Streptomyces roseus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6493 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J6XKE8|A0A0J6XKE8_9ACTN RpiR family transcriptional regulator OS=Streptomyces roseus OX=66430 GN=ACS04_25170 PE=4 SV=1
MM1 pKa = 7.86TDD3 pKa = 2.98TGQVPGEE10 pKa = 4.23GLPDD14 pKa = 3.36SAGMVDD20 pKa = 3.98QQGMPAPVQHH30 pKa = 6.97PAPAPVQGGYY40 pKa = 10.96AFQDD44 pKa = 3.68LVEE47 pKa = 4.89NPAEE51 pKa = 4.4PEE53 pKa = 4.01DD54 pKa = 4.12EE55 pKa = 4.64EE56 pKa = 4.84LLLMPSGQGAWSDD69 pKa = 3.78PQVVPPAPVFPVDD82 pKa = 3.62TSYY85 pKa = 12.13GDD87 pKa = 3.33APYY90 pKa = 11.05AEE92 pKa = 4.79YY93 pKa = 10.26PDD95 pKa = 4.29AQAAYY100 pKa = 9.47AEE102 pKa = 4.37PGYY105 pKa = 9.77PVFPDD110 pKa = 4.03PSYY113 pKa = 11.81ADD115 pKa = 3.53PSYY118 pKa = 11.37SAGAHH123 pKa = 4.95EE124 pKa = 5.01AGGRR128 pKa = 11.84DD129 pKa = 3.94SGALDD134 pKa = 3.57LGGLVVPPPAAAPAAAAAPVTPPAPARR161 pKa = 11.84RR162 pKa = 11.84PLHH165 pKa = 5.96MGPPVPEE172 pKa = 4.06TTGGVVRR179 pKa = 11.84SLADD183 pKa = 3.86RR184 pKa = 11.84GPAAAPAAPVVPVAQVAAPAAAPVAAPSVPIPVQVAGPPTLGPEE228 pKa = 4.23YY229 pKa = 10.69LDD231 pKa = 3.48VPRR234 pKa = 11.84PP235 pKa = 3.47

Molecular weight: 23.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J7AM98|A0A0J7AM98_9ACTN Transcriptional regulatory protein AfsQ1 OS=Streptomyces roseus OX=66430 GN=ACS04_08065 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.34GRR40 pKa = 11.84AALSAA45 pKa = 3.96

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6493

0

6493

2083404

27

5456

320.9

34.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.282 ± 0.051

0.795 ± 0.008

5.662 ± 0.023

5.717 ± 0.031

2.738 ± 0.016

9.675 ± 0.032

2.239 ± 0.015

3.149 ± 0.023

2.23 ± 0.025

10.36 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.763 ± 0.013

1.748 ± 0.019

6.228 ± 0.027

2.752 ± 0.017

7.877 ± 0.035

4.939 ± 0.022

5.911 ± 0.027

8.317 ± 0.027

1.519 ± 0.012

2.099 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski