Proteome-pI 2.0 - Proteome Isoelectric Point Database

Pseudocercospora fijiensis (strain CIRAD86) (Black leaf streak disease fungus) (Mycosphaerella fijiensis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Pseudocercospora; Pseudocercospora fijiensis

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 13062 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|M3B4A1|M3B4A1_PSEFD PPM-type phosphatase domain-containing protein OS=Pseudocercospora fijiensis (strain CIRAD86) OX=383855 GN=MYCFIDRAFT_133445 PE=3 SV=1

NN1 pKa = 7.92DD2 pKa = 4.59CGDD5 pKa = 3.51SSFVKK10 pKa = 9.31VTMSNRR16 pKa = 11.84PLVADD21 pKa = 4.38CQQLVANLAGNQEE34 pKa = 3.97WRR36 pKa = 11.84VTTSGVQVAVYY47 pKa = 7.25QTCAFNAVSFGGDD60 pKa = 3.66SIIGNADD67 pKa = 3.63VIDD70 pKa = 5.67LINDD74 pKa = 4.27SISKK78 pKa = 10.73FEE80 pKa = 4.69DD81 pKa = 2.85NGYY84 pKa = 10.76VGAQGG89 pKa = 3.31

Molecular weight: 9.38 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|M3AH58|M3AH58_PSEFD Uncharacterized protein OS=Pseudocercospora fijiensis (strain CIRAD86) OX=383855 GN=MYCFIDRAFT_214540 PE=4 SV=1

MM1 pKa = 7.44VLLLLLLIQRR11 pKa = 11.84LLQRR15 pKa = 11.84LLQRR19 pKa = 11.84LLQLQIPVVQVLVVLVLLLLLLIQRR44 pKa = 11.84LLQLQILVVQALVVQVLLLLLLIQRR69 pKa = 11.84LLQLQILVAQALMAQALVVQALVVQALVVQVLVVQALVVQALVVQALVVQALVVQVLVVQALVVQALVVRR139 pKa = 11.84APVVRR144 pKa = 11.84APVVRR149 pKa = 11.84APVVQALVVQALVVQALVVQALVVQALVVQALVVQAPVVRR189 pKa = 11.84ALVVQALVVQALQIPKK205 pKa = 9.68HH206 pKa = 5.51LHH208 pKa = 4.78QQSQLPNRR216 pKa = 11.84KK217 pKa = 8.7IPKK220 pKa = 8.6HH221 pKa = 4.39LHH223 pKa = 5.5RR224 pKa = 11.84LRR226 pKa = 11.84RR227 pKa = 11.84WAPPLMIMLRR237 pKa = 11.84FTAA240 pKa = 4.71

Molecular weight: 26.45 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
13043	19	13062	5586011	49	6685	427.7	47.46

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
8.921 ± 0.017	1.436 ± 0.01	5.559 ± 0.016	6.106 ± 0.021	3.665 ± 0.014	6.722 ± 0.02	2.63 ± 0.011	4.83 ± 0.014	4.959 ± 0.021	8.839 ± 0.024

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.236 ± 0.008	3.618 ± 0.013	5.835 ± 0.02	4.17 ± 0.015	6.401 ± 0.022	8.04 ± 0.023	5.89 ± 0.017	5.877 ± 0.015	1.523 ± 0.008	2.744 ± 0.012

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski