Curtobacterium sp. MR_MD2014

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Curtobacterium; unclassified Curtobacterium

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2675 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N7A0C9|A0A0N7A0C9_9MICO Phosphoglucosamine mutase OS=Curtobacterium sp. MR_MD2014 OX=1561023 GN=glmM PE=3 SV=1
MM1 pKa = 7.3GNAARR6 pKa = 11.84GAVVVAGAVATVVALSGCSVLGAGTRR32 pKa = 11.84TAEE35 pKa = 4.16APAPSASATTAATPGTASCDD55 pKa = 3.62LASGTGSVSIEE66 pKa = 4.08EE67 pKa = 3.99TADD70 pKa = 3.11AYY72 pKa = 10.34RR73 pKa = 11.84VEE75 pKa = 4.35WSGVPTDD82 pKa = 4.84DD83 pKa = 4.9DD84 pKa = 4.05GARR87 pKa = 11.84QEE89 pKa = 4.24YY90 pKa = 9.45QVQLGTASQDD100 pKa = 3.35DD101 pKa = 5.12HH102 pKa = 6.35ITMYY106 pKa = 10.54IEE108 pKa = 4.24FDD110 pKa = 4.16SITGATTYY118 pKa = 10.78GWFDD122 pKa = 4.7PDD124 pKa = 3.88TLDD127 pKa = 3.68VDD129 pKa = 4.59PVPGDD134 pKa = 3.83PDD136 pKa = 3.53TSAGTVVGVFPKK148 pKa = 10.46QRR150 pKa = 11.84DD151 pKa = 2.82IDD153 pKa = 3.57ASYY156 pKa = 10.33WSPSWSKK163 pKa = 7.82TTTEE167 pKa = 4.93DD168 pKa = 4.6SDD170 pKa = 3.86TTYY173 pKa = 9.87CTQDD177 pKa = 3.26GQVLDD182 pKa = 4.17WTPLDD187 pKa = 3.53

Molecular weight: 19.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N7A3V3|A0A0N7A3V3_9MICO Pseudouridine synthase OS=Curtobacterium sp. MR_MD2014 OX=1561023 GN=NI26_05465 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 10.04KK16 pKa = 8.86HH17 pKa = 4.36GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.68GRR40 pKa = 11.84TEE42 pKa = 4.13LSAA45 pKa = 4.86

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2675

0

2675

840519

29

2642

314.2

33.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.513 ± 0.068

0.455 ± 0.01

6.657 ± 0.048

5.281 ± 0.053

2.969 ± 0.036

9.204 ± 0.05

2.039 ± 0.024

3.672 ± 0.043

1.892 ± 0.04

9.587 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.634 ± 0.019

1.755 ± 0.024

5.351 ± 0.036

2.947 ± 0.032

7.448 ± 0.058

5.436 ± 0.033

6.644 ± 0.06

10.187 ± 0.059

1.479 ± 0.024

1.849 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski