Aggregatibacter phage S1249

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D0UIN2|D0UIN2_9CAUD Uncharacterized protein OS=Aggregatibacter phage S1249 OX=683735 GN=D11S_2270 PE=4 SV=1
MM1 pKa = 7.8DD2 pKa = 6.51LIEE5 pKa = 4.99GMSRR9 pKa = 11.84DD10 pKa = 3.64DD11 pKa = 4.73SMTSLHH17 pKa = 6.75CSGDD21 pKa = 3.33ANSYY25 pKa = 9.87YY26 pKa = 10.42RR27 pKa = 11.84VTEE30 pKa = 4.0TPEE33 pKa = 4.36EE34 pKa = 3.92ILEE37 pKa = 4.28TMKK40 pKa = 10.49PAEE43 pKa = 4.05

Molecular weight: 4.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D0UIM2|D0UIM2_9CAUD Uncharacterized protein OS=Aggregatibacter phage S1249 OX=683735 GN=D11S_2260 PE=4 SV=1
MM1 pKa = 7.28NGWGKK6 pKa = 8.44LTLRR10 pKa = 11.84CEE12 pKa = 4.52CLRR15 pKa = 11.84KK16 pKa = 9.81CKK18 pKa = 9.99QSQWVGIAKK27 pKa = 10.0RR28 pKa = 11.84KK29 pKa = 8.49QPNHH33 pKa = 5.69TFLSVFKK40 pKa = 10.58LVKK43 pKa = 10.1NGKK46 pKa = 8.52ATDD49 pKa = 3.53SKK51 pKa = 11.29RR52 pKa = 3.32

Molecular weight: 6.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

13153

38

757

199.3

22.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.447 ± 0.343

1.042 ± 0.141

5.991 ± 0.217

6.341 ± 0.286

4.151 ± 0.186

6.47 ± 0.275

1.589 ± 0.142

6.957 ± 0.245

7.603 ± 0.324

8.698 ± 0.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.129 ± 0.14

5.428 ± 0.281

2.927 ± 0.177

5.094 ± 0.189

4.6 ± 0.25

6.219 ± 0.257

5.824 ± 0.213

6.014 ± 0.205

1.475 ± 0.132

3.003 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski