661 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0YFZ0|A0YFZ0_9GAMM Uncharacterized protein OS=marine gamma proteobacterium HTCC2143 OX=247633 GN=GP2143_01815 PE=4 SV=1
GG1 pKa = 7.44GDD3 pKa = 3.11VDD5 pKa = 4.2YY6 pKa = 10.8TSLDD10 pKa = 3.08IVRR13 pKa = 11.84TEE15 pKa = 4.14DD16 pKa = 3.2SLEE19 pKa = 4.26IEE21 pKa = 4.51TVTQEE26 pKa = 4.19FRR28 pKa = 11.84LTSTAGEE35 pKa = 4.25SFDD38 pKa = 3.37WMVGGFIFQEE48 pKa = 4.7DD49 pKa = 4.17IEE51 pKa = 4.77TDD53 pKa = 3.11GSTFFGNDD61 pKa = 2.3FRR63 pKa = 11.84AYY65 pKa = 10.57GDD67 pKa = 3.53VLFGAFPNILSTVEE81 pKa = 4.21LLSGLPAGAILSSDD95 pKa = 3.4NVASTAFEE103 pKa = 4.01QDD105 pKa = 2.66NFAYY109 pKa = 10.63SLFATGDD116 pKa = 3.29YY117 pKa = 10.95HH118 pKa = 6.8FTDD121 pKa = 4.19KK122 pKa = 10.4LTATLGVSYY131 pKa = 9.63TYY133 pKa = 10.51DD134 pKa = 3.19EE135 pKa = 4.87KK136 pKa = 11.44EE137 pKa = 4.3VVKK140 pKa = 10.79DD141 pKa = 3.22SYY143 pKa = 11.6RR144 pKa = 11.84NNDD147 pKa = 3.34LYY149 pKa = 11.5SAVDD153 pKa = 3.77LVPVLGATPLAGLIPSLQGLQFRR176 pKa = 11.84PPVIDD181 pKa = 3.51YY182 pKa = 8.85PNSVEE187 pKa = 4.61NGEE190 pKa = 4.19SDD192 pKa = 3.75DD193 pKa = 6.55DD194 pKa = 4.1KK195 pKa = 11.2VTWTARR201 pKa = 11.84VTYY204 pKa = 9.65QVNDD208 pKa = 3.6MVNVYY213 pKa = 10.57ASAATGYY220 pKa = 10.49KK221 pKa = 9.83SSSWNIGGASRR232 pKa = 11.84PFPASEE238 pKa = 4.07AALDD242 pKa = 3.86AAGLLVANQSFGTRR256 pKa = 11.84FASPEE261 pKa = 3.62EE262 pKa = 3.83AMVYY266 pKa = 10.36EE267 pKa = 5.02LGLKK271 pKa = 10.42ASFEE275 pKa = 4.03QGAFNIAIFDD285 pKa = 3.57QTIEE289 pKa = 4.75DD290 pKa = 4.06FQQNAFDD297 pKa = 4.46GDD299 pKa = 4.36SFVLTNAGEE308 pKa = 3.95RR309 pKa = 11.84STRR312 pKa = 11.84GIEE315 pKa = 3.82FDD317 pKa = 3.77ALYY320 pKa = 10.78SPTDD324 pKa = 2.75AWLFNLAGTFMDD336 pKa = 4.75PKK338 pKa = 9.77YY339 pKa = 10.89DD340 pKa = 3.59SYY342 pKa = 11.55TGASGIGGAPIDD354 pKa = 4.27LSGEE358 pKa = 4.08EE359 pKa = 3.69PAGIHH364 pKa = 6.12KK365 pKa = 10.72EE366 pKa = 4.01NVTASATYY374 pKa = 10.36RR375 pKa = 11.84FTVGDD380 pKa = 4.75GITGYY385 pKa = 10.67VRR387 pKa = 11.84TDD389 pKa = 3.28YY390 pKa = 10.61IYY392 pKa = 10.84EE393 pKa = 4.18SDD395 pKa = 5.48VRR397 pKa = 11.84AAWNVPEE404 pKa = 4.16EE405 pKa = 4.2FSRR408 pKa = 11.84EE409 pKa = 3.85VSTWNASAGLQFTNGVNVQVWGRR432 pKa = 11.84NINDD436 pKa = 3.42DD437 pKa = 3.76EE438 pKa = 5.51YY439 pKa = 11.49YY440 pKa = 10.6VGVFQGVVQSGTYY453 pKa = 9.78NAFASAPATYY463 pKa = 9.68GVNVSYY469 pKa = 10.9EE470 pKa = 3.98FF471 pKa = 4.22

Molecular weight: 51.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0YDP3|A0YDP3_9GAMM Uncharacterized protein OS=marine gamma proteobacterium HTCC2143 OX=247633 GN=GP2143_10032 PE=4 SV=1
MM1 pKa = 7.39RR2 pKa = 11.84QGIGDD7 pKa = 3.7NPEE10 pKa = 4.08GQEE13 pKa = 4.05SAKK16 pKa = 10.27SGRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84MRR23 pKa = 11.84APHH26 pKa = 4.91YY27 pKa = 9.85QGYY30 pKa = 8.22PSQYY34 pKa = 8.31NTSITCRR41 pKa = 11.84VSGLPPKK48 pKa = 10.61ARR50 pKa = 11.84LKK52 pKa = 10.93KK53 pKa = 9.76LTFDD57 pKa = 4.07LGQSVRR63 pKa = 11.84HH64 pKa = 4.99VQNNVTTRR72 pKa = 11.84CTTVSFGIKK81 pKa = 10.19GPIVAAGVRR90 pKa = 11.84VMDD93 pKa = 4.27FVMLLFGYY101 pKa = 9.34FLSRR105 pKa = 11.84RR106 pKa = 11.84FYY108 pKa = 10.71FF109 pKa = 4.02

Molecular weight: 12.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3661

0

3661

1126783

20

2218

307.8

33.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.192 ± 0.04

1.04 ± 0.015

5.946 ± 0.03

5.968 ± 0.032

3.959 ± 0.027

7.451 ± 0.04

2.146 ± 0.021

6.431 ± 0.035

4.472 ± 0.03

10.064 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.648 ± 0.021

3.945 ± 0.026

4.184 ± 0.026

4.139 ± 0.033

5.162 ± 0.029

6.756 ± 0.031

5.307 ± 0.028

7.026 ± 0.034

1.285 ± 0.016

2.881 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski